Hif1an (NM_176958) Mouse Untagged Clone
SKU
MC214417
Hif1an (untagged) - Mouse hypoxia-inducible factor 1, alpha subunit inhibitor (Hif1an), (10ug)
$732.00
3 Weeks*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Hif1an |
| Synonyms | 2310046M24Rik; A830014H24Rik; FIH; FIH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI | |
| ACCN | NM_176958 |
| Insert Size | 1050 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_176958.3, NP_795932.2 |
| RefSeq Size | 6190 bp |
| RefSeq ORF | 1050 bp |
| Locus ID | 319594 |
| UniProt ID | Q8BLR9 |
| Cytogenetics | 19 C3 |
| Summary | Hydroxylates HIF-1 alpha at 'Asn-799' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation (By similarity). Positively regulates ASB4 activity, promoting vascular differentiation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_176958" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG225292 | Hif1an (tGFP-tagged) - Mouse hypoxia-inducible factor 1 alpha subunit inhibitor (Hif1an), (10ug) | 10 ug |
$932.00
|
|
| MR225292 | Hif1an (Myc-DDK-tagged) - Mouse hypoxia-inducible factor 1, alpha subunit inhibitor (Hif1an) | 10 ug |
$732.00
|
|
| MR225292L3 | Lenti ORF clone of Hif1an (Myc-DDK-tagged) - Mouse hypoxia-inducible factor 1, alpha subunit inhibitor (Hif1an) | 10 ug |
$1,032.00
|
|
| MR225292L4 | Lenti ORF clone of Hif1an (mGFP-tagged) - Mouse hypoxia-inducible factor 1, alpha subunit inhibitor (Hif1an) | 10 ug |
$1,032.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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