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Piwil1 (NM_021311) Mouse Untagged Clone

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SKU
MC222266
Piwil1 (untagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $790.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Piwil1
Synonyms MIWI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC222266 representing NM_021311
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGGCCGAGCCCGAGCTCGGGCCCGCGGCAGGGCACGAGGTCAGGAGACGGTGCAGCATGTTGGGG
CTGCTGCGAGCCAGCAACCTGGGTACATCCCACCGAGACCTCAACAGTCCCCCACAGAGGGGGACTTGGT
TGGCCGAGGACGACAGAGGGGGATGGTAGTCGGAGCCACATCCAAGTCACAAGAACTGCAGATCTCAGCT
GGGTTTCAGGAGCTGTCACTGGCAGAGAGAGGAGGGCGTCGCCGAGACTTCCATGACCTTGGTGTGAACA
CCAGACAGAACCTTGACCATGTCAAAGAGTCAAAGACAGGCTCCTCTGGCATCATTGTGAAGCTGAGCAC
CAACCACTTCCGGCTGACCTCGCGCCCACAGTGGGCCCTGTATCAGTACCACATCGACTACAATCCCCTG
ATGGAGGCCCGAAGGCTTCGCTCCGCACTGCTCTTCCAGCATGAAGACCTCATTGGAAGGTGTCATGCTT
TCGATGGGACAATATTGTTTTTACCTAAGAGACTACAGCACAAGGTCACAGAAGTATTCAGTCAGACTCG
GAATGGGGAACACGTGAGGATCACCATCACCCTGACCAACGAGCTGCCGCCCACCTCGCCCACCTGCCTG
CAGTTCTATAACATCATCTTCAGGAGGCTCTTGAAAATCATGAATTTGCAACAAATTGGACGGAATTATT
ACAATCCAAGTGACCCGATTGATATTCCAAACCACAGGTTGGTGATCTGGCCCGGCTTCACCACCTCCAT
CCTTCAGTATGAGAACAACATCATGCTCTGCACAGACGTCAGCCACAAGGTGCTCCGCAGCGAGACTGTC
CTAGACTTCATGTTCAATCTATACCAGCAGACAGAGGAGCACAAGTTCCAGGAGCAAGTGTCGAAGGAGC
TCATAGGCCTCATCGTTCTCACCAAGTACAATAACAAGACCTACCGGGTGGATGACATTGACTGGGACCA
GAATCCAAAGAGCACCTTCAAGAAGGCGGATGGCTCGGAGGTCAGCTTCCTGGAGTACTACAGGAAGCAA
TACAACCAGGAGATCACGGACCTGAAGCAGCCGGTGCTGGTGAGCCAACCCAAGCGGAGGAGAGGCCCCG
GCGGCACCCTGCCTGGCCCAGCTATGCTCATCCCTGAACTCTGCTATCTCACAGGCCTGACTGATAAAAT
GCGCAATGATTTCAATGTGATGAAGGACCTGGCAGTGCACACGCGGCTGACCCCTGAGCAGCGGCAGCGG
GAGGTGGGCCGCCTCATCGACTACATCCACAAGGATGACAATGTGCAGAGAGAGCTTCGAGACTGGGGCC
TGAGCTTCGACTCAAACTTGCTGTCCTTCTCTGGAAGAATCTTACAATCTGAGAAGATCCACCAGGGCGG
AAAGACGTTTGATTACAACCCACAATTTGCAGACTGGTCCAAAGAAACAAGAGGCGCGCCGCTGATCAGC
GTGAAGCCATTGGATAACTGGCTGCTGATCTATACCCGCAGGAATTATGAAGCAGCCAACTCACTGATAC
AGAACCTGTTCAAAGTGACTCCAGCCATGGGCATCCAGATGAAAAAGGCAATCATGATCGAGGTGGATGA
CAGAACAGAAGCTTATCTGAGAGCCTTGCAGCAGAAGGTGACGTCAGACACTCAGATAGTTGTCTGTCTC
TTGTCAAGTAATCGGAAGGACAAATATGATGCCATCAAGAAGTACTTGTGTACAGACTGCCCCACCCCAA
GTCAGTGTGTGGTGGCCCGGACCCTGGGCAAGCAGCAAACAGTCATGGCCATTGCCACCAAGATCGCCCT
GCAGATGAACTGCAAGATGGGAGGCGAGCTCTGGCGGGTGGACATGCCCCTGAAACTGGCAATGATCGTG
GGCATCGACTGTTACCATGACACCACAGCTGGGCGGAGGTCCATCGCAGGATTCGTCGCCAGCATCAATG
AAGGGATGACCCGCTGGTTCTCCCGCTGCGTCTTTCAGGACCGCGGGCAGGAGCTGGTGGATGGTCTCAA
GGTGTGCTTGCAAGCTGCTCTGAGGGCTTGGAGTGGCTGCAATGAATACATGCCCAGCCGTGTCATCGTG
TACCGAGACGGTGTGGGGGACGGGCAGCTGAAGACCCTGGTCAATTATGAGGTCCCACAGTTCCTAGATT
GCCTCAAGTCAGTCGGGAGAGGTTACAACCCAAGACTGACTGTAATCGTGGTGAAGAAGCGTGTCAATGC
CAGGTTTTTTGCTCAGTCTGGGGGAAGACTTCAGAACCCTCTTCCAGGGACAGTCATCGATGTGGAAGTC
ACCAGACCAGAGTGGTATGACTTTTTCATCGTGAGCCAGGCAGTGAGAAGCGGGAGTGTGTCCCCAACAC
ACTACAATGTCATCTATGACAGCAGTGGCCTGAAGCCCGACCACATCCAGCGGCTGACATACAAGCTCTG
CCACGTGTACTATAATTGGCCTGGAGTCATCCGAGTCCCTGCACCTTGCCAGTATGCACACAAGCTGGCC
TTCCTCGTGGGCCAGAGCATCCACAGAGAGCCAAACCTCTCCCTGTCCAACCGCCTCTACTACCTCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_021311
Insert Size 2589 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC129857, AAI29858
RefSeq Size 2909 bp
RefSeq ORF 2589 bp
Locus ID 57749
UniProt ID Q9JMB7
Cytogenetics 5 67.86 cM
Summary Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:11578866, PubMed:22121019, PubMed:21237665). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:11578866, PubMed:22121019, PubMed:21237665). Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:11578866, PubMed:22121019, PubMed:21237665). Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias) (PubMed:24757166). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle (PubMed:22121019). Acts as an endoribonuclease that cleaves transposon messenger RNAs (PubMed:22121019). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (PubMed:16938833). Probable component of some RISC complex, which mediates RNA cleavage and translational silencing (PubMed:16938833). Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (PubMed:16787948). Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1 (PubMed:28552346).UniProtKB/Swiss-Prot Function
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"NM_021311" in other vectors (4)
SKU Description Size Price
MG222848 Piwil1 (tGFP-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1), (10ug) 10 ug
$990.00
MR222848 Piwil1 (Myc-DDK-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1) 10 ug
$589.00 $790.00
MR222848L3 Lenti ORF clone of Piwil1 (Myc-DDK-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1) 10 ug
$1,090.00
MR222848L4 Lenti ORF clone of Piwil1 (mGFP-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1) 10 ug
$1,090.00

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