Ikzf4 (NM_011772) Mouse Untagged Clone

SKU
MC219289
Ikzf4 (untagged) - Mouse IKAROS family zinc finger 4 (Ikzf4), (10ug)
  $819.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ikzf4
Synonyms A630026H08Rik; Eos; Zfpn1a4; Znfn1a4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC219289 representing NM_011772
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACACACCACCCGCACTCCCTCGCCGTTTCCAAGGCGGCGGCCGCGTTCGCACCCCAGGGTCTCACC
GGCAAGGGAAGGATAATCTGGAGAGGGAGCTCTCAGGAGGGTGTGCTCCGGATTTCTTGCCTCAGGCCCA
GGACTCCAACCATTTTATAATGGAATCTTTATTTTGTGAAAGTAGCGGGGACTCATCTCTGGAGAAGGAG
TTCCTTGGGGCCCCAGTGGGGCCCTCGGTGAGCACCCCAAACAGCCAACACTCTTCACCCAGCCGCTCGC
TCAGTGCCAACTCCATCAAGGTGGAGATGTACAGCGATGAGGAGTCGAGCAGACTGCTGGGGCCGGATGA
ACGGCTCCTGGATAAGGATGACAGTGTGATTGTGGAAGACTCATTGTCAGAGCCCTTAGGCTACTGCGAT
GGAAGTGGGCCAGAGCCTCACTCCCCTGGCGGCATCCGGCTACCCAACGGCAAGCTCAAGTGCGACGTCT
GCGGCATGGTCTGCATTGGGCCCAATGTGCTCATGGTACACAAGCGCAGCCACACTGGGGAGAGGCCCTT
CCACTGTAATCAGTGTGGTGCCTCCTTCACACAGAAGGGCAATCTGCTTCGCCACATCAAGCTGCACTCG
GGGGAGAAGCCCTTCAAGTGCCCCTTCTGCAACTATGCCTGCCGCCGGCGTGACGCACTCACTGGCCACC
TCCGCACACACTCAGTCTCCTCCCCCACCGTGGGCAAACCCTACAAGTGCAACTACTGTGGCCGGAGCTA
CAAACAGCAAAGTACCCTGGAGGAGCACAAGGAGAGGTGCCACAACTACCTACAGAGTCTCAGCACTGAT
GCCCAAGCTCTGACTGGCCAGCCAGGTGATGAAATCCGTGACCTGGAGATGGTGCCTGACTCAATGCTGC
ACCCATCGACTGAACGGCCAACTTTCATTGATCGTTTGGCCAACAGCCTCACCAAACGCAAGCGTTCCAC
CCCACAGAAGTTTGTAGGTGAAAAGCAGATGCGCTTCAGCCTCTCAGACCTTCCCTATGATGTGAATGCC
AGCGGTGGCTATGAAAAGGACGTAGAGTTGGTGGCACACCATGGCCTGGAGCCTGGCTTTGGAGGGTCTC
TAGCCTTTGTGGGTACAGAGCATCTGCGTCCCCTCCGCCTCCCACCCACCAACTGCATCTCAGAACTCAC
ACCTGTCATCAGCTCTGTGTACACCCAAATGCAGCCCATCCCCAGCCGACTGGAGCTTCCAGGGTCCCGA
GAAGCAGGTGAGGGACCGGAGGACCTGGGAGATGGAGGTCCCCTCCTTTATCGGGCCCGAGGCTCTCTGA
CTGACCCTGGGGCATCCCCCAGCAATGGCTGCCAGGACTCCACAGATACAGAGAGCAACCACGAAGACCG
GATTGGTGGGGTGGTATCCCTTCCTCAGGGTCCCCCACCCCAACCTCCTCCCACCATAGTGGTGGGCCGG
CACAGTCCCGCCTATGCCAAAGAGGACCCCAAACCACAGGAGGGGTTACTGCGGGGCACCCCAGGCCCCT
CCAAGGAAGTGCTTCGGGTGGTGGGTGAGAGTGGTGAGCCAGTGAAGGCCTTTAAGTGTGAACACTGCCG
CATCCTCTTTCTGGACCACGTCATGTTCACCATCCACATGGGCTGCCACGGCTTCAGAGACCCTTTTGAG
TGTAACATCTGTGGTTATCACAGCCAGGATCGGTATGAGTTCTCTTCCCACATCGTCCGGGGGGAACATA
AGGTGGGCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_011772
Insert Size 1761 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011772.2, NP_035902.2
RefSeq Size 3580 bp
RefSeq ORF 1761 bp
Locus ID 22781
UniProt ID Q8C208
Cytogenetics 10 D3
Summary DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3-mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1 (PubMed:19696312).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011772" in other vectors (4)
SKU Description Size Price
MG216195 Ikzf4 (tGFP-tagged) - Mouse IKAROS family zinc finger 4 (Ikzf4), (10ug) 10 ug
$1,018.00
MR216195 Ikzf4 (Myc-DDK-tagged) - Mouse IKAROS family zinc finger 4 (Ikzf4) 10 ug
$818.00
MR216195L3 Lenti ORF clone of Ikzf4 (Myc-DDK-tagged) - Mouse IKAROS family zinc finger 4 (Ikzf4) 10 ug
$1,118.00
MR216195L4 Lenti ORF clone of Ikzf4 (mGFP-tagged) - Mouse IKAROS family zinc finger 4 (Ikzf4) 10 ug
$1,118.00

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