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Gjc2 (NM_080454) Mouse Untagged Clone

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SKU
MC215861
Gjc2 (untagged) - Mouse gap junction protein, gamma 2 (Gjc2), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $732.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gjc2
Synonyms B230382L12Rik; Cx47; Gja12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC215861 representing NM_080454
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCAACATGAGCTGGAGCTTCCTGACGCGGCTGCTGGAGGAGATCCACAATCATTCCACCTTCGTGG
GCAAAGTTTGGCTCACTGTGCTGGTGGTCTTCCGCATTGTGCTGACAGCCGTCGGTGGTGAGTCCATCTA
TTCAGATGAGCAATCCAAGTTCACCTGCAACACGCGGCAACCGGGTTGTGACAACGTCTGCTATGACGCC
TTTGCGCCCCTGTCTCATGTGCGCTTCTGGGTCTTCCAGATAGTGGTCATCTCCACACCTTCTGTCATGT
ACCTGGGCTATGCAGTCCACCGCTTGGCGCGGGCCTCGGAACAGGAGCGCAGACGCGCTCTCCGACGTCG
CCCTGGCACCCGGCGCTTGCCCAGGGCGCAGCTGCCACCGCCGCCACCTGGCTGGCCGGACACCACCGAT
CTGGGAGAGGCGGAGCCCATATTGGCTCTAGAGGAGGATGAGGACGAGGAGCCGGGGGCGCCCGAGGGCC
CCGGAGAAGACACGGAGGAGGAGCGAGCGGAGGATGTGGCTGCCAAAGGGGGCGGAGGTGATGGCAAGAC
GGTGGTCACTCCTGGCCCGGCCGGGCAGCACGATGGGCGGCGGCGCATCCAGAGGGAGGGCCTGATGCGT
GTGTACGTGGCTCAGCTGGTGGTTAGGGCGGCCTTCGAGGTGGCCTTTCTGGTGGGCCAGTACCTACTGT
ACGGCTTCGAGGTGCCACCCTTCTTTGCCTGCAGCCGCCAGCCTTGCCCCCACGTAGTGGATTGCTTCGT
GTCGCGGCCGACCGAGAAGACGGTCTTCTTGCTGGTCATGTACGTGGTTAGCTGTCTATGCTTGTTGCTC
AACCTCTGTGAGATGGCGCACCTGGGTCTCGGCAGTGCGCAGGATGCTGTGCGCGGCCGTCGGGGAGCCT
CAGCGGCGGGGCCTGGCCCCACGCCGCGCCCACCGCCCTGCGCTTTCCCGGCCGCGGCCGCCGGCCTGGC
TTGCCCTCCAGACTACAGCCTGGTGGTGCGTGCAGCTGAGCGCGCGCGAGCGCACGACCAGAACTTGGCG
AACCTAGCGCTGCAGGCGTTGCGCGATGGGGCGGCGGTGGCGGCGGTTTCCGCGGACCGCGACAGTCCGC
CGTGCGCTGGGCTCAATGCAACCTCTCGGGGGGCACCCAGGGTGGGCGGCCTAGCTTCCGGAACCGGCAG
CGCCACGTCGGGGGGCACCGTTGGGGAGCAGAGCCGGCCGGGAGCTCAGGAACAACTGGCCACTAAGCCC
AGGGCTGGCTCTGAAAAGGGCAGTACAGGCAGCAGAGACGGCAAGGCCACCGTGTGGATCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_080454
Insert Size 1323 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080454.4, NP_536702.3
RefSeq Size 2188 bp
RefSeq ORF 1323 bp
Locus ID 118454
UniProt ID Q8BQU6
Cytogenetics 11 37.05 cM
Summary One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a role in myelination in central and peripheral nervous systems.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) uses a different segment for its 5' UTR, compared to variant 2. Variants 1 and 2 encode the same protein.
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Documents
Resources
"NM_080454" in other vectors (4)
SKU Description Size Price
MG226362 Gjc2 (tGFP-tagged) - Mouse gap junction protein gamma 2 (Gjc2) transcript variant 1, (10ug) 10 ug
$886.00
MR226362 Gjc2 (Myc-DDK-tagged) - Mouse gap junction protein, gamma 2 (Gjc2), transcript variant 1 10 ug
$686.00
MR226362L3 Lenti ORF clone of Gjc2 (Myc-DDK-tagged) - Mouse gap junction protein, gamma 2 (Gjc2), transcript variant 1 10 ug
$986.00
MR226362L4 Lenti ORF clone of Gjc2 (mGFP-tagged) - Mouse gap junction protein, gamma 2 (Gjc2), transcript variant 1 10 ug
$986.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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