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Cbfa2t3 (NM_009824) Mouse Untagged Clone

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SKU
MC219793
Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $636.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Cbfa2t3
Synonyms A630044F12Rik; AI465270; AW229127; Cbfa2t3h; ETO-2; Eto2; MTGR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC219793 representing NM_009824
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCAGGCATCCACCACCACTCTGGAGAGTGGGGCACTCCTCTCGGGACCCCGGGGTCTTCAGAATG
GAAGCCCAGCTCACAGGAAGGAGAAGGCTGCAGCAATGCCAGACTCCCCGGCTGAAGTGAAGACGCAGCC
CCGGTCCACACCCCCCAGCATGCCGCCCCCACCTCCCACCTCATCCCAGGGAGCCACACGACCACCCTCC
TTCACACCTCACACACATGGCGAGGATGGACCTGCGACGTCTCTTCCCCATGGCCGTTTCCACGGCTGCT
TAAAGTGGTCCATGGTCTGTCTCTTGATGAACGGCAGCAGCCACTCACCCACGGCCATCCACGGTGCCCC
ATCTACACCCAATGGCTTCAGCAACGGCCCAGCCACCTCATCCACGGCCTCGCTCTCCACACAGCACCTG
CCCCCGGCGTGCGGGGCACGGCAGCTCAGCAAGCTTAAGCGTTTCCTCACCACCCTACAGCAGTTTGGCA
GTGACATCTCACCTGAGATCGGGGAGCGCGTGCGCACACTGGTGCTGGGCCTGGTGAACTCAACTCTGAC
GATCGAAGAGTTTCATGCCAAGCTCCAGGAAGCCACCAACTTTCCACTGAGGCCGTTTGTTATCCCTTTT
CTGAAGGCTAATCTTCCACTGCTGCAGCGTGAGCTCCTGCACTGTGCCCGCCTGGCCAAACAGACACCTG
CCCAGTACCTGGCCCAGCACGAACAGCTGCTGCTGGACGCCAGCGCCACCTCCCCTGTCGACTCGTCTGA
GCTCCTGCTGGAAGTCAACGAGAACGGCAAAAGGAGAACACCTGACAGGACCAAAGAGAATGGATCAGAC
CGGGACCCTCTGCACCCCGACCACCTCAGTAAGCGGTCCTGCACCCTGAGCCCCGCCCAGCGCTGCAGCC
CCAGCAATGGGCTGCCCCACCCGACGCCACCCCCACCCCCGCACTATCGCCTGGAGGACATGGCCATGGC
CCACCATTTCCGGGACTCCTACCGCCATCCTGATCCCCGAGAGCTACGGGAACGCCACCGGCCCCTGGCC
ATACCTGGGTCTCGACAAGAAGAAGTGATTGATCACAGGCTCACAGAACGCGAGTGGGCAGAAGAATGGA
AGCACCTCAACAGTCTTCTGAACTGCATCATGGACATGGTGGAGAAGACCCGGCGATCCCTCACCGTCCT
GCGCCGGTGTCAGGAGGCCGACCGTGAGGAACTCAACCACTGGATCCGGTGCTACAGTGACTCTGAGGAG
GGGAAGAAGGGCCCTACCCCCATCTCTGCCCGGTCCCTCAACAGCTGCAGTGGCCCTGAGGGGTCTCAGC
TAGATGTTCACCGGGACTTCACGCCCAGGACCCTGTCTGGCTACATGCCTGAAGAGATCTGGAGGAAGGC
TGAAGAAGCTGTGAATGAGGTGAAGCGCCAGGCCATGTCAGAACTACAGAAAGCTGTGTCTGATGCGGAG
CGCAAAGCCCATGAACTCATCACCACAGAGCGTGCCAAGATGGAACGAGCCCTGGCGGAGGCCAAGCGAC
AGGCCTCGGAGGATGCCCTGACTGTCATCAACCAGCAAGAGGACTCCAGCGAGAGCTGCTGGAACTGCGG
GCGCAAGGCCAGCGAGACGTGCAGTGGCTGTAACGCCGCCCGCTACTGCGGGTCCTTCTGTCAGCACAAA
GACTGGGAGAAACACCATCACGTGTGCGGCCAGAGTCTGCAGGGCCCCGCGGCTGCAGTGGCTGACCCAC
TACCTGGACAGCCTGACGCCACTGCCAGCCCCAGCGAAGCCGGCTCGGCAGGGCCCTCTCGTCCCTGCTC
TCCGGGGCCGCCAGGCCCGCTGGACGCTGCTGTGCCCCGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_009824
Insert Size 1863 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009824.2, NP_033954.2
RefSeq Size 7659 bp
RefSeq ORF 1863 bp
Locus ID 12398
UniProt ID O54972
Cytogenetics 8 E1
Summary Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway. Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (By similarity). Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:16407974). Plays a role in granulocyte differentiation (PubMed:15231665).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.
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Documents
Resources
"NM_009824" in other vectors (4)
SKU Description Size Price
MG219692 Cbfa2t3 (tGFP-tagged) - Mouse core-binding factor runt domain alpha subunit 2 translocated to 3 (human) (Cbfa2t3) transcript variant 1, (10ug) 10 ug
$777.00
MR219692 Cbfa2t3 (Myc-DDK-tagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 1 10 ug
$289.00 $577.00
MR219692L3 Lenti ORF clone of Cbfa2t3 (Myc-DDK-tagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 1 10 ug
$877.00
MR219692L4 Lenti ORF clone of Cbfa2t3 (mGFP-tagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 1 10 ug
$877.00

file-alt Citations

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