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Gls (NM_001113383) Mouse Untagged Clone

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SKU
MC219497
Gls (untagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $618.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gls
Synonyms 6330442B14; AI314027; B230365M23Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC219497 representing NM_001113383
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCGGCTGCGAGGCTCGGCGATGCTACGGGAGCTGCTCTTAAGGCCGCCCGCCGCCGTCGGAGCCG
TCCTGCGGCGCGCGCAGCCCCTCGGCACGCTGTGCCGGCGCCCCCGGGGCGGGAGCCGGCCGACCGCTGG
CCTGGTGGCCGCTGCGCGACTCCACCCGTGGTGGGGCGGGGGCGGCCGCGCGAAGGGCCCCGGCGCGGGC
GGCCTGTCCAGTTCGCCCTCGGAGATCCTACAGGAGCTGGGGAAGGGGGGTACGCCGCCACAGCAGCAGC
AGCAGCAGCAGCAGCAGCCAGGGGCGTCGCCACCCGCAGCCCCGGGCCCCAAGGACAGCCCGGGGGAGAC
CGACGCGTTCGGCAACAGCGAGGGCAAGGAGATGGTGGCCGCGGGCGACAATAAAATAAAACAGGGTCTG
TTACCTAGCTTGGAAGATTTGCTGTTCTATACAATTGCAGAAGGACAAGAAAAGATACCTGTTCACAAGT
TTATTACAGCACTCAAATCTACAGGATTGCGAACATCTGATCCCAGGTTGAAAGAGTGTATGGATATGTT
AAGATTAACTCTTCAGACAACGTCAGATGGTGTCATGCTAGACAAAGATCTTTTTAAAAAGTGTGTTCAA
AGCAACATTGTTTTGTTGACACAAGCATTTAGAAGAAAGTTTGTCATTCCTGACTTTATGTCTTTTACCT
CACACATCGATGAGTTATATGAAAGTGCTAAAAAGCAGTCTGGAGGGAAGGTTGCTGATTATATTCCTCA
GCTGGCCAAATTCAGTCCTGATTTGTGGGGTGTATCTGTCTGTACTGTAGATGGGCAAAGGCATTCTATT
GGAGATACCAAAGTTCCTTTTTGTCTTCAGTCCTGTGTAAAACCCCTGAAATATGCAATTGCTGTTAATG
ACCTGGGAACTGAGTATGTACATCGCTATGTTGGGAAGGAGCCAAGTGGATTAAGATTCAACAAACTCTT
TTTGAATGAAGATGATAAACCACATAATCCTATGGTAAATGCTGGAGCAATTGTTGTGACTTCTCTAATA
AAGCAAGGAGTAAATAATGCTGAGAAGTTTGACTACGTGATGCAATTTTTGAATAAGATGGCTGGTAATG
AATATGTTGGATTCAGTAATGCAACGTTTCAGTCTGAACGAGAAAGTGGAGATCGAAATTTTGCAATAGG
ATATTACTTAAAAGAAAAGAAGTGTTTTCCAGAAGGCACAGACATGGTTGGGATACTAGATTTTTACTTC
CAGCTGTGCTCTATTGAAGTGACATGTGAATCAGCAAGTGTGATGGCTGCCACCTTGGCTAATGGTGGTT
TCTGCCCAATTACTGGTGAAAGAGTCCTCAGTCCTGAGGCAGTTCGGAATACACTGAGCTTGATGCATTC
TTGTGGCATGTATGACTTCTCAGGGCAGTTTGCGTTCCATGTTGGTCTTCCTGCAAAATCTGGAGTTGCT
GGGGGTATTCTTTTAGTTGTCCCCAACGTCATGGGCATGATGTGTTGGTCTCCTCCTCTTGACAAGATGG
GCAACAGTGTTAAGGGAATTCACTTTTGTCACGATCTTGTTTCTCTGTGTAACTTCCATAACTATGATAA
TTTGAGACACTTTGCAAAAAAACTTGATCCTCGGAGAGAAGGAGGTGATCAAAGGCATTCCTTTGGACCA
TTGGACTATGAGAGTCTCCAGCAAGAACTTGCTTTAAAAGACACAGTATGGAAAAAAGTGTCACCTGAGT
CAAGTGACGACACCTCTACAACTGTAGTATATAGAATGGAGAGTCTGGGGGAGAGGAGCTAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII |
ACCN NM_001113383
Insert Size 1812 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001113383.1, NP_001106854.1
RefSeq Size 4419 bp
RefSeq ORF 1812 bp
Locus ID 14660
UniProt ID D3Z7P3
Cytogenetics 1 26.86 cM
Summary Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) includes an alternate exon, compared to variant 1, resulting in a shorter protein (isoform 2) that has a distinct C-terminus, compared to isoform 1.
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Documents
Resources
"NM_001113383" in other vectors (6)
SKU Description Size Price
MG215212 Gls (tGFP-tagged) - Mouse glutaminase (Gls) nuclear gene encoding mitochondrial protein transcript variant 2, (10ug) 10 ug
$489.00 $762.00
MR215212 Gls (Myc-DDK-tagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$289.00 $562.00
MR215212L1 Lenti ORF clone of Gls (Myc-DDK-tagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$862.00
MR215212L2 Lenti ORF clone of Gls (mGFP-tagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$862.00
MR215212L3 Lenti ORF clone of Gls (Myc-DDK-tagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$862.00
MR215212L4 Lenti ORF clone of Gls (mGFP-tagged) - Mouse glutaminase (Gls), nuclear gene encoding mitochondrial protein, transcript variant 2 10 ug
$862.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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