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Dntt (NM_009345) Mouse Untagged Clone

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SKU
MC217581
Dntt (untagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $544.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dntt
Synonyms Tdt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217581 representing NM_009345
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCACTGCAAGCAGTCCACCTGGGTCCTCGGAAGAAAAGACCCAGGCAGCTGGGCACCCCAGTGG
CTTCCACGCCTTATGACATCCGATTTCGAGACTTGGTCCTCTTCATTTTGGAAAAGAAGATGGGAACAAC
TCGAAGAGCCTTCCTCATGGAGCTGGCCCGAAGGAAAGGGTTCAGGGTGGAAAATGAGCTCAGTGATTCG
GTCACCCACATTGTGGCAGAGAACAACTCAGGGTCAGACGTCCTGGAGTGGCTCCAGCTGCAGAACATCA
AAGCCAGCTCCGAGCTCGAACTCTTGGACATCTCCTGGCTCATCGAATGCATGGGAGCTGGGAAGCCAGT
GGAGATGATGGGGAGACATCAGCTTGTTGTGAATAGAAACTCCTCCCCGAGTCCTGTCCCAGGCTCCCAG
AATGTTCCAGCACCTGCTGTGAAGAAGATCTCCCAATATGCTTGCCAGCGAAGAACCACATTAAACAATT
ACAACCAACTATTCACGGATGCCCTTGATATCCTGGCTGAAAATGATGAGCTTAGAGAGAATGAAGGTTC
TTGCCTGGCATTCATGCGAGCGTCCTCTGTACTGAAATCTCTGCCATTCCCCATCACCAGCATGAAAGAC
ACAGAGGGGATTCCTTGCCTAGGGGACAAGGTGAAGAGTATCATAGAGGGAATTATTGAAGATGGAGAAA
GTTCTGAAGCTAAAGCTGTGTTAAATGATGAGCGATATAAATCCTTCAAACTCTTTACCTCTGTGTTTGG
TGTGGGACTGAAGACAGCTGAGAAATGGTTCAGGATGGGTTTCAGAACTCTCAGCAAAATACAATCAGAC
AAAAGCCTGAGGTTTACACAAATGCAGAAAGCAGGATTCCTCTACTACGAAGACCTCGTTAGCTGTGTGA
ACAGGCCAGAAGCCGAGGCTGTCAGCATGCTAGTTAAGGAGGCGGTTGTGACATTTCTTCCAGATGCCTT
GGTCACCATGACTGGGGGGTTCCGCAGGGGTAAGATGACTGGACATGATGTAGACTTTCTAATTACCAGC
CCAGAAGCCACAGAGGATGAAGAGCAGCAGCTCTTGCATAAAGTGACAGACTTTTGGAAGCAGCAGGGGT
TGCTTTTGTACTGCGACATCTTAGAGTCAACCTTTGAAAAGTTCAAGCAGCCCAGCAGGAAGGTGGATGC
TCTCGACCATTTCCAGAAATGCTTCCTGATTCTGAAGCTGGACCACGGGAGAGTGCACAGCGAGAAGAGC
GGCCAGCAGGAAGGAAAGGGCTGGAAGGCCATCCGTGTAGATCTGGTCATGTGCCCCTATGATCGCCGGG
CCTTCGCCCTGCTTGGATGGACCGGCTCCAGACAGTTTGAGAGAGACTTGCGGCGCTATGCCACACACGA
GAGGAAGATGATGCTGGACAACCACGCCCTGTATGACAGGACTAAGGGGAAGACTGTCACGATTTCACCT
TTGGATGGGAAAGTGTCAAAACTACAGAAAGCCTTGAGGGTGTTTCTTGAAGCAGAAAGTGAAGAAGAGA
TCTTTGCCCATCTGGGACTGGACTACATTGAACCATGGGAAAGAAATGCCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_009345
Insert Size 1593 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009345.2, NP_033371.2
RefSeq Size 2133 bp
RefSeq ORF 1593 bp
Locus ID 21673
UniProt ID P09838
Cytogenetics 19 34.32 cM
Summary Isoform TDT-S: Transferase that catalyzes the nontemplated addition of nucleoside triphosphate to coding ends during V(D)J recombination (N addition). Involved in the generation of diversity in the antigen-binding region of immunoglobulin heavy and light chains and T-cell receptors during B-and T-cell development. Does not act on double-stranded DNA with blunt ends.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_009345" in other vectors (4)
SKU Description Size Price
MG221097 Dntt (tGFP-tagged) - Mouse deoxynucleotidyltransferase terminal (Dntt) transcript variant 1, (10ug) 10 ug
$940.00
MR221097 Dntt (Myc-DDK-tagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 1 10 ug
$740.00
MR221097L3 Lenti ORF clone of Dntt (Myc-DDK-tagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 1 10 ug
$1,040.00
MR221097L4 Lenti ORF clone of Dntt (mGFP-tagged) - Mouse deoxynucleotidyltransferase, terminal (Dntt), transcript variant 1 10 ug
$1,040.00

file-alt Citations

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