Ogfod1 (NM_001093757) Mouse Untagged Clone

SKU
MC217076
Ogfod1 (untagged) - Mouse 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (Ogfod1), transcript variant 2, (10ug)
  $515.00
4 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ogfod1
Synonyms 4930415J21Rik; AA387199; AA939912; AW061076; mKIAA1612
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217076 representing NM_001093757
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGGGAAGCGGCCGGCGGATCCTGGCCCAGCGCGGCCAATGAAGAAGGGAAAGAAGCAGGTATCGG
CAGAGTTTTCGGACGCTGTCACTGAAGAAATCCTGAGGAAGCAGGTGGCTGAGGCCTGGAGCTGCCGGAC
ACCGTTCAGCCATGAAGCTATTGCCTTGGACATGGATCCTTTCCTTCACTGTGTGATCCCAAACTTCATC
CAAAGCCAAGACTTCTTGGAAGGACTTCACAAAGAGCTTTTGAGCCTGGACTTCCATGAAAAGTACAATG
ATTTATATAAGTTCCAACAGTCTGATGATTTGAAGAACCGAAAAGAGCCCCATATCTCAGCTTTAAGGAA
ACTTATGTTTGAAGATTTTCGGGCCTGGCTTTCTAAGGTTTCCGGCATTGACCTAGAACCAACCATTGAC
ATGTCGTGTGCGAAATACGAGTTCACTGATGCTCTGCTCTGCCATGATGATGAACTGGAGGGGCGCCGGA
TCGCTTTCATTCTGTACCTGGTTCCTTCTTGGGACAGGGACTTGGGGGGCACCCTGGACCTTTATGACAC
TGATGAACACTTGCAGCCAAAGCAGATTGTCAAGTCTCTTATCCCTTCCTGGAACAAACTGGTTTTCTTT
GAAGTGTCTCCAGTATCCTTTCACCAGCATGAAATTTTATATGAGTGGATCAACCCTGCTTACCTGGAAA
TGGATTATCAAATGCAAATTCAAGAAGAATTTGAAGAAAGGTCTGAAATTCTCCTGAAGGAGTTTCTTAA
GCCTGAGAAATTTGCAGAGGTCTGTGAAGCCTTGGAGAAAGGAGATGTGGAATGGAAAAGCCATGGTCCC
CCAAACAAAAGGTTTTATGAGAAAGCAGAAGAAAATAACCTCCCTGACGTGCTGAAGGAATGCATGGGCC
TGTTCCGCTCTGAGGCGCTCTTCCTGCTGCTCTCCAACCTCACTGGCCTGAAGCTTCACTTCTTGGCCCC
TTCAGAAGATGATGAGACTGAGGAGAAAGGAGAGGGGGAGACAGCCAGTGCTGCTGCTGGCACTGAAGAA
GGGACTAGCCGACGCCCCTCGGGGCCAGAGAATAATCAGGTGGCCGCTGGCAGTCACAGTCAAGAGAACG
GTGAACAGGCAGACCCAGAAGCACAGGAAGAGGAAGCAAAGAAAGAATCGAGCGTTCCCATGTGCCAGGG
GGAGCTGAGGCGTTGGAAGACTGGCCATTACACATTAGTCCACGACAACACCAAGACTGAATTTGCCCTG
GACCTGTTTCTGTACTGTGGCTGTGAAGGCTGGGAGCCCGAATATGGCGGCTTTACCTCCTACATTGCTA
AAGGTGAAGATGAAGAGCTGCTCATAGTGAATCCAGAAAACAACTCTTTGGCGTTGGTCTACAGAGACAG
AGAAACGCTGAGGTTTGTGAAACACATCAACCACCGCAGCCTAGAGCAGAGCAAAGCCTTCCCAAGCAGA
AGCGGCTTCTGGGACTTCGCATTCATCTACTATGAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001093757
Insert Size 1509 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001093757.1, NP_001087226.1
RefSeq Size 5200 bp
RefSeq ORF 1509 bp
Locus ID 270086
UniProt ID Q3U0K8
Cytogenetics 8 C5
Summary Prolyl 3-hydroxylase that catalyzes 3-hydroxylation of 'Pro-62' of small ribosomal subunit uS12 (RPS23), thereby regulating protein translation termination efficiency. Involved in stress granule formation.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.
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Documents
Resources
"NM_001093757" in other vectors (3)
SKU Description Size Price
MR207816 Ogfod1 (Myc-DDK-tagged) - Mouse 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (Ogfod1), transcript variant 2 10 ug
$289.00 $457.00
MR207816L3 Lenti ORF clone of Ogfod1 (Myc-DDK-tagged) - Mouse 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (Ogfod1), transcript variant 2 10 ug
$757.00
MR207816L4 Lenti ORF clone of Ogfod1 (mGFP-tagged) - Mouse 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (Ogfod1), transcript variant 2 10 ug
$757.00

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