Zcchc4 (NM_030185) Mouse Untagged Clone

SKU
MC217334
Zcchc4 (untagged) - Mouse zinc finger, CCHC domain containing 4 (Zcchc4), (10ug)
  $764.00
4 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Zcchc4
Synonyms 4930449I23Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217334 representing NM_030185
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCCCATGGACTGTCTTGAGTCCCTGGAGGGTGATGGCGACGCGGGGCGTAGGGCGTCCGGGG
TAGAGGTGGCGCTTCCCTCGAACCCCACGGCTCCTGCCCCGCTGTGCCCGCACGGGCCTACTCTTCTCTT
TGTAAAAGTGAACCAAGGGAAGGAAGAAACACGAAAGTTTTATGCCTGCTCAGCCTGTAGAGATAGAAAA
GACTGTAACTTTTTTCAGTGGGAAGATGAAAAGTTGTCAGAAGCTAGACTTGCTGCCCGAGAAATTCATA
ATCAAAAATGCCAGCCTCCTCTATCCCGGGCACAATGCATAGAAAGGTACCTGAGTTTCATTCAGTTGCC
CTTGGCTCAAAGAAAGTTCTGTCAAAGTTGCCAGCAGTTGCTGTTACCTGCAGACTGGAGGGAGCACGGC
ACGCATCAGCTGTCAGCTGACATTTCTGTCGCCCAGCTTGGAAGGCCCAGTCAGCTGCTCTACCCACTGG
AGAACAAGAAGACGCACGCCCAGTACCTGTTTGCTGACAGGAGCTGCCAGTTTCTGGCAGGCCTCCTTGC
TACCCTCGGATTCAGCAGAGTGTTGTGTGTTGGAGCACCAAGGCTCCACGAGCAGATCAGGCTAACAGCA
TCAGGTGAGAGGTCCGGCATGAGGAGCCTTTTATTGGACATTGACTTTCGATATTCACAGTTTTACCTGG
AAGGAAGCTTTTGCCGCTATAACATGTTCAACCATCATTTCTTTGATGGAAAGGCTGCCCTTGAAGTGTG
CAAGGAATTCTTACAGGAAGAGGAAGGCAAGGGGGTGATTATGGTGACAGACCCTCCCTTTGGCGGCTTG
GTGGAACCTCTGGCTATTACATTCAAGAAGTTAATTGCTATGTGGAAAGAAGGTCAAAGCCAAGATGACA
GCCACAAAGAACTGCCCATTTTCTGGATTTTCCCCTATTTTTTTGAATCACGGATTTGTCAGTTTTTCCC
GAGCTTCTGCATGCTGGATTACCAGGTGGATTATGATAATCATGCGCTTTACAAACATGGAAAGACAGGT
CGAAAACAGTCTCCTGTCCGAATTTTCACCAACGTTCCACCCAACAAAATAATCCTTCCATCAGAGGAAG
GATACAGATTTTGCTCCCTCTGTCAGAGATATGTCTCTCGTGAGAACCAGCACTGTGTGCATTGTAATTC
TTGCACATCCAAGGACGGCAGGAAATGGAGCCATTGCTTTCTCTGCAAAAAGTGTGTGAAGCCTTCCTGG
ATCCATTGTAACACCTGCAATCGCTGTGCACTCCCAGACCATTCCTGTTTGGGGCCTAAAGATGGCTGCT
TTATTTGTGGTGCGCTGGATCACAAACGCAGCAATTGTCCCAACATCGGCACCTCTTGGAGAGCTAACAA
GTTAGTTGAATTCTTTGGCTTTCTGGAAAAAAGATTGCTCATGTCAAGCTTCCTAGGAGCAATCTACAGA
AGTACAAAGCTTTCAGAGGACTCAGAATTTTTCAGAGTGCTAAATCGTTCCTCTCACCATTACGTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_030185
Insert Size 1539 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030185.3, NP_084461.1
RefSeq Size 5756 bp
RefSeq ORF 1539 bp
Locus ID 78796
UniProt ID Q8BKW4
Cytogenetics 5 C1
Summary rRNA N6-methyltransferase that specifically methylates the adenine in position 4220 of 28S rRNA. N6-methylation of adenine(4220) in 28S rRNA is required for translation.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_030185" in other vectors (4)
SKU Description Size Price
MG220031 Zcchc4 (tGFP-tagged) - Mouse zinc finger CCHC domain containing 4 (Zcchc4), (10ug) 10 ug
$915.00
MR220031 Zcchc4 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 4 (Zcchc4) 10 ug
$715.00
MR220031L3 Lenti ORF clone of Zcchc4 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 4 (Zcchc4) 10 ug
$1,015.00
MR220031L4 Lenti ORF clone of Zcchc4 (mGFP-tagged) - Mouse zinc finger, CCHC domain containing 4 (Zcchc4) 10 ug
$1,015.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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