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Large2 (NM_172670) Mouse Untagged Clone

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SKU
MC220532
Gyltl1b (untagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $696.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Large2
Synonyms 5730485C17Rik; AI891893; Gyltl1b; Largel; mKIAA4105
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC220532 representing NM_172670
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCCCGAGGTCGCCCCCGGGCAATGGGGGCCGCCGTGCTGCTGCTGCTGCTGTTGCTAGTGGTTG
GCTTCTTCCTGTTCGGCCGGGACCCGGATTATGGACTGGGCACAACTGCTACCCTCGATGAAGACCCGTA
CAGGAGTCGCAACCTCTCCGCCTCCAGCCCGCAGCTTCTACTGCCACCCAAGTGCGAGATGTTGCATGTG
GCTATCGTGTGTGCGGGATACAACTCCAGCCGAGAGATTATTACCCTAACGAAGTCCCTGCTATTCTACA
GGAAAAATCCGCTGCACCTCCACCTGATAACTGATGCCGTAGCCAGAAACATCCTGGAGACACTCTTCCG
AACATGGATGGTGCCAGCGGTGGTGGTCAGCTTCTATGATGCGGAAGAACTCAAGCCCCTGGTCTCCTGG
ATCCCCAACAAACACTACTCTGGCCTCTATGGGCTAATGAAGCTAGTACTTCCCAGCATCCTGCCTCCCA
GCCTGGCCCGAGTCATCGTCCTGGATACCGACGTCACTTTCTCCTCTGACATTGTGGAGCTCTGGGCACT
CTTTGATCATTTTTCTGACAAGCAGGTGGTCGGTCTCGTGGAGAACCAGAGCGACTGGTACCTGGGCAAC
CTCTGGAAGAACCATAGGCCCTGGCCTGCCTTGGGCAGGGGATTTAACACAGGTGTGATCCTGCTGTGGC
TGGACAGGCTCCAGCAAACTGGCTGGGAGCAGATGTGGAAGGTGACAGCCAAACGAGAGCTGCTCACTCT
GATGGCTACTTCCTTGGCTGACCAGGACATCTTCAATGCGGTCATCAAGGAGCACCCCCATCTGGTGCAC
CCCCTGCCCTGTGTCTGGAACGTGCAGCTGTCAGACCACACTCGGGCTGAGCGCTGCTACCTGGAAGCAG
CTGACCTCAAAGTGATCCACTGGAATTCACCAAAGAAGCTTCGAGTGAAGAACAAGCACGCAGAATTCTT
CCGTAATCTGCACTTGACCTTTCTGGGGTATGATGGGAAGCTACTGCGAAGAGAGCTCTTTGGATGCCCC
AACCAGTTCCCTCCTGGGGCCGAGCAGTTGCAACAGGCCCTAACACAGCTGGATGAGGAAGAGCCCTGCT
TTGAGTTCCGCCAACAGCAGCTCACTGTGCACCGGGTGCACATCACCTTCCTGCCCCACCAGCCGCCACC
TCCCCAGCCTCACGATGTCACCTTGGTGGCCCAACTCTCTATGGACCGGCTGCAGATGCTGGAAGCCCTG
TGCAGGCACTGGCCAGGCCCCATGAGCCTGGCCTTGTACCTGACAGATGAAGAGGCTCAACAATTTCTTC
ATTTTGTGGAAACGTCGCCAGTGCTCTCTATGAGGAAGGATGTGGCCTACCATGTAGTGTACCGGGACGG
TCCACTCTATCCAGTCAACCAGCTCCGCAACGTGGCCTTGGCCCAGGCTCTCACACCCTACGTCTTCCTC
AGTGATATTGACTTCTTACCTGCCTACTCCCTCTACGACTACCTCAGGGCTTCTATCGAGCAGCTGGAGC
TGGACAGTCGGCGCAAGACTGCTTTGGTGGTGCCTGCATTTGAGACCCTACACTACCGGTTCAGCTTCCC
AAACTCTAAGGCAGAGCTGTTGACGTTACTGGATGCCGGCTCCCTTCACACCTTTAGGTACCACGAGTGG
CCACAGGGTCACTCATCCACAGACTATTCCCGCTGGCGGGAAGCCCAGGCACCATACAGTGTGCAGTGGT
CAGCTGACTATGAACCCTACGTGGTGGTACCCCGTGACTGCCCCCGTTATGATCCTCGCTTTGTGGGATT
TGGCTGGAACAAGGTGGCCCACATCATAGAGTTGGATGCTCAGGAATATGAATTCCTGGTACTTCCTGAG
GCCTTCTCTATCCACTTGCCCCACGCTCCAAGTCTTGACATCTCCCGCTTCCGCTCCAGCCCCACCTACC
GCAACTGTCTCCAGGCCCTCAAGGAAGAGTTCCACCAGGACTTGTCAAGGCGCTATGGGTCTGCAGCCCT
GAAATACCTCACTGCCCTGCAGCAGGCCCGAAGTCGGGCCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_172670
Insert Size 2073 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172670.3, NP_766258.2
RefSeq Size 2453 bp
RefSeq ORF 2073 bp
Locus ID 228366
UniProt ID Q5XPT3
Cytogenetics 2 E1
Summary Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units -3-Xylose-alpha-1,3-GlcA-beta-1- to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE (PubMed:15958417).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes isoform 1. CCDS Note: The coding region has been updated to represent an alternate exon combination and to extend the N-terminus to one that is more supported by available conservation data.
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Documents
Resources
"NM_172670" in other vectors (3)
SKU Description Size Price
MR210059 Gyltl1b (Myc-DDK-tagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1 10 ug
$589.00 $632.00
MR210059L3 Lenti ORF clone of Gyltl1b (Myc-DDK-tagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1 10 ug
$932.00
MR210059L4 Lenti ORF clone of Gyltl1b (mGFP-tagged) - Mouse glycosyltransferase-like 1B (Gyltl1b), transcript variant 1 10 ug
$932.00

file-alt Citations

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