Pdgfc (NM_019971) Mouse Untagged Clone

SKU
MC209989
Pdgfc (untagged) - Mouse platelet-derived growth factor, C polypeptide (Pdgfc), (10ug)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pdgfc
Synonyms 1110064L01Rik; AI647969; PDGF-C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC209989 representing NM_019971
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCCTCCTCGGCCTCCTCCTGCTGACATCTGCCCTGGCCGGCCAAAGAACGGGGACTCGGGCTGAGT
CCAACCTGAGCAGCAAGTTGCAGCTCTCCAGCGACAAGGAGCAGAACGGAGTGCAAGATCCCCGGCATGA
GAGAGTTGTCACTATATCTGGTAATGGGAGCATCCACAGCCCGAAGTTTCCTCATACATACCCAAGAAAT
ATGGTGCTGGTGTGGAGATTAGTTGCAGTAGATGAAAATGTGCGGATCCAGCTGACATTTGATGAGAGAT
TTGGGCTGGAAGATCCAGAAGACGATCTATGCAAGTATGATTTTGTAGAAGTTGAGGAGCCCAGTGATGG
AAGTGTTTTAGGACGCTGGTGTGGTTCTGGGACTGTGCCAGGAAAGCAGACGTCTAAAGGAAATCATATC
AGGATAAGATTTGTATCTGATGAGTATTTTCCATCTGAACCCGGATTCTGCATCCACTACAGTATTATCA
TGCCACAAGTCACAGAAACCACGAGTCCTTCGGTGTTGCCCCCTTCATCTTTGTCATTGGACCTGCTCAA
CAACGCTGTGACTGCCTTCAGTACCTTGGAAGAGCTGATTCGGTACCTAGAGCCAGATCGATGGCAGGTG
GACTTGGACAGCCTCTACAAGCCAACATGGCAGCTTTTGGGCAAGGCTTTCCTGTATGGGAAAAAAAGCA
AAGTGGTGAATCTGAATCTCCTAAAGGAAGAGGTAAAACTCTACAGCTGCACACCCCGGAACTTCTCAGT
GTCCATACGGGAAGAGCTAAAGAGGACAGATACCATATTCTGGCCAGGTTGTCTCCTGGTCAAGCGCTGT
GGAGGAAATTGTGCCTGTTGTCTCCATAATTGCAATGAATGTCAGTGTGTCCCACGTAAAGTTACAAAAA
AGTACCATGAGGTCCTTCAGTTGAGACCAAAAACTGGAGTCAAGGGATTGCATAAGTCACTCACTGATGT
GGCTCTGGAACACCACGAGGAATGTGACTGTGTGTGTAGAGGAAACGCAGGAGGGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_019971
Insert Size 1038 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC037696, AAH37696
RefSeq Size 3512 bp
RefSeq ORF 1038 bp
Locus ID 54635
UniProt ID Q8CI19
Cytogenetics 3 E3
Summary Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019971" in other vectors (4)
SKU Description Size Price
MG205185 Pdgfc (tGFP-tagged) - Mouse platelet-derived growth factor, C polypeptide (Pdgfc) 10 ug
$886.00
MR205185 Pdgfc (Myc-DDK-tagged) - Mouse platelet-derived growth factor, C polypeptide (Pdgfc) 10 ug
$686.00
MR205185L3 Lenti ORF clone of Pdgfc (Myc-DDK-tagged) - Mouse platelet-derived growth factor, C polypeptide (Pdgfc) 10 ug
$986.00
MR205185L4 Lenti ORF clone of Pdgfc (mGFP-tagged) - Mouse platelet-derived growth factor, C polypeptide (Pdgfc) 10 ug
$986.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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