Ccdc84 (NM_201372) Mouse Untagged Clone

SKU
MC214748
Ccdc84 (untagged) - Mouse coiled-coil domain containing 84 (Ccdc84), (10ug)
  $330.00
4 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ccdc84
Synonyms D630044F24Rik; Gm1114
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC214748 representing NM_201372
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCACCGCAGCGATGTCCTCTGTGTCGGCAAACTTTCTTCTGTGGTCGCGGTCACGTCTACAGCC
ACAAGCACCAGCGGCAGTTGAAGGGGGCTCTGGAGCGGCTTCTACCGCAGGTGGAGGCCGCCCGCCGGGC
CGTCCGCGCCGCCCAGGTGGAGCGCTATGTCCCCGAGCACGATCGGTGCTGTTGGTGCCCGTGCTGTGGC
TGCGAAGTGCGAAAACACCTGAGCCATGGCAACCTGACAGTGCTACACGGGGGTCTGCTGGAACATCTGG
CCAGCCCAGAGCACAAGAAAGCCACCAACAAATTCTGGTGGGAGAACAAGGCCAATGCCCAGATGAAAGA
GAAGTTTCTGATCTCCCCCCAGGATTATGCACGGTTTAAGAAGTCCATGGTGAAGGGCTTGGATTCCTAT
GAGGAAAAGGAGGACGAGGTGATTAAAGAGATGGCAGCTCAGATCCGTGAGGTAGAACAGAGTCGGCAGG
AAGTGGTCCGATCTGTCTTAGAGCCTCAGGCAGAGTCAGATCCAGAAGAGGGCTCTTCAGCACCTGAAAG
TTGGAAAGCGACCAACGGCCATGTAGCCTCCAGCTCACAGCAGGTATCACACTTGGCCTTGCAACCTGTT
GCAGAGCTTGATTGGATGGAGACAGGACAGCAACTAACATTCATTGGCCATCAGGATACACCCGGAATTG
GTAACATTCACTCAGGTGCCACACCTCCCTGGATGATCCAAGAGGAGGAGCACAGCTCTGGCAGCCTCCC
AATAGGACCCTCCTATGAGGAATTTCTCAAAGAAAAGGAAAAACAAAAACTGAAGAAACTCCCTCCAGAT
AGAGTTGGCGCCAACTTTGACCACAGTTCCAACACCAGTGCAGGCTGGTTGCCTTCTTTTGGCAGAGTCT
GGAATAATGGACGCCGCTGGCAGTCCAGGCATCAATTCAAAACCGAAGCTGCAACAAGAAGCAAGCAACC
ACGTAAAGGGAAAAGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_201372
Insert Size 999 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201372.3, NP_958760.2
RefSeq Size 1175 bp
RefSeq ORF 999 bp
Locus ID 382073
UniProt ID Q4VA36
Cytogenetics 9 A5.2
Summary Negative regulator of centrosome duplication. Constrains centriole number by modulating the degradation of the centrosome-duplication-associated protein SASS6 in an acetylation-dependent manner. SIRT1 deacetylates CENATAC in G1 phase, allowing for SASS6 accumulation on the centrosome and subsequent procentriole assembly. The CENATAC acetylation level is restored in mitosis by NAT10, promoting SASS6 proteasome degradation by facilitating SASS6 binding to APC/C E3 ubiquitin-protein ligase complex/FZR1.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the protein-coding transcript. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_201372" in other vectors (4)
SKU Description Size Price
MG204904 Ccdc84 (tGFP-tagged) - Mouse coiled-coil domain containing 84 (Ccdc84) 10 ug
$500.00
MR204904 Ccdc84 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 84 (Ccdc84) 10 ug
$289.00 $300.00
MR204904L3 Lenti ORF clone of Ccdc84 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 84 (Ccdc84) 10 ug
$600.00
MR204904L4 Lenti ORF clone of Ccdc84 (mGFP-tagged) - Mouse coiled-coil domain containing 84 (Ccdc84) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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