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Spag4 (NM_139151) Mouse Untagged Clone

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SKU
MC215884
Spag4 (untagged) - Mouse sperm associated antigen 4 (Spag4), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Spag4
Synonyms 1700041K21Rik; mKIAA4118; MNCb-0953; Sun4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC215884 representing NM_139151
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCGGAGCCCCCGCTCAGGCTCGGCCGCATCCTCTCATAATCACACACCCAACTTCTACAGCGAGA
ACAGCAATAGTTCCCACAGCGCGACTTCGGGGGACAGCAATGGGCGCCGGTCCGCTGGGCCGGAGCTCGG
GGAGCCCGAGGGCAGAAGGGCCCGGGGCTCGAGCTGTGGTGAGCCCGCCTTAAGCCCAGGAATGCCCGGA
GGAGACACATGGGCAGGAAGCTCTCGGCCGAAGCTGGCGCCTCGGAGCCACAATGGGCAGACCGCCTGTG
GCGCGGCAACCGTGAGGGGCGGGGCCTCGGAACCGTCTGGATCTTCTGTAGTTTTGGAGGAACAACTCAA
CCTTCTCCCGATCCTGGATCTGAGGCAGGAGATGCCTACCCCGAGGGTGTCCAAGAGCTTCCTGAGCCTC
CTCTTTCAGGTGCTGAGCATGGTGTTATCCCTGGCAGTCGATGGGCTGGTCTGTGTGTGCAGGGAAATCT
GCTCCATCCGCTTCCTGTTCACTGCTGTGTCGCTCCTGAGCATCTTTCTGGCAGCACTCTGGTGGGGGCT
CCTGTACCTGATCCCTCCTTTGGAGAACGAACCTACGGAGATGCTGACTCTAAGCCAATACCACCACCGC
GTGCATTCCCAGGGCCAGCAACTGCAGCAGCTCCAGGCAGAACTGAATAAACTCCACAAGGAGGTGTCCA
GCGTTCGTGCAGCCCACAGTGAGAGAGTGGCCAAGCTCGTGTTCCAGAGGCTGAATGAAGACTTCGTGCG
CAAACCTGACTATGCACTGAGCTCTGTGGGAGCCTCCATCGACCTGGAGAAGACGTCCAGTGACTACGAG
GACCAGAACACTGCCTACTTCTGGAACCGCTTGAGCTTCTGGAACTATGCCCGCCCGCCTTCTGTCATAT
TGGAGCCAGATGTGTTCCCTGGAAATTGCTGGGCTTTTGAAGGTGATAAAGGCCAGGTGGTGATCCGACT
GCCAGGTCATGTGCAGTTAAGCGACATCACCCTGCAGCATCCTCCACCCACTGTGGCACACACCGGAGGG
GCCAGCAGCGCGCCTCGGGACTTTGCAGTCTATGGGCTCCAAGCTGATGACGAGACTGAAGTGTTCTTGG
GGAAATTCATCTTTGATGTGCAGAAATCTGAAATTCAGACTTTCCACCTCCAGAATGATCCTCCTTCTGC
CTTCCCCAAGGTGAAGATTCAGATTCTAAGCAACTGGGGCCATCCCCGCTTCACGTGCTTATATCGCGTC
CGTGCTCATGGTGTGCGGACCTCAGAGTGGGCAGACGATAATGCCACAGGGGTCACAGGGGGACCCCATT
AA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_139151
Insert Size 1332 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139151.4, NP_631890.3
RefSeq Size 1502 bp
RefSeq ORF 1332 bp
Locus ID 245865
UniProt ID Q9JJF2
Cytogenetics 2 H1
Summary Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_139151" in other vectors (4)
SKU Description Size Price
MG220664 Spag4 (tGFP-tagged) - Mouse sperm associated antigen 4 (Spag4), (10ug) 10 ug
$489.00 $657.00
MR220664 Spag4 (Myc-DDK-tagged) - Mouse sperm associated antigen 4 (Spag4) 10 ug
$289.00 $457.00
MR220664L3 Lenti ORF clone of Spag4 (Myc-DDK-tagged) - Mouse sperm associated antigen 4 (Spag4) 10 ug
$757.00
MR220664L4 Lenti ORF clone of Spag4 (mGFP-tagged) - Mouse sperm associated antigen 4 (Spag4) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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