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Clgn (NM_009904) Mouse Untagged Clone

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SKU
MC219604
Clgn (untagged) - Mouse calmegin (Clgn), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $854.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Clgn
Synonyms 4930459O04Rik; A2/; A2/6; AI528775; C; calne; Cln
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC219604 representing NM_009904
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTTTCCAGGGTGTTGGACTATGTTTGGGTCTCCTGTTCATCACGGTCAATGCGGACTTTATGGATG
ATGGTGTTGAGGTGGAAGACTTCAGTGAAAATTCAGACGAGAGTAATATTAAAGATGAACCTTCCTCGGG
GACTTTTAAGTATAAGACACCTCAACCTATAGGAGAAGTATATTTTACAGAAACATTTGATAGTGGAAAT
CTGGCTGGGTGGGTCTTATCAAAAGCAAAGAAGGATGATATGGATTCAGAAATTGCTATATATGATGGAA
GATGGGAAATAGAAGAACTGAAGGAAAACCAGGTGCCTGGCGACAGAGGGCTGGTTCTGAAGTCTAAAGC
CAAGCACCACGCAATAGCCGCTGTCCTCGAAAAACCCTTTATTTTTGCCGATAAACCTTTGATAGTTCAA
TATGAAGTAAATTTTCAAGATGGTATTGATTGTGGAGGTGCATACATTAAACTGCTAGCAGACACTGGTG
ATTTGATTCTGGAAAACTTTTATGATAAAACATCCTATACCATCATGTTTGGACCAGATAAATGTGGAGA
GGATTATAAACTTCATTTAATCTTCCGACACAAACATCCGAAAACTGGAGTTTTTGAAGAAAAGCATGCC
AAGCCTCCAGATGTCGATCTGAAGGAGTTCTTTACAGACAGGAAGACTCACCTCTATACCCTTGTGATGA
ATCCGGATGATACATTTGAAGTATTAATTGATCAGAAAGTTGTGAACCAAGGGACCTTGCTAGATGATGT
GGTCCCGCCTATCAACCCACCCAGAGAAATTGACGACCCCAGTGATAAAAAGCCAGAGGAGTGGGATGAC
AGAGCCAAGATCCCCGACCCTACCGCCGTCAGGCCAGAGGACTGGGATGAAAATGAACCTGCCCAAATAG
AAGACTCAAGTGCTGTTAAACCTGATGGCTGGCTTGATGATGAACCAAAGTTTATCCCCAATCCTAAAGC
TGAGAAACCTGAGGACTGGAGTGACGACATGGATGGAGAATGGGAGGCACCACATATTCCTAATCCAGCC
TGTCAGATTGGATGTGGAGAATGGAAACCTCCCATGATCGATAACCCAAAATACAAAGGAATCTGGAGAC
CACCAATGATCAATAATCCTAACTACCAGGGACTCTGGAGTCCTCAGAAGATTCCCAACCCAGATTACTT
TGAGGATGACCACCCCTTCCTCCTGACTTCTTTCAGTGCGCTGGGCTTAGAGCTTTGGTCCATGACTCCT
GACATCTACTTTGATAATTTTATCATCTGTTCAGAAAAGGAGGTGGCCGATCAGTGGGCTACAGATGGCT
GGGAACTGAAGATAATGGTGGCAAATGCTAATGAGCCTGGTGTACTCCGACAGCTGGTGATAGCCGCAGA
GGAGCGCCCCTGGCTCTGGCTCATGTACCTGGTGATGGCCGGCTTGCCCGTTGCATTAGTTGCTTCCTTT
TGTTGGCCAAGGAAAGTAAAGAAAAAATATGAAGACACGGGACCTAAAAAAACTGAATTATGCAAACTGC
AAAGCAAGGCCGCTCTGGAACAAGAGGCAGAGGAGGAGAAGGCCCCAGAGAAGCCAGAAGATGTGCAAGA
AGAAAAGAAGCCAGGTGAAGCTGAGGTCGTCACCGTGGAAAAGGAAGTAATTGGTGAACCGGAAGAGAAG
AGTAAGGAAGACAGAGAAACTCTAGAAGGTCAAGAGGAAGTGAGCAAGCTGAGTAAATCTGGATCAGAGG
ATGAGATGAAGGACGCTGATGAGAGCCCAGGATCTGGAGATGCCCCACTGAAGTCATTGCGGAAGAGGAG
GGTACGGAAGGATTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_009904
Insert Size 1836 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009904.3, NP_034034.2
RefSeq Size 2307 bp
RefSeq ORF 1836 bp
Locus ID 12745
UniProt ID P52194
Cytogenetics 8 39.97 cM
Summary This gene belongs to the calreticulin family, which includes calreticulin, calnexin, and calmegin, and encodes a calcium-binding molecular chaperone specifically expressed in pachytene stage male germ cells. It is required for the proper folding of newly synthesized membrane proteins in the endoplasmic reticulum including those critical for sperm migration from the uterus into the oviduct and sperm adhesion to and penetration of the zona pellucida. This gene plays a key role in spermatogenesis and male infertility. Alternative splice variants exist for this gene. provided by RefSeq, Jul 2016
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_009904" in other vectors (4)
SKU Description Size Price
MG209394 Clgn (tGFP-tagged) - Mouse calmegin (Clgn) 10 ug
$1,053.00
MR209394 Clgn (Myc-DDK-tagged) - Mouse calmegin (Clgn) 10 ug
$853.00
MR209394L3 Lenti ORF clone of Clgn (Myc-DDK-tagged) - Mouse calmegin (Clgn) 10 ug
$1,153.00
MR209394L4 Lenti ORF clone of Clgn (mGFP-tagged) - Mouse calmegin (Clgn) 10 ug
$1,153.00

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