Ebf2 (NM_010095) Mouse Untagged Clone

SKU
MC219158
Ebf2 (untagged) - Mouse early B-cell factor 2 (Ebf2), (10ug)
  $858.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ebf2
Synonyms D14Ggc1e; EBF-2; Mmot1; O/E-3; OE-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI |
ACCN NM_010095
Insert Size 1728 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010095.6, NP_034225.1
RefSeq Size 5527 bp
RefSeq ORF 1728 bp
Locus ID 13592
UniProt ID O08792
Cytogenetics 14 D1
Summary Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) uses an alternate splice site in 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.
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Resources
"NM_010095" in other vectors (4)
SKU Description Size Price
MG224591 Ebf2 (tGFP-tagged) - Mouse early B-cell factor 2 (Ebf2), (10ug) 10 ug
$1,056.00
MR224591 Ebf2 (Myc-DDK-tagged) - Mouse early B-cell factor 2 (Ebf2) 10 ug
$856.00
MR224591L3 Lenti ORF clone of Ebf2 (Myc-DDK-tagged) - Mouse early B-cell factor 2 (Ebf2) 10 ug
$1,156.00
MR224591L4 Lenti ORF clone of Ebf2 (mGFP-tagged) - Mouse early B-cell factor 2 (Ebf2) 10 ug
$1,156.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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