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Cry1 (NM_007771) Mouse Untagged Clone

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SKU
MC219605
Cry1 (untagged) - Mouse cryptochrome 1 (photolyase-like) (Cry1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $565.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Cry1
Synonyms AU020726; AU021000; Phll1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_007771, the custom clone sequence may differ by one or more nucleotides


ATGGGGGTGAACGCCGTGCACTGGTTCCGAAAGGGACTCCGGCTCCACGACAACCCCGCCCTGAAGGAGT
GCATCCAGGGCGCCGACACCATCCGCTGCGTCTATATCCTCGACCCCTGGTTCGCCGGCTCTTCCAACGT
GGGCATCAACAGGTGGCGATTTTTGCTTCAGTGTCTTGAGGATCTTGATGCCAATCTACGAAAATTAAAT
TCTCGTCTGTTTGTGATTCGGGGACAGCCAGCTGATGTATTTCCCAGGCTTTTCAAGGAATGGAACATCA
CTAAACTCTCAATTGAGTATGATTCTGAGCCTTTTGGGAAGGAACGAGATGCAGCTATCAAGAAGCTGGC
TACTGAGGCTGGCGTGGAAGTCATCGTGCGCATTTCACATACACTGTATGACCTGGACAAGATCATAGAA
CTCAATGGCGGACAGCCACCTCTAACATATAAAAGGTTTCAGACTCTCGTCAGCAAGATGGAGCCACTGG
AGATGCCAGCAGACACCATCACATCAGATGTGATAGGAAAGTGCATGACCCCTCTGTCTGATGACCATGA
TGAGAAATATGGCGTTCCTTCCCTGGAAGAGCTCGGCTTTGATACAGATGGCCTGTCCTCTGCAGTGTGG
CCAGGAGGAGAAACTGAGGCACTTACACGTTTGGAAAGGCATTTGGAAAGAAAGGCCTGGGTGGCAAACT
TTGAACGACCTCGAATGAATGCAAACTCCCTGCTTGCAAGCCCAACTGGACTCAGTCCTTATCTCCGCTT
TGGTTGTTTATCATGTCGGCTGTTTTATTTCAAACTAACAGATCTCTACAAAAAGGTAAAGAAGAATAGT
TCCCCTCCCCTTTCTCTTTATGGGCAACTCCTGTGGCGTGAATTTTTTTATACAGCAGCCACAAACAACC
CACGCTTTGACAAAATGGAAGGGAACCCCATCTGTGTTCAGATCCCTTGGGACAAGAACCCCGAGGCTCT
GGCCAAATGGGCAGAAGGCCGGACAGGCTTCCCGTGGATTGACGCCATCATGACTCAGCTTCGTCAGGAG
GGCTGGATCCACCATTTAGCCAGACACGCGGTTGCCTGTTTCCTGACTCGTGGTGACCTGTGGATCAGCT
GGGAAGAAGGGATGAAGGTCTTTGAAGAGTTACTGCTTGATGCAGATTGGAGCATAAATGCTGGAAGTTG
GATGTGGCTGTCCTGCAGTTCCTTTTTTCAGCAATTTTTTCACTGCTACTGCCCTGTGGGTTTTGGTAGG
AGGACAGATCCCAATGGAGACTATATTAGGCGTTATTTACCTGTCCTAAGAGGCTTCCCTGCAAAATATA
TCTACGATCCTTGGAATGCACCAGAAGGCATCCAGAAGGTTGCCAAGTGTTTGATAGGAGTTAATTACCC
CAAACCGATGGTGAACCATGCTGAGGCAAGCAGACTGAATATTGAAAGAATGAAGCAGATCTATCAGCAG
CTTTCCCGGTACAGAGGGCTAGGTCTTCTCGCCTCGGTCCCTTCTAACTCTAATGGGAATGGAGGGCTCA
TGGGCTATGCTCCTGGAGAGAATGTCCCGAGTTGTAGCAGCAGCGGGAATGGAGGGCTCATGGGCTATGC
TCCTGGAGAGAACGTCCCGAGCTGTAGCGGTGGAAATTGCTCTCAAGGAAGTGGTATTTTACACTATGCT
CACGGGGACAGTCAGCAGACTCACTCACTCAAGCAAGGGAGAAGCTCCGCGGGCACCGGCCTCAGCAGTG
GGAAGCGTCCTAGTCAGGAAGAGGATGCCCAGAGTGTCGGCCCCAAAGTCCAGCGGCAGAGCAGTAACTG
A
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_007771
Insert Size 1821 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007771.3, NP_031797.1
RefSeq Size 3035 bp
RefSeq ORF 1821 bp
Locus ID 12952
UniProt ID P97784
Cytogenetics 10 41.63 cM
Summary This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of this gene results in a shortened circadian cycle in complete darkness. provided by RefSeq, Feb 2014
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"NM_007771" in other vectors (4)
SKU Description Size Price
MG225732 Cry1 (tGFP-tagged) - Mouse cryptochrome 1 (photolyase-like) (Cry1), (10ug) 10 ug
$489.00 $764.00
MR225732 Cry1 (Myc-DDK-tagged) - Mouse cryptochrome 1 (photolyase-like) (Cry1) 10 ug
$289.00 $564.00
MR225732L3 Lenti ORF clone of Cry1 (Myc-DDK-tagged) - Mouse cryptochrome 1 (photolyase-like) (Cry1) 10 ug
$864.00
MR225732L4 Lenti ORF clone of Cry1 (mGFP-tagged) - Mouse cryptochrome 1 (photolyase-like) (Cry1) 10 ug
$864.00

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