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C5ar2 (NM_176912) Mouse Untagged Clone

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SKU
MC214410
C5ar2 (untagged) - Mouse G protein-coupled receptor 77 (Gpr77), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol C5ar2
Synonyms C5L2; E030029A11Rik; Gpr77
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC214410 representing NM_176912
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAATATGAGGGGCTGGGAGACGGGTGTCTGGGGGCCAGAATGATGAACCACACCACCAGCGAGTATT
ATGACTATGAGTATGACCATGAACACTACAGTGACCTTCCGGACGTGCCCGTAGACTGCCCAGCTGGCAC
CTGCTTCACTAGTGATGTCTACCTGATAGTCCTGCTTGTGCTGTATGCAGCCGTCTTCCTGGTGGGTGTG
CCAGGCAACACCCTGGTGGCGTGGGTGACCTGGAAAGAGTCCCGCCACAGGCTTGGGGCCTCTTGGTTCC
TGCACCTGACCATGGCCGACTTGCTTTGTTGTGTGTCTCTGCCCTTCCTGGCTGTGCCCATCGCCCAAAA
GGGCCACTGGCCATACGGGGCGGCAGGCTGCTGGCTGTTGTCCTCCATCACCATTCTGTCTATGTATGCC
AGTGTGCTGCTCCTGACCGGCCTCAGCGGAGACCTCTTCCTACTGGCTTTCAGGCCCTCCTGGAAGGGTG
CAGATCATCGAACATTTGGGGTGCGTGTGGTCCAGGCCTCCTCCTGGATGCTGGGCCTCTTGCTGACTGT
GCCTTCTGCTGTCTACCGTAGGCTGCTTCAGGAGCACTATCCTCCCCGACTTGTGTGTGGCATAGACTAT
GGGGGATCGGTCAGCGCCGAGGTTGCCATCACCACTGTTCGATTCCTCTTTGGCTTCCTGGGGCCATTGG
TGTTCATGGCCGGTTGTCACGGCATCCTCCAACGGCAGATGGCCCGCCGCCATTGGCCCCTGGGCACAGC
TGTCGTGGTGGGGTTTTTCATTTGCTGGACCCCTTATCACGTCCTGCGGGTAATCATTGCAGCGGCTCCT
CCGCACTCCTTGCTCCTCGCCCGGGTCCTGGAGGCGGAGCCTCTGTTTAATGGCCTGGCCCTGGCTCACA
GTGCTCTCAATCCCATAATGTTTTTGTATTTCGGAAGAAAACAACTCTGCAAGTCACTCCAGGCTGCATG
CCACTGGGCCCTGAGGGATCCACAGGATGAGGAAAGTGCGGTGACCAAGGTATCCATATCCACCAGCCAC
GAAATGGTGTCTGAGATGCCGGTGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_176912
Insert Size 1077 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_176912.4, NP_795886.2
RefSeq Size 3522 bp
RefSeq ORF 1077 bp
Locus ID 319430
UniProt ID Q8BW93
Cytogenetics 7 A2
Summary Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_176912" in other vectors (4)
SKU Description Size Price
MG217137 C5ar2 (tGFP-tagged) - Mouse G protein-coupled receptor 77 (Gpr77) transcript variant 1, (10ug) 10 ug
$657.00
MR217137 C5ar2 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 77 (Gpr77), transcript variant 1 10 ug
$289.00 $457.00
MR217137L3 Lenti ORF clone of C5ar2 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 77 (Gpr77), transcript variant 1 10 ug
$757.00
MR217137L4 Lenti ORF clone of C5ar2 (mGFP-tagged) - Mouse G protein-coupled receptor 77 (Gpr77), transcript variant 1 10 ug
$757.00

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