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Spag16 (NM_029160) Mouse Untagged Clone

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SKU
MC219998
Spag16 (untagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $655.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Spag16
Synonyms 4921511D23Rik; 4930524F24Rik; 4930585K05Rik; AV261009; Pf20; Wdr29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC219998 representing NM_029160
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTCCGTCTGGGGTCCCGCCGCTGCGGGTGCTGGAGGAGCTGGGCATTGGCTTGTCACCCACCG
GCGAGGTGACAGAGGCGGTGACCTCCGAGGGCGCCTACTACCTGGAGCAGGTCACCATAACGGAAACATC
GGAAGATGAATGCGAATATGAAGAGATACCAGATGACAATTTCAGCATTCCTGAAGGAGAAGAAGATCTG
GAAAAAGCAATTCACATCATTGGAGAGCAAGCCAGAGACATCCATATTTTGGAACAACAAACCATTCTTC
CTGCAAGGAATGTAATGCAGGAAGCCATAGAAGATTTTCTCTGCAATTTCCTGATCAAAATGGGGATGAC
CAGAACTCTGGACTGCTTCCAGGCCGAGTGGTATGAGTTAATACAGAAGAAAGGAAGTGACTTTAAAGGG
CTCGGTAATGTTCCCGATGTCTACAGCCAGGTGATGCTTTTAGAGACTGAGAACAAAAACTTGAAGAAGG
AATTGAAGCACTTCAAACAGGCAGCTGAAAAGGCCAAAGAAGATCTACTGAGAACTCAGAAGGAACGGGA
CTTCCATCGCATGCATCATAAGCGCATAGTGCAAGAAAAGAACAAGCTCATTGCTGACCTCAAAGGGCTG
AAATTGCATTATGCATCATACGAACCAACTATAAGGGTGTTACATGAGAAGCATCATGCTTTATTGAAGG
AGAAAATGCTGACCTCTTTGGAAAGAGATCGAGCTGTTGGGAAGATTTCTGGGCTTCAAGCAACACTGAA
GAACATAGACATGGGACATATTCAGGTTCCTGTAATTAAAGGATCTTATGAGAGTGCTTCTATCACAAGA
GAGTCAGGAGACAGAGCTGGTCACAGTTGTGAGAAAGAAAACAGTTCAGAGGGCCCAACTCAGAAAAGTC
TTCGTGAAGCCAGGGAAGAAGTTGGATATAAATCAAAGTTGAAGAATGAGAAAAAGGATTCAGAATTTCC
TGTAGATATGCAACCAGATCCAAACGTGACTTCATGTACAGAAAACGTTTCTGCAGCAAAATTTGACTAC
AAGCTAAACAATATTTTCAGACTTCATGAACTCCCAGTGAGCTGCATCGTCATGCATCCCTGCAGAGACT
ATCTCATCTCCTGTAGTGAAGATCGTCTCTGGAAGATGGTGGGCCTCCCACAGGGCAATGTACTTCTTAC
AGGATCTGGCCACACTGATTGGTTGTCTGGCTGCTGCTTCCATCCGAGTGGCAGCAAGTTGGCTACCTCA
AGTGGTGACAGCACAATCAAACTATGGGACCTCAACAAAGGTGAATGCACCTTGACCCTTGAAGGACACA
ACCATGCCGTATGGTCCTGCACCTGGCACTCCTGTGGTGACTTCGTGGCTTCTGCCTCACTGGATATGAC
GAGTAAAATCTGGGATGTCAACAGTGAACGGTGCAGATACACTTTGTATGGACACACAGACTCTGTGAAC
AGCATTGAGTTTTTCCCTTTCTCCAACATTCTTCTCACCGCTTCTGCAGACAAGACCTTGTCTGTGTGGG
ATGCACGAACAGGTAAATGTGAGCAGTCTCTGTATGGTCACATGCATTCTGTCAATGATGCCACCTTCAC
GCCTAGGGGTCACATAATAGCATCCTGTGATGCCCGTGGAGTTACAAAGCTGTGGGACTTCCGGAAACTC
ATTCCAATCGTGTCCATTGATGTGGGTCCAAGTTCGGGAAATGAGGTGAACTTTGACCAGTCAGGTCGAG
TTTTAGCTCAGGCGAGTGCCAACGGGATTATCCATTTGCTGGATCTTAAATCGGGGCAAATTCACAAACT
GGTGGGCCACGAGAGTGAAGTGCACAGTGTGGTGTTTTCCCACCTGGGGGAAAATCTGTATTCTGGAGGC
TCTGACGGCACCATTCGGTTGTGGATCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_029160
Insert Size 1920 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029160.3, NP_083436.2
RefSeq Size 2144 bp
RefSeq ORF 1920 bp
Locus ID 66722
UniProt ID Q8K450
Cytogenetics 1 C3
Summary Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (1; also known as Spag16L). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_029160" in other vectors (4)
SKU Description Size Price
MG222592 Spag16 (tGFP-tagged) - Mouse sperm associated antigen 16 (Spag16) transcript variant 1, (10ug) 10 ug
$795.00
MR222592 Spag16 (Myc-DDK-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 1 10 ug
$289.00 $595.00
MR222592L3 Lenti ORF clone of Spag16 (Myc-DDK-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 1 10 ug
$895.00
MR222592L4 Lenti ORF clone of Spag16 (mGFP-tagged) - Mouse sperm associated antigen 16 (Spag16), transcript variant 1 10 ug
$895.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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