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Eef1akmt4-ece2 (NM_177941) Mouse Untagged Clone

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SKU
MC222543
Ece2 (untagged) - Mouse endothelin converting enzyme 2 (Ece2), transcript variant 1, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $917.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Eef1akmt4-ece2
Synonyms 1810009K13Rik; 6330509A19Rik; 9630025D12Rik; BB127715; Ece-2; Eef1akmt4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222543 representing NM_177941
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCTCCCCGGACTCCGGTGTCCCCTCCGGAGTTACCGGAGAAGAACTTCCAGTACCGCCAAGTCC
AGTACTGGGACCAGCGCTACAAAGACGCGGCCGACTCTGGACCCTACGAGTGGTTCGGAGACTTCGCTTC
CTTCCGTGCCCTCCTAGAGCCGGAGCTGTGTCCTGAGGACCGTATCCTCGTGCTAGGTTGCGGGAACAGT
GCCTTGAGCTATGAGCTGTTCCTTGGAGGCTTCCCTAATGTGACCAGTGTGGATTACTCTCCAGTAGTGG
TGGCAGCCATGCAAGTTCGCTATGCCCATGTACCCAGTCTACGCTGGGAGACCATGGATGTTCGAGCACT
GGACTTCCCTAGTGGGTCTTTTGATGTTGTCCTCGAGAAGGGTACTCTGGATGCCATGCTGGCTGGGGAG
CCAGATCCCTGGAATGTATCTTCTGAAGGTGTCCACACTGTGGACCAGGTGCTGAGTGAGATGGTGGAGT
ACAAACGTGCTAAGCTTCGGGATGAAGAATCACCAGAGATTACAGTTGAAGGCAGGGCCACCCGGGACTC
ACTGGAGGTGGGATTCCAGAAGAGGACAAGACAACTGTTTGGTTCACACACACAGTTGGAGCTGGTCTTG
GCAGGCCTCATTCTAGTGTTGGCTGCCCTTCTTTTGGGCTGCCTCGTGGCTCTGTGGGTCCACAGAGACC
CAGCCCATAGCACCTGCGTCACAGAAGCCTGCATTCGAGTAGCTGGAAAAATCCTGGAGTCTCTAGACCG
TGGGGTGAGCCCCTGTCAGGACTTTTACCAGTTCTCCTGTGGAGGCTGGATTCGAAGAAACCCTCTACCC
AATGGACGTTCTCGCTGGAACACCTTCAACAGCCTCTGGGACCAGAACCAGGCCATACTGAAGCACCTAC
TTGAGAACACCACTTTCAATTCCAGCAGTGAAGCTGAGAGGAAGACTCGGAGTTTCTACCTGTCCTGCCT
ACAGTCGGAGCGCATTGAGAAGCTAGGAGCCAAGCCACTTAGAGACCTCATTGACAAGATCGGTGGTTGG
AACATAACGGGGCCTTGGGACGAGGACAGCTTCATGGATGTGCTCAAGGCAGTCGCAGGGACCTACAGAG
CCACCCCCTTCTTCACCGTCTACGTCAGTGCTGATTCTAAGAGTTCTAACAGCAATATCATCCAGGTGGA
CCAGTCTGGGCTTTTTCTGCCCTCTCGAGATTACTACCTAAATAGAACTGCCAATGAGAAAGTTCTCACT
GCCTACCTGGACTACATGGTGGAGCTGGGAGTGCTGCTGGGTGGACAGCCGACCTCCACTCGGGAGCAGA
TGCAGCAGGTGCTGGAGCTGGAGATACAGCTGGCTAACATCACTGTGCCCCAGGACCAGCGGCGTGATGA
GGAGAAGATCTATCACAAGATGAGCATCTCAGAGCTGCAGGCTCTCGCGCCCGCCGTGGACTGGCTGGAG
TTCCTTTCTTTCTTGTTATCGCCACTTGAGTTGGGTGATTCTGAGCCTGTGGTGGTGTATGGGACTGAGT
ATTTACAGCAGGTGTCGGAGCTCATCAACCGTACTGAACCAAGCATCCTGAACAATTACCTAATTTGGAA
CCTGGTACAGAAGACGACCTCAAGCCTTGACCAGCGCTTTGAGACTGCACAGGAGAAACTGCTGGAGACC
CTCTACGGTACCAAGAAGTCCTGCACTCCGAGGTGGCAGACCTGCATCTCCAATACAGATGATGCCCTTG
GCTTTGCTCTGGGTTCACTCTTTGTGAAAGCCACATTTGACCGACAAAGCAAGGAAATCGCCGAGGGGAT
GATCAATGAAATCCGCTCTGCTTTTGAGGAGACCCTGGGAGACTTGGTTTGGATGGATGAGAAGACCCGG
CTGGCAGCCAAGGAGAAAGCAGATGCCATCTATGATATGATTGGTTTCCCTGATTTCATCCTGGAGCCCA
AAGAGCTGGATGATGTTTATGATGGGTATGAAGTCTCTGAAGATTCTTTTTTCCAAAACATGTTGAATCT
GTACAACTTCTCAGCTAAGGTGATGGCTGACCAGCTCCGCAAACCTCCCAGCCGAGACCAGTGGAGCATG
ACACCTCAGACCGTGAACGCTTACTACCTTCCAACCAAGAATGAAATCGTCTTCCCTGCTGGCATCTTGC
AGGCCCCCTTCTATGCTCACAACCATCCAAAGGCCTTGAACTTTGGTGGCATCGGCGTAGTGATGGGCCA
TGAGTTGACACATGCCTTTGATGACCAAGGGCGTGAGTATGACAAAGAAGGGAATCTGCGTCCTTGGTGG
CAGAATGAGTCACTGACGGCTTTCCAGAACCATACAGCCTGCATGGAAGAACAGTACAGCCAGTACCAGG
TCAATGGAGAGAGGCTCAATGGACTCCAGACCCTGGGGGAAAACATCGCCGATAATGGGGGCCTTAAGGC
TGCTTACAATGCTTACAAAGCATGGCTGAGAAAGCATGGGGAGGAGCAGCCGCTGCCTGCTGTGGGGCTC
ACCAATCACCAGCTTTTCTTCGTGGGATTTGCTCAGGTGTGGTGCTCGGTCCGCACACCAGAGAGCTCTC
ACGAGGGGCTGGTGACCGACCCCCACAGCCCTGCCCGTTTCCGAGTGCTGGGCACTCTCTCCAACTCCCG
AGACTTCCTTCGGCACTTCGGCTGCCCTGTCGGCTCCCCCATGAACCCAGGGCAGCTATGTGAGGTGTGG
TAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_177941
Insert Size 2733 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177941.1, NP_808810.1
RefSeq Size 3436 bp
RefSeq ORF 2733 bp
Locus ID 110599584
UniProt ID P0DPE0
Cytogenetics 16 B1
Summary This locus represents naturally occurring readthrough transcription between the adjacent genes eukaryotic translation elongation factor 1 alpha lysine specific methyltransferase 4 (GeneID:110599566) and endothelin converting enzyme 2 (GeneID:107522). The readthrough transcript representing this gene encodes a fusion protein that shares sequence identity with each individual gene product. provided by RefSeq, Jul 2017
Transcript Variant: This variant (1, also known as 2a-2) represents the longer transcript and encodes the longer isoform (a, also known as 2a-2).
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Documents
Resources
"NM_177941" in other vectors (4)
SKU Description Size Price
MG219025 Ece2 (tGFP-tagged) - Mouse endothelin converting enzyme 2 (Ece2) transcript variant 1, (10ug) 10 ug
$1,033.00
MR219025 Ece2 (Myc-DDK-tagged) - Mouse endothelin converting enzyme 2 (Ece2), transcript variant 1 10 ug
$589.00 $833.00
MR219025L3 Lenti ORF clone of Ece2 (Myc-DDK-tagged) - Mouse endothelin converting enzyme 2 (Ece2), transcript variant 1 10 ug
$1,133.00
MR219025L4 Lenti ORF clone of Ece2 (mGFP-tagged) - Mouse endothelin converting enzyme 2 (Ece2), transcript variant 1 10 ug
$1,133.00

file-alt Citations

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