Qk (NM_001159517) Mouse Untagged Clone

SKU
MC217738
Qk (untagged) - Mouse quaking (Qk), transcript variant 1, (10ug)
  $503.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Qk
Synonyms 1110003F05Rik; 1500005P18; l(17)-1Wis; l17Wis1; QkI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217738 representing NM_001159517
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCGGGGAAATGGAAACGAAGGAGAAGCCGAAGCCCACCCCAGATTATTTGATGCAGCTGATGAACG
ACAAGAAGCTCATGAGCAGCCTGCCCAACTTCTGCGGGATCTTCAACCACCTCGAGCGGCTGCTGGACGA
AGAAATTAGCAGAGTACGGAAAGACATGTACAATGACACGTTAAATGGCAGTACAGAGAAAAGAAGTGCA
GAATTGCCTGACGCGGTGGGACCCATTGTTCAGTTACAAGAGAAACTTTATGTGCCTGTAAAAGAATACC
CTGATTTTAATTTTGTTGGGAGAATCCTTGGACCTAGAGGACTTACAGCTAAACAACTTGAAGCAGAAAC
GGGATGTAAAATAATGGTCCGAGGCAAAGGCTCAATGAGGGATAAAAAGAAGGAGGAGCAAAATAGAGGC
AAGCCCAATTGGGAGCATCTAAATGAAGACTTACATGTACTAATCACTGTGGAAGATGCTCAGAACAGAG
CAGAAATCAAGCTGAAGAGAGCGGTTGAAGAAGTGAAGAAGTTACTGGTACCTGCGGCTGAAGGTGAAGA
CAGCCTGAAGAAGATGCAGCTGATGGAGCTTGCAATTCTGAATGGCACCTACAGAGACGCCAACATTAAA
TCACCAGCCCTTGCCTTTTCTCTTGCAGCAACTGCCCAGGCTGCTCCAAGGATCATCACTGGGCCTGCGC
CTGTCCTCCCACCAGCTGCTCTGCGTACACCTACGCCAGCTGGCCCTACCATAATGCCTTTGATCAGACA
AATACAGACCGCTGTCATGCCAAACGGAACTCCTCACCCAACTGCTGCAATAGTCCCTCCAGGGCCTGAA
GCTGGGTTAATCTACACACCCTATGAATACCCCTACACATTGGCACCAGCTACATCAATCCTTGAGTACC
CTATTGAACCCAGTGGTGTGTTAGGTGCGGTGGCTACTAAAGTTCGAAGGCACGATATGCGTGTCCATCC
TTACCAAAGGATTGTGACCGCAGACCGAGCCGCCACCGGCAACTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001159517
Insert Size 1026 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001159517.1, NP_001152989.1
RefSeq Size 4658 bp
RefSeq ORF 1026 bp
Locus ID 19317
UniProt ID Q9QYS9
Cytogenetics 17 7.75 cM
Summary RNA-binding protein that plays a central role in myelinization (PubMed:10864952, PubMed:11917126). Also required for visceral endoderm function and blood vessel development (PubMed:11892011, PubMed:16470614). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence (PubMed:16041388). Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation, as well as cellular processes including apoptosis, cell cycle, glial cell fate and development (PubMed:10535969, PubMed:12467586, PubMed:11297509, PubMed:11917126, PubMed:15568022). Required to protect and promote stability of mRNAs such as MBP and CDKN1B which promotes oligodendrocyte differentiation (PubMed:10535969, PubMed:15568022). Participates in mRNA transport by regulating the nuclear export of MBP mRNA (PubMed:12467586). May also play a role in smooth muscle development (PubMed:14706070).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_001159517" in other vectors (3)
SKU Description Size Price
MR205120 Qk (Myc-DDK-tagged) - Mouse quaking (Qk), transcript variant 1 10 ug
$289.00 $457.00
MR205120L3 Lenti ORF clone of Qk (Myc-DDK-tagged) - Mouse quaking (Qk), transcript variant 1 10 ug
$757.00
MR205120L4 Lenti ORF clone of Qk (mGFP-tagged) - Mouse quaking (Qk), transcript variant 1 10 ug
$757.00

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