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Mief2 (NM_001009927) Mouse Untagged Clone

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SKU
MC216198
Smcr7 (untagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 7 homolog (human) (Smcr7), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Mief2
Synonyms AI482195; Gm11; Smcr7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC216198 representing NM_001009927
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGTTCTCCCAGAAGCAGAGGAAACAGAGCGGCAGCGAGGGCCTGGGCAGTGTGGTGGACTTCC
TCTTGGCTAATGCTCGATTGGTGCTAGGTGTGGGCGGGGCTGCAGTGCTGGGCATTGCTACCCTGGCTGT
AAAGCGGCTCATCGACAGGGCCACCAGCCCTCCAGATGAGGATGACACCAAGGGGGACAGCTGGAAGGAA
CTGAGCCTGCTGAGAGCCACATCGCCCCAAAAGCCCCAACCCCCTCCTGCTGCCTTCAGCCAGCCCCTAG
CTACTGGGTCCCCCTCCCCCTCTGTGCCAGTGGAGCCCACGCCCATTCATTCTCCCACGACACCTAAGTT
CAGCACTATAGCACCCCTGTGCCTGACGTTCCAGGAGAGGCTGCTGGCCTTCGAGCGCAAGCATGTGATT
ACCCCAGAAGCCCACGTGACGTTGGCGAAACAGCTGGCTGGAGACATTGCCTTGGAGCTGCAGGCCTACT
TGAGGAGCAAGTTCCCAGAACTGCCCTTTGGTGCACTCGTGCCGGGTGGGCCTCTCTACGACGGGCTCCA
GGCAGGGACTGCTGAGCACGTGCGCCTGCTGGCGCCTCTGGAGTTGGAGCCGGGGCTATGGAGCCTGGTA
CCTGGCGTGGACACTGTGGCCAGGGAGCCTCGATGCTGGGCTGTACGCAGGACTCAGCTTGAGTTTCATC
CCCGTGGGTGCAGCCCATGGGACCGCTTCCTGGTGGGGGGCTACCTCTCCTCCCGAGTCTTGCTGGAGCT
GCTACGGAAGGCCCTGTCAGCCTCTGTCAACTGGCCAGCCATTGGCAGCCTGCTCGGGTGTCTGATCTGG
CCCGATGTGGCTTCGGAGGAGCTGCTGCTCAAAGTGCAACACGAGTGCCTGGAGTTCACTTTAGCTGTGC
TCATGGTAGTCCCTGGGGCCAGCACTGATGACCGCCTGCTGCTGGCTTGGCCCCTAGAGGGGCTGGCCAG
CAATCTCTGGCTGCAGGATCTATATCCAGTAGAGACCGCCCGTTTGCGGGCCCTGGATGACCAGGATGCT
GGTACTCGGCGGAGGTTGTTGCTGCTGCTGTGTGGTATCTGTCGTGGCCATCCGGCTCTAGTGCGGCTGG
GCTGGAGCCACCTGACTCAGGTGGTTCTGCATCTGGGTGAAGAGGAAGTGGCCTGGACTGAGGAGGCCTT
GGGGGAACGGTTTCTGCAAGCCCTGGAGTTTCTGGTGGGCAGCTTGGAGCAGGCTAGCCTGCCCTGTCAC
TTCAACCCCAGTGTGAACCTCTTGGGTAACTTCCGGGAAGAGGAGATTGATGACATTGGCTACGTGCTGT
ACAGCGGCCTCCAGGTTCCTGAAAGCCTATTCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001009927
Insert Size 1365 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001009927.2, NP_001009927.1
RefSeq Size 2564 bp
RefSeq ORF 1365 bp
Locus ID 237781
UniProt ID Q5NCS9
Cytogenetics 11 B2
Summary Mitochondrial outer membrane protein which regulates mitochondrial organization. It is required for mitochondrial fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001009927" in other vectors (4)
SKU Description Size Price
MG207258 Smcr7 (tGFP-tagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 7 homolog (human) (Smcr7) 10 ug
$657.00
MR207258 Smcr7 (Myc-DDK-tagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 7 homolog (human) (Smcr7) 10 ug
$289.00 $457.00
MR207258L3 Lenti ORF clone of Smcr7 (Myc-DDK-tagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 7 homolog (human) (Smcr7) 10 ug
$757.00
MR207258L4 Lenti ORF clone of Smcr7 (mGFP-tagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 7 homolog (human) (Smcr7) 10 ug
$757.00

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