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Gpr52 (NM_001146330) Mouse Untagged Clone

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SKU
MC215186
Gpr52 (untagged) - Mouse G protein-coupled receptor 52 (Gpr52), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $686.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gpr52
Synonyms EG620246
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC215186 representing NM_001146330
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGAATCCAGGTGGACTGAATGGAGGATCCTGAACATGAGCAGTAGCATTGTGAATGTGTCCGAGC
ACCACTCCTGCCCTCTTGGATTTGGTCACTACAGTGTGGAGGATGTCTGCATCTTTGAGACAGTTGTCAT
TGTCTTGCTGACATTTCTGATCATCTCTGGGAATTTAACAGTCATCTTTGTCTTTCACTGTGCTCCGCTG
TTACACCATTATACTACCAGCTACTTTATACAGACCATGGCATACGCTGACCTCCTCGTTGGAGTTACCT
GCTTGGTTCCTACTCTGTCCCTTCTTCATTACTCTACAGGTGTCCATGAGTCATTGACTTGCCAGGTGTT
TGGATATATTATTTCAGTTCTAAAAAGCGTGTCTATGGCATGTCTTGCTTGCATCAGTGTGGATCGATAT
CTTGCAATAACCAAGCCTCTATCCTACAATCAACTGGTCACTCCTTGTCGCCTGAGAATTTGCATTATTA
TGATTTGGATTTACTCCTGCCTAATTTTCTTGCCTTCCTTTTTTGGCTGGGGCAAACCTGGTTACCATGG
TGACATTTTTGAATGGTGTGCCACATCTTGGCTTACCAGTGCCTATTTTACTTGCTTTATTGTTTGTTTA
CTTTATGCTCCTGCTGCCTTGGTTGTCTGCTTCACTTACTTTCACATTTTCAAAATTTGCCGGCAGCACA
CCAAAGAGATAAATGACCGGAGGGCCAGATTCCCTAGTCACGAGGTAGAGGCCTCTAGGGAAGCAGGGCA
CAGCCCTGACCGTCGCTACGCCATGGTTTTATTTAGGATAACTAGCGTGTTTTACATGCTGTGGCTTCCA
TATATTATTTACTTTCTTCTAGAAAGTTCTCGTGTCTTGGACAATCCCACACTGTCCTTCTTAACCACCT
GGCTTGCGATAAGCAATAGTTTTTGTAACTGTGTAATATACAGCCTCTCCAACAGTGTTTTCCGCCTTGG
CCTCCGAAGGCTTTCTGAAACAATGTGCACATCTTGTGTGTGTGCGAAGGATCAGGAAGCACAAGATCCC
AAACCTAGGAGACGGGCAAATTCCTGCTCCATTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_001146330
Insert Size 1086 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001146330.1, NP_001139802.1
RefSeq Size 1086 bp
RefSeq ORF 1086 bp
Locus ID 620246
UniProt ID P0C5J4
Cytogenetics 1 H2.1
Summary G- protein coupled receptor activated by antipsychotics reserpine leading to an increase in intracellular cAMP and its internalization (By similarity). May play a role in locomotor activity through modulation of dopamine, NMDA and ADORA2A-induced locomotor activity. These behavioral changes are accompanied by modulation of the dopamine receptor signaling pathway in striatum (PubMed:24587241, PubMed:28583861). Modulates HTT level via cAMP-dependent but PKA independent mechanisms throught activation of RAB39B that translocates HTT to the endoplasmic reticulum, thus avoiding proteasome degradation (PubMed:25738228).UniProtKB/Swiss-Prot Function
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Documents
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"NM_001146330" in other vectors (4)
SKU Description Size Price
MG224508 Gpr52 (tGFP-tagged) - Mouse G protein-coupled receptor 52 (Gpr52), (10ug) 10 ug
$886.00
MR224508 Gpr52 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 52 (Gpr52) 10 ug
$289.00 $686.00
MR224508L3 Lenti ORF clone of Gpr52 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 52 (Gpr52) 10 ug
$986.00
MR224508L4 Lenti ORF clone of Gpr52 (mGFP-tagged) - Mouse G protein-coupled receptor 52 (Gpr52) 10 ug
$986.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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