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Pramel1 (NM_031377) Mouse Untagged Clone

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SKU
MC216177
Pramel1 (untagged) - Mouse preferentially expressed antigen in melanoma-like 1 (Pramel1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pramel1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC216177 representing NM_031377
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTGCAAGACTCCACCCACACTCCAGGAACTGGCAGAGAACAGCCTCCTGAAGAACCAGGACTTGG
CTATCTCTGCTCTGGATGACATACCCTCACTTTTCTTCCCATCACTGTTCAAAAAGGCCTGCAGAAATAG
ATATGTTGGGATCATAAAGGCGATGGTGCAGGCGTGGCCCTTCCCCTGTCTTCCTCTGGGGGCCATGATC
AGTAGGAAGACTGCCTACAGGAGAATCTTAGAGATTATCCTGTATGGGCTTGATGCCTTGCTTTCCCAGA
AAGTTCCCCACAGCAGGTGCAAGCTGCAAGTGCTGGATTTACGGGTTATGCCTTTGAAGCTGTGGAACAG
GTTGCCTGTGTTTGGGACTGCTGGCTGCAGTGAGAATCCAGCAGTGGTGGGCCATTCGGGAACAGAGGTG
AAACAGCCAGTGAAGGTGCTGGTAGACTTGGTCCTCAAGGAAAGCCCACTAGATTCCACAGAGTCCTTCC
TCGTTCAGTGGGTGGATAACAGGAATGGTTTGGTGAGTTTGTGCTGTTGCAAGCTGCAGATCTGGGCTAT
GTCCATGTATTACCACAGAAAACTTTTGGAGATTTTGGATCTGGACTCTGTCCAGGAGCTGCGTATGTAC
TGCATCAGTAATCCTGTCTGCCTGCTTAACTTCGCCCCTTACTTGGGTCGCATGAGGAACCTGCGCTGCC
TCATCCTCTCTCACCTCTGGCAGACCTTCTCGATGACCCCGGTGGAGAAGCAGCAGGTTATTACCCAGTT
CACGTCTCAGTTCCTCAAACTGAAATGCCTCCAGATCCTGCATCTGGATACTGTCTTCTTCCTAGAGGGT
CATCTGGATGAGCTATTCTGGTGGCTGAAGACACCCTTAGAGACCCTGTCTGTGATTGATTGTAATCTCT
CAAAATCAGACTGGTTCCATATATCTGAGTTCCAGTGCACAAGTCAGCTAAAACACCTGAATTTGAAATG
GGTCAAACTGACCCATTTGAGCCCAGAGCCCCTTCGAGTTCTGTTACTAAAATCTGCATCTACCCTAACA
TCCCTGGATTTGGAGGGCTGTCAAATGATGGACTCTCAACTCAGTGCCATCCTACCTGCTCTGAGATGCT
GTACACAGCTCACCAAGTTTAATTTCCATGGGAACTATATCTCCATGCCTATCCTGAGGGAGCTGGCATA
TAACGTTGTCAAGCAGAAATCCCAACAGTCAAAGATACGCTTTATCCCAAGCTGTAGTCATCACAGTGGT
CTGGAGTTTGAGGCCATTTCTCAGCCTCATATTGTGTTTGTAGATGTGGATCGGACTACTGGGGAGCAAG
AACAAGTCTTGTTTTATGCTATTTGTTCTGGAGAATATGTGTTATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_031377
Insert Size 1377 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031377.2, NP_113554.1
RefSeq Size 2440 bp
RefSeq ORF 1377 bp
Locus ID 83491
UniProt ID Q99MW3
Cytogenetics 4 E1
Summary May play a role in acrosome development and also in sperm maturation and motility.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_031377" in other vectors (4)
SKU Description Size Price
MG213492 Pramel1 (tGFP-tagged) - Mouse preferentially expressed antigen in melanoma-like 1 (Pramel1), (10ug) 10 ug
$657.00
MR213492 Pramel1 (Myc-DDK-tagged) - Mouse preferentially expressed antigen in melanoma-like 1 (Pramel1) 10 ug
$289.00 $457.00
MR213492L3 Lenti ORF clone of Pramel1 (Myc-DDK-tagged) - Mouse preferentially expressed antigen in melanoma-like 1 (Pramel1) 10 ug
$757.00
MR213492L4 Lenti ORF clone of Pramel1 (mGFP-tagged) - Mouse preferentially expressed antigen in melanoma-like 1 (Pramel1) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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