Tradd (NM_001033161) Mouse Untagged Clone

CAT#: MC211207

Tradd (untagged) - Mouse TNFRSF1A-associated via death domain (Tradd), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tradd
• Synonyms: 9130005N23Rik; AA930854
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211207 representing NM_001033161
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCGGTCAGAATGGCCACGAGGAGTGGGTGGGCAGTGCATACCTGTTTTTGGAGTCTGCGGTAG
  ACAAGGTGATCCTGTCTGAAGCCTACACGGATCCCAAGAAGAAGGTGGCAATATACAAGGCTCTGCAGAC
  TGCACTGTCAGAGAGTGGGGACAGCTCTGACGTACTGCAGATACTCAAGATCCACTGCAGCGACCCTCAG
  CTCATCGTCCAGTTGCGGTTCTGCGGGCGCGTGCTGTGCGGCCGCTTCCTCCAAGCCTACCGCGAGGGGG
  CGCTGCGCACCGCGCTGCAGAGGTGCATGGCCCCGGCGCTTGCCCAGGAAGCGCTGCGGTTGCAGCTGGA
  GTTGCGTGCAGGTGCGGAGCAGCTGGACAGTTGGCTGACTGATGAAGAGCGCTGTTTGAATTACATCTTA
  GCCCAGAAGCCCGACCGGCTCAGGGACGAGGAACTCGCGGAGCTGGAGGATGAGCTCTGCAAACTGACGT
  GTGACTGCACTGGCCAGGGTGGAGCCATACAGGTAGCTTCTGCAGGTTCGAAGTTCCCGGTTTCCTCTCC
  GACCGAGGAGAAACCACTGCCGGCCGCCTGCCAGACTTTTCTGTTCCACGGGCAGCTCGTAGTGAACCGG
  CCACTGACTCTTCAAGACCAGCAGACGTTTGCGCGCTCGGTGGGTCTCAAGTGGCGCAGGGTGGGGCGCT
  CGCTGCAGCGTAACTGTCGGGCACTGAGAGATCCTGCCCTCGACTCGCTGGCCTACGAGTATGAGCGTGA
  TGGGCTATACGAGCAGGCCTTCCAGCTGCTGCGCCGTTTCATGCAAGCCGAGGGCCGCCGTGCCACACTG
  CAGCGCCTGGTGGAGGCGCTGGAGGAGAACGAACTCACTAGTCTAGCAGAGGATCTGTTGGGCCAGGCGG
  AGCCGGATGGCGGCCTGGCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033161
• Insert Size: 933 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033161.2, NP_001028333.1
• RefSeq Size: 1385 bp
• RefSeq ORF: 933 bp
• Locus ID: 71609
• UniProt ID: Q3U0V2
• Cytogenetics: 8 53.04 cM
• Gene Summary: Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B (By similarity). The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A.[UniProtKB/Swiss-Prot Function]