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Tnnt2 (NM_001130179) Mouse Untagged Clone

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SKU
MC209566
Tnnt2 (untagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 6, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $330.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tnnt2
Synonyms cTnT; Tnt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC209566 representing NM_001130179
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTGCCCTCAGCCAGTCCCTGCTGAGGCTGAACAGATACCTCAAGACCTGTGTGCAGTCCCTGTTC
AGAGAGCCGAGAGCATGTCTGACGCCGAGGAGGTGGTGGAGGAGTACGAGGAGGAACAGGAAGAAGCTGT
GGAAGAGCAAGAGGAGGCAGTGGAGGAGGAGGAGGCTGGTGGGGCTGAACCTGAGCCTGAGGGTGAGGCT
GAGACAGAGGAGGCCAACGTAGAAGAGGTTGGTCCTGATGAAGAAGCCAAAGATGCTGAAGAAGGTCCAG
TAGAGGACACCAAACCCAAGCCCAGCAGGCTCTTCATGCCCAACTTGGTGCCACCCAAGATCCCCGATGG
AGAGAGAGTGGACTTTGATGACATCCACAGGAAGCGCGTGGAGAAGGACCTGAATGAGCTACAGACTCTG
ATCGAGGCTCACTTCGAGAACAGGAAGAAGGAGGAAGAGGAGCTGATTTCCCTCAAAGACAGGATCGAAA
AGCGTCGGGCAGAGCGGGCCGAGCAGCAGCGTATTCGCAATGAGCGGGAGAAGGAAAGGCAGAACCGCCT
GGCTGAAGAGAGGGCCCGGCGTGAGGAGGAGGAGAACAGGAGGAAGGCTGAGGATGAGGCCCGGAAGAAG
AAGGCTCTGTCCAACATGATGCACTTTGGAGGGTACATCCAGAAGCAAGCTCAGACAGAGCGGAAGAGTG
GGAAGAGACAGACAGAGAGAGAGAAGAAGAAGAAGATCCTGGCAGAGAGGAGGAAGGCGCTGGCCATCGA
CCACCTGAATGAAGACCAACTGAGAGAGAAGGCCAAGGAGCTGTGGCAGAGTATTCACAACCTGGAGGCT
GAGAAGTTCGACCTGCAGGAAAAGTTCAAGCAGCAGAAATACGAAATCAACGTTCTGCGAAACCGGATCA
ATGACAACCAGAAAGTCTCCAAAACTCGTGGGAAGGCCAAAGTCACCGGGCGTTGGAAATAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001130179
Insert Size 972 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001130179.2, NP_001123651.4
RefSeq Size 1207 bp
RefSeq ORF 972 bp
Locus ID 21956
UniProt ID P50752
Cytogenetics 1 59.32 cM
Summary Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (6) has multiple differences in the coding region, compared to variant 1. The encoded isoform (h) is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_001130179" in other vectors (4)
SKU Description Size Price
MG223331 Tnnt2 (tGFP-tagged) - Mouse troponin T2 cardiac (Tnnt2) transcript variant 6, (10ug) 10 ug
$500.00
MR223331 Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 6 10 ug
$289.00 $300.00
MR223331L3 Lenti ORF clone of Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 6 10 ug
$600.00
MR223331L4 Lenti ORF clone of Tnnt2 (mGFP-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 6 10 ug
$600.00

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