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Krt16 (NM_008470) Mouse Untagged Clone

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SKU
MC216264
Krt16 (untagged) - Mouse keratin 16 (Krt16), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
5 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Krt16
Synonyms AI324768; CK-16; K16; Krt1-1; Krt1-16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC216264 representing NM_008470
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACCTGCAGCCGCCAGTTCACCTCCTCCAGCTCCATGAAGGGCTCCTGTGGCATCGGTGGTGGCT
CTAGCCGCATGTCCTCCATCCTGGCTGGAGGATCCTGCCGGGCTCCCAGCACCTGCGGGGGCATGTCAGT
TACCTCCTCTCGCTTCTCCTCTGGGGGAGTCTGTGGGATTGGAGGTGGCTATGGTGGGAGCTTCAGCAGC
AGCAGTTTTGGTGGAGGACTTGGTAGTGGATTTGGTGGTCGATTTGATGGATTTGGTGGTGGTTTTGGTG
CTGGTCTTGGTGGTGGTCTTGGCGGTGGTATTGGTGATGGGCTCCTGGTGGGCAGTGAGAAAGTGACCAT
GCAGAACCTCAATGACCGCCTGGCCACCTACCTGGACAAGGTGCGTGCCCTGGAAGAGGCCAACAGAGAC
CTGGAGGTGAAGATCCGGGACTGGTACCAGAGGCAGCGGCCCACTGAGATCAAAGACTACAGCCCCTACT
TCAAGACCATTGAGGACCTGAAGAGCAAGATCATTATTGCCACCCAGGAGAATGCACAGTTCACTTTGCA
GATTGACAATGCCAGGCTGGCAGCTGATGACTTCAGGACCAAGTACGAGAATGAGCTGTTCTTGCGGCAG
TCCGTGGAGGGTGACATCAATGGCCTGCGCAAGGTGCTAGATGAGCTGACCCTGTCCAGAGCTGACCTGG
AAATGCAGATTGAAAACCTCAGAGAAGAGCTGGCCTTCCTGAAGAAGAACCATGAGGAGGAGATGCTTGC
CTTGAGGGGTCAGACTGGTGGGGACGTCAATGTGGAGATGGACGCAGCCCCCGGTGTGGACCTCAGCCGC
ATTCTGAATGAGATGAGGGACCAGTATGAGCAGATGGCAGAGAAGAACCGCAGAGATGTGGAGGCCTGGT
TCCTGAGAAAGACTGAGGAGCTGAACAAAGAGGTGGCCTCTAACAGTGATCTAATCCAGAGCAACCGCAG
CGAGGTGGCTGAGCTCCGCAGGGTGTTCCAGGGCCTGGAGATTGAACTGCAGTCCCAGCTCAGCATGAAA
GCATCCTTGGAGAACAGCCTAGAAGAGACCAAAGGCAGATACTGTATGCAGCTGTCCCAGATCCAGGGTT
TGATCAGCAGTGTGGAGGAGCAGCTGGCTCAGCTTCGCTGCGAGATGGAGCAGCAGAGCCAGGAGTACAA
CATCTTGTTGGATGTGAAGACAAGGCTGGAGCAGGAGATCGCCACCTACCGCCGTCTGCTGGATGGCGAG
AATATCCACTCCTCCTCACAGCACTCCTCTGGACAGTCCTATTCTTCTCGAGAAGTCTTCTCCTCATCCT
CCCGCCAGCCCCGGTCCATCCTCAAGGAGCAAGGTTCAACCAGCTTCAGCCAGAGCCAAAGTCAGAGTTC
CAGGGACTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_008470
Insert Size 1410 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008470.1, NP_032496.1
RefSeq Size 1580 bp
RefSeq ORF 1410 bp
Locus ID 16666
UniProt ID Q9Z2K1
Cytogenetics 11 D
Summary The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The encoded protein is a cytokeratin and acts as an innate immune system effector, promoting the inflammatory response upon breach of the skin barrier. Defects in this gene are a cause of pachyonychia congenita. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2015
Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1.
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Documents
Resources
"NM_008470" in other vectors (4)
SKU Description Size Price
MG220206 Krt16 (tGFP-tagged) - Mouse keratin 16 (Krt16), (10ug) 10 ug
$657.00
MR220206 Krt16 (Myc-DDK-tagged) - Mouse keratin 16 (Krt16) 10 ug
$289.00 $457.00
MR220206L3 Lenti ORF clone of Krt16 (Myc-DDK-tagged) - Mouse keratin 16 (Krt16) 10 ug
$757.00
MR220206L4 Lenti ORF clone of Krt16 (mGFP-tagged) - Mouse keratin 16 (Krt16) 10 ug
$757.00

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