Gpi1 (NM_008155) Mouse Untagged Clone

CAT#: MC218919

Gpi1 (untagged) - Mouse glucose phosphate isomerase 1 (Gpi1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpi1
• Synonyms: AM; Amf; Gpi; Gpi-; Gpi-1; Gpi-1r; Gpi-1s; Gpi-1t; Gpi1-r; Gpi1-s; Gpi1-t; Gpi1s; MF; N; neuro; NK; NK/; NK/GPI; Nlk; O; Org; Pgi; Phi
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218919 representing NM_008155
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGCTCACCCGGAACCCGCAGTTCCAGAAGCTCCTGGAGTGGCACCGCGCGAACTCTGCCAACC
  TCAAGCTGCGCGAACTTTTTGAGGCGGATCCGGAGCGCTTCAACAACTTCAGCTTGAACCTCAACACCAA
  CCATGGGCATATTCTGGTGGACTACTCCAAGAACCTTGTGAACAAGGAGGTGATGCAGATGCTGGTGGAG
  CTGGCCAAGTCCAGAGGCGTGGAGGCTGCACGGGACAACATGTTCAGTGGTTCCAAGATCAACTACACCG
  AGAATCGGGCGGTGCTGCATGTGGCCCTTCGGAACCGGTCCAACACACCCATCAAGGTGGACGGCAAAGA
  TGTGATGCCGGAGGTGAACAGGGTTCTGGACAAGATGAAGTCTTTCTGCCAGCGGGTCCGGAGTGGTGAC
  TGGAAAGGGTACACTGGCAAATCCATCACGGACATCATCAACATCGGCATCGGGGGCTCTGACCTGGGAC
  CCCTCATGGTGACTGAAGCTCTCAAGCCTTACTCGAAAGGAGGTCCCCGTGTCTGGTTTGTCTCTAACAT
  TGATGGGACCCACATTGCCAAAACACTGGCCAGCTTGTCCCCTGAGACTTCCCTCTTTATAATCGCCTCC
  AAGACCTTCACCACCCAGGAGACCATCACCAATGCAGAGACAGCAAAGGAGTGGTTTCTCGAAGCGGCCA
  AGGATCCATCTGCAGTTGCAAAGCACTTTGTCGCCCTGTCTACGAACACGGCCAAAGTGAAAGAGTTTGG
  AATTGACCCTCAAAACATGTTCGAGTTCTGGGATTGGGTAGGTGGCCGCTATTCGCTGTGGTCAGCCATT
  GGACTTTCCATTGCTCTGCATGTAGGTTTTGACCACTTCGAGCAGCTGCTGTCCGGGGCTCACTGGATGG
  ACCAGCACTTCCTCAAGACGCCCCTGGAGAAGAATGCCCCCGTCCTGCTGGCTCTACTGGGCATCTGGTA
  CATCAACTGCTACGGCTGTGAGACCCACGCCTTGCTGCCCTATGACCAGTACATGCACCGCTTTGCTGCC
  TATTTCCAGCAGGGTGACATGGAGTCCAACGGAAAGTACATCACCAAGTCCGGGGCCCGTGTGGACCACC
  AGACAGGCCCCATCGTGTGGGGGGAACCAGGGACCAATGGTCAACATGCATTCTACCAGCTCATCCACCA
  AGGCACCAAGATGATACCCTGTGACTTTCTCATCCCTGTCCAGACCCAGCACCCCATACGGAAAGGTCTG
  CATCACAAGATCCTCCTGGCTAACTTCTTGGCCCAGACTGAGGCCCTGATGAAGGGGAAGTTGCCTGAAG
  AGGCCAGGAAGGAGCTCCAGGCTGCCGGAAAGAGCCCAGAAGACTTGGAGAAACTCTTGCCACACAAGGT
  CTTTGAAGGAAACCGCCCGACCAACTCTATTGTGTTTACCAAGCTGACACCCTTCATTCTGGGGGCCTTG
  ATTGCCATGTATGAGCACAAGATCTTTGTTCAGGGCATCATGTGGGACATCAACAGCTTCGACCAGTGGG
  GAGTGGAGCTGGGGAAGCAGCTGGCCAAGAAAATTGAGCCGGAGCTGGAGGGCAGCTCTGCTGTGACCTC
  CCATGATTCCTCCACTAACGGACTGATCAGCTTCATCAAGCAACAGCGGGACACCAAACTAGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008155
• Insert Size: 1677 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008155.4, NP_032181.2
• RefSeq Size: 2887 bp
• RefSeq ORF: 1677 bp
• Locus ID: 14751
• UniProt ID: P06745
• Cytogenetics: 7 19.46 cM
• Gene Summary: This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. [provided by RefSeq, Aug 2016]