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Top1 (NM_009408) Mouse Untagged Clone

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SKU
MC221438
Top1 (untagged) - Mouse topoisomerase (DNA) I (Top1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $773.00
5 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Top1
Synonyms AI467334; D130064I21Rik; Top-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC221438 representing NM_009408
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGGGGACCATCTCCACAACGATTCCCAGATCGAAGCGGATTTCCGATTGAATGATTCTCACAAAC
ACAAAGATAAACACAAAGATCGAGAACACCGGCATAAGGAGCACAAGAAGGATAAGGATAAGGACCGGGA
AAAGTCTAAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACACAAAGAGAAAGAGAAAACCAAA
CACAAAGATGGCAGCTCAGAAAAACATAAAGACAAACATAAAGACAGAGACAAGGAAAGACGAAAGGAGG
AAAAGATTAGAGCTGCTGGGGATGCAAAAATAAAGAAGGAAAAGGAAAATGGCTTCTCTAGTCCGCCACG
AATTAAAGATGAACCTGAGGATGATGGCTATTTTGCTCCTCCAAAAGAGGATATTAAGCCATTGAAGAGG
CTTCGAGATGAAGATGATGCTGATTATAAACCTAAGAAAATTAAGACAGAAGATATCAAGAAGGAGAAGA
AAAGAAAATCAGAAGAGGAAGAGGATGGTAAACTTAAAAAGCCCAAGAATAAAGATAAAGATAAAAAAGT
TGCTGAGCCAGATAATAAGAAAAAGAAGCCGAAAAAGGAAGAGGAACAGAAGTGGAAATGGTGGGAAGAA
GAACGTTATCCAGAAGGCATCAAATGGAAATTCCTAGAGCATAAAGGGCCTGTCTTCGCTCCACCATATG
AGCCTCTGCCAGAGAGTGTCAAGTTTTACTATGATGGTAAAGTTATGAAGCTGAGTCCTAAAGCAGAAGA
AGTAGCTACATTCTTTGCAAAAATGCTTGACCACGAATATACTACTAAGGAAATATTCAGGAAAAATTTC
TTTAAAGATTGGAGAAAGGAAATGACTAATGACGAAAAAAATACGATTACCAACCTAAGCAAATGCGACT
TTACACAGATGAGCCAGTATTTCAAAGCGCAGTCAGAAGCTCGGAAACAGATGAGCAAGGAAGAAAAATT
GAAAATCAAAGAAGAAAATGAAAAGTTATTGAAAGAATACGGCTTTTGTGTAATGGATAATCATAGAGAG
CGGATTGCCAACTTCAAGATAGAGCCTCCGGGGCTTTTCCGAGGCCGAGGGAACCACCCCAAGATGGGTA
TGCTGAAGAGAAGGATCATGCCTGAGGACATCATCATCAACTGTAGCAAAGACGCAAAGGTTCCTTCTCC
CCCTCCTGGACATAAATGGAAGGAAGTCCGACATGATAACAAGGTTACTTGGCTGGTCTCCTGGACAGAG
AATATCCAAGGTTCTATCAAATATATCATGCTGAATCCCAGTTCACGAATCAAGGGTGAGAAAGACTGGC
AGAAGTATGAGACTGCGCGGCGTCTGAAGAAGTGTGTGGACAAGATCCGAAATCAGTATCGGGAAGACTG
GAAGTCCAAAGAGATGAAAGTTCGTCAGAGAGCTGTAGCACTGTACTTCATTGATAAGCTTGCTCTGAGA
GCAGGCAATGAGAAGGAAGAAGGAGAGACGGCAGACACTGTGGGCTGCTGTTCACTTAGAGTGGAACACA
TCAATTTACACCCAGAATTGGATGGTCAGGAGTATGTGGTGGAATTTGACTTTCCTGGGAAGGACTCAAT
CAGATACTATAACAAAGTCCCAGTTGAGAAACGAGTTTTTAAAAACTTACAACTGTTTATGGAGAACAAG
CAGCCTGAGGACGATCTTTTTGATCGACTTAATACTGGTATTCTAAATAAACATCTTCAGGATCTCATGG
AGGGCTTGACTGCCAAGGTGTTCCGTACCTACAATGCCTCCATCACACTACAGCAGCAGCTTAAAGAGCT
CACAGCCCCTGATGAGAATGTACCAGCGAAGATTCTATCTTATAACCGTGCCAATCGAGCTGTTGCAATT
CTTTGTAACCACCAGAGGGCGCCACCAAAGACCTTTGAGAAGTCAATGATGAACTTGCAGTCTAAGATTG
ATGCCAAGAAAGATCAGTTAGCAGATGCTCGAAGGGACCTGAAAAGTGCTAAGGCTGATGCCAAAGTCAT
GAAGGATGCAAAGACCAAGAAGGTAGTAGAGTCAAAAAAGAAGGCTGTACAGAGACTAGAAGAGCAGTTG
ATGAAGCTGGAAGTTCAAGCCACAGATCGAGAGGAGAACAAACAAATTGCCTTGGGGACCTCCAAACTCA
ATTATCTGGACCCTAGGATCACAGTGGCTTGGTGCAAAAAGTGGGGGGTCCCAATTGAGAAGATTTACAA
CAAAACCCAGAGGGAGAAGTTTGCTTGGGCCATTGATATGACTGATGAGGACTACGAGTTTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_009408
Insert Size 2304 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009408.2, NP_033434.2
RefSeq Size 3859 bp
RefSeq ORF 2304 bp
Locus ID 21969
UniProt ID Q04750
Cytogenetics 2 80.96 cM
Summary Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.UniProtKB/Swiss-Prot Function
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"NM_009408" in other vectors (4)
SKU Description Size Price
MG218547 Top1 (tGFP-tagged) - Mouse topoisomerase (DNA) I (Top1), (10ug). 10 ug
$1,253.00
MR218547 Top1 (Myc-DDK-tagged) - Mouse topoisomerase (DNA) I (Top1). Note: ORF is codon optimized 10 ug
$1,053.00
MR218547L3 Lenti ORF clone of Top1 (Myc-DDK-tagged) - Mouse topoisomerase (DNA) I (Top1). Note: ORF is codon optimized 10 ug
$1,353.00
MR218547L4 Lenti ORF clone of Top1 (mGFP-tagged) - Mouse topoisomerase (DNA) I (Top1). Note: ORF is codon optimized 10 ug
$1,353.00

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