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Ppie (NM_019489) Mouse Untagged Clone

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SKU
MC210012
Ppie (untagged) - Mouse peptidylprolyl isomerase E (cyclophilin E) (Ppie), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ppie
Synonyms 2010010D16Rik; Cyp33
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC210012 representing NM_019489
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACCACCAAGCGTGTGCTGTACGTGGGCGGGCTGGCAGAAGAGGTGGACGACAAGGTTCTTCATG
CTGCATTTATCCCCTTTGGAGACATCACAGATATTCAAATTCCTCTGGACTATGAAACAGAAAAACACCG
AGGGTTTGCTTTTGTTGAGTTTGAGTTGGCAGAGGATGCGGCAGCAGCTATCGACAACATGAATGAATCT
GAGCTCTTTGGACGGACGATTCGTGTCAATTTGGCCAAGCCAATGAGAATTAAAGAGGGCTCTTCCAGGC
CAGTCTGGTCAGATGATGACTGGTTGAAGAAGTTTTCTGGGAAGACTCTTGAGGAGAATAAGGAAGAAGA
AGGACCCGAGCCTCCCAAAGCTGAAGCCCAGGAGGGAGAGCCCACTGCAAAAAAAGCCCGGTCAAATCCT
CAGGTGTACATGGACATCAAGATTGGGAACAAGCCAGCAGGCCGCATTCAGATGCTCCTACGGTCTGATG
TGGTGCCCATGACCGCAGAAAACTTCCGCTGCCTGTGCACCCATGAAAAGGGCTTTGGCTTCAAGGGAAG
CAGCTTCCACCGCATCATCCCCCAGTTCATGTGTCAGGGTGGTGATTTCACAAACCACAATGGCACAGGG
GGCAAGTCCATCTATGGGAAGAAGTTTGATGATGAAAACTTCATCCTTAAACACACAGGACCAGGCCTCC
TCTCCATGGCCAATTCTGGCCCGAACACCAACGGCTCCCAGTTCTTCCTGACCTGTGATAAGACAGATTG
GCTGGACGGCAAGCATGTGGTATTTGGGGAGGTCACGGAAGGCCTGGATGTCTTGCGGCAGATCGAGGCT
CAGGGCAGCAAGGATGGGAAGCCAAAGCAGAAGGTGATGATTGCCGACTGTGGAGAGTACATGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_019489
Insert Size 906 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019489.5, NP_062362.1
RefSeq Size 1168 bp
RefSeq ORF 906 bp
Locus ID 56031
UniProt ID Q9QZH3
Cytogenetics 4 D2.2
Summary Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3' UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity; this requires proline isomerase activity.UniProtKB/Swiss-Prot Function
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"NM_019489" in other vectors (4)
SKU Description Size Price
MG216659 Ppie (tGFP-tagged) - Mouse peptidylprolyl isomerase E (cyclophilin E) (Ppie), (10ug) 10 ug
$500.00
MR216659 Ppie (Myc-DDK-tagged) - Mouse peptidylprolyl isomerase E (cyclophilin E) (Ppie) 10 ug
$289.00 $300.00
MR216659L3 Lenti ORF clone of Ppie (Myc-DDK-tagged) - Mouse peptidylprolyl isomerase E (cyclophilin E) (Ppie) 10 ug
$600.00
MR216659L4 Lenti ORF clone of Ppie (mGFP-tagged) - Mouse peptidylprolyl isomerase E (cyclophilin E) (Ppie) 10 ug
$600.00

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