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Il1f5 (NM_001146087) Mouse Untagged Clone

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SKU
MC209981
Il1f5 (untagged) - Mouse interleukin 1 family, member 5 (delta) (Il1f5), transcript variant 1, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $225.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Il1f5
Synonyms AI413231; Fil1delta; Il-1h3; IL-36Ra; Il1hy1; IL36RN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_001146087, the custom clone sequence may differ by one or more nucleotides


ATGGTTCTGAGTGGGGCACTATGCTTCCGAATGAAGGATTCAGCCTTGAAGGTACTGTATCTGCACAATA
ACCAGCTGCTGGCTGGAGGACTGCACGCAGAGAAGGTCATTAAAGGTGAGGAGATCAGTGTTGTCCCAAA
TCGGGCACTGGATGCCAGTCTGTCCCCTGTCATCCTGGGCGTTCAAGGAGGAAGCCAGTGCCTATCTTGT
GGGACAGAGAAAGGGCCAATTCTGAAACTTGAGCCAGTGAACATCATGGAGCTCTACCTCGGGGCCAAGG
AATCAAAGAGCTTCACCTTCTACCGGCGGGATATGGGTCTTACCTCCAGCTTCGAATCCGCTGCCTACCC
AGGCTGGTTCCTCTGCACCTCACCGGAAGCTGACCAGCCTGTCAGGCTCACTCAGATCCCTGAGGACCCC
GCCTGGGATGCTCCCATCACAGACTTCTACTTTCAGCAGTGTGACTAG
Restriction Sites SgfI-MluI |
ACCN NM_001146087
Insert Size 468 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC132600, AAI32601
RefSeq Size 719 bp
RefSeq ORF 471 bp
Locus ID 54450
UniProt ID Q9QYY1
Cytogenetics 2 16.31 cM
Summary Inhibits the activity of interleukin-36 (IL36A,IL36B and IL36G) by binding to receptor IL1RL2/IL-36R and preventing its association with the coreceptor IL1RAP for signaling. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor. Proposed to play a role in skin inflammation. May be involved in the innate immune response to fungal pathogens. May activate an anti-inflammatory signaling pathway by recruiting SIGIRR.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.
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Documents
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"NM_001146087" in other vectors (4)
SKU Description Size Price
MG219404 Il1f5 (tGFP-tagged) - Mouse interleukin 1 family member 5 (delta) (Il1f5) transcript variant 1, (10ug) 10 ug
$440.00
MR219404 Il1f5 (Myc-DDK-tagged) - Mouse interleukin 1 family, member 5 (delta) (Il1f5), transcript variant 1 10 ug
$240.00
MR219404L3 Lenti ORF clone of Il1f5 (Myc-DDK-tagged) - Mouse interleukin 1 family, member 5 (delta) (Il1f5), transcript variant 1 10 ug
$540.00
MR219404L4 Lenti ORF clone of Il1f5 (mGFP-tagged) - Mouse interleukin 1 family, member 5 (delta) (Il1f5), transcript variant 1 10 ug
$540.00

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