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Spata2 (NM_170756) Mouse Untagged Clone

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SKU
MC217265
Spata2 (untagged) - Mouse spermatogenesis associated 2 (Spata2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $528.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Spata2
Synonyms AI504642; mKIAA0757
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217265 representing NM_170756
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATACGAAGTACAAGGATGACTTATTCCGGAAGTACGTGCAGTTCCATGAGGGCAAAGTGGACACCA
CCCCCGGCAACCAGCAGCCTGGCAGCGATGAGTACCTGCGCGTGGCAGCTGCCACCCTGCTCAGCCTGCA
CAAGGTGGACCCTTTATATCGATTTCGGCTGATCCAGTTTTATGAGGTGGTGGAGAGCTCCCTCCGCTCG
CTGAGCAGCTCCAGCCTGAGTGCTCTGCACTGCGCCTTCAGCATGCTGGAGACAATGGCCATCAACCTCT
TCCTGTTCCCCTGGAAGAAGGAGTTCCGCAGCATCAAGACCTACACTGGCCCTTTTGTTTACTATGTCAA
GTCCACGTTGCTGGAGAAGGACATCCGAGCCATTCTAAGGTTCATGGGCTACGAGCCTGAGTTGGGGACT
GTGTACAAACTCAAAGAGCTTGTGGAGTCCCTCCAGGTGAAGATGGTCTCCTTTGAGCTCTTCCTGGCCA
AGGTCGAGTGTGAGCAGATGCTGGGCATCCACTCGCAGGTGAAGGACAAGGGCTACTCAGAGCTGGACGT
GGTGGCTGAGCGCAAGGGCAGCACGGAGGATGCGCGCGGGTGCTCAGATGCTCTGCGGAGGCGGGCTGAG
AGTCGGGAGCACCTGACCACTTCCATGGCTCGTGTGGCACTCCAGAAATCAGCCAGTGAGCGAGCAGCCA
AGGACTACTACAAGCCCCGGGTGACCAAACCCTCCAGGTCTGTGGATGCCTACGACAGCTACTGGGAGAG
CAGGAAGCCCCCTTCAAAGGCCTCACTGAGTCTGCGCAAGGAGCCCCTGGCCATGGATGTAGGGGAAGAC
TTGAAGGACGAGATCATCCGCCCATCCCCCTCATTGCTGGCCATGTCCAGCTCCCCCCATGGTAGCCCTG
ATGACCTTTCCTCCATCTCCTCCATCAATGGCCTTGGCCTTCTTCGTAGTACATACTTTTCCACTCAGGA
TGACGTGGACCTGTATACGGATTCAGAACCCAGGGCCACCTACCGGAGGCAGGATGCTCTGCGGCCCGAT
GTGTGGCTGGTCAAAAATGATACCCACCCCATCTACCACAAGCGTTCACCCCCCACCAAAGAGTCTGCCC
TCTCCAAGTGCCAAAACTGCGGCCTGTCCTGCAGCTCCTCCCTCTGCCAGCGCTGTGACAGTGTGCTGGT
CTGTCCTTCGGCCTCCAAGCCCAGCGCTTTCCCCAGCAAGGCTTCTGTACACGACAGCTTGGCCCACGGG
GCACCTATGCGGGAGAAGTATGTGGGCCACCAGACTCAGGGCCTTGACCGGCTGGCACCTGTCCACTCAA
AGCCCAAGCCCTCTACCACAGCCACCTCCCGCTGTGGCTTCTGTAACCGTGCAGGTGCCACCAACACGTG
CACCCAGTGTTCAAAAGTTTCCTGTGACGCCTGCCTCGGCGCCTACCACTACGACCCGTGCTGCAGAAAG
AGTGAGCTGCACAAGTTCCTGCCCAACAGTCAGCTGAACTACAAGTCCGCCCCGTTCTCCCAGCTCGTGT
ACAGATAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_170756
Insert Size 1548 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_170756.2, NP_739562.1
RefSeq Size 3996 bp
RefSeq ORF 1548 bp
Locus ID 263876
UniProt ID Q8K004
Cytogenetics 2 H3
Summary Bridging factor that mediates the recruitment of CYLD to the LUBAC complex, thereby regulating TNF-alpha-induced necroptosis (By similarity). Acts as a direct binding intermediate that bridges RNF31/HOIP, the catalytic subunit of the LUBAC complex, and the deubiquitinase (CYLD), thereby recruiting CYLD to the TNF-R1 signaling complex (TNF-RSC) (By similarity). Required to activate the 'Met-1'- (linear) and 'Lys-63'-linked deubiquitinase activities of CYLD (PubMed:28701375). Controls the kinase activity of RIPK1 and TNF-alpha-induced necroptosis by promoting 'Met-1'-linked deubiquitination of RIPK1 by CYLD (PubMed:28701375).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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"NM_170756" in other vectors (3)
SKU Description Size Price
MR208260 Spata2 (Myc-DDK-tagged) - Mouse spermatogenesis associated 2 (Spata2) 10 ug
$289.00 $480.00
MR208260L3 Lenti ORF clone of Spata2 (Myc-DDK-tagged) - Mouse spermatogenesis associated 2 (Spata2) 10 ug
$780.00
MR208260L4 Lenti ORF clone of Spata2 (mGFP-tagged) - Mouse spermatogenesis associated 2 (Spata2) 10 ug
$780.00

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