Grb2 (NM_008163) Mouse Untagged Clone

SKU
MC208597
Grb2 (untagged) - Mouse growth factor receptor bound protein 2 (Grb2), (10ug)
  $330.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Grb2
Synonyms AA408164; Ash
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC208597 representing NM_008163
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGCCATCGCCAAATATGACTTCAAAGCTACTGCTGACGATGAGCTGAGCTTCAAAAGGGGGGACA
TCCTTAAGGTTTTGAATGAAGAATGTGACCAGAACTGGTATAAGGCAGAACTCAATGGGAAAGATGGCTT
CATCCCCAAGAATTACATAGAAATGAAACCACATCCGTGGTTTTTTGGCAAAATCCCCAGAGCCAAGGCA
GAAGAAATGCTCAGCAAACAGCGGCATGACGGGGCCTTCCTGATCCGAGAGAGCGAGAGCGCTCCTGGGG
ACTTCTCCCTGTCCGTCAAGTTTGGAAATGATGTGCAGCACTTCAAGGTGCTCCGCGACGGAGCCGGGAA
GTATTTCCTGTGGGTGGTGAAGTTTAATTCTTTGAATGAGCTGGTAGATTACCACAGATCAACATCCGTG
TCCAGGAACCAGCAGATATTCTTACGGGACATAGAACAGATGCCACAGCAGCCAACCTACGTCCAGGCGC
TCTTTGACTTTGACCCCCAGGAGGATGGCGAGCTGGGCTTTCGCAGAGGAGACTTCATTCATGTCATGGA
TAACTCAGATCCCAATTGGTGGAAAGGGGCCTGCCACGGGCAGACCGGCATGTTTCCCCGCAATTATGTC
ACCCCAGTGAACCGGAACGTCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_008163
Insert Size 654 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008163.4, NP_032189.1
RefSeq Size 2725 bp
RefSeq ORF 654 bp
Locus ID 14784
UniProt ID Q60631
Cytogenetics 11 80.91 cM
Summary The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Sep 2015
Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein.
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Documents
Resources
"NM_008163" in other vectors (4)
SKU Description Size Price
MG202420 Grb2 (tGFP-tagged) - Mouse growth factor receptor bound protein 2 (Grb2) 10 ug
$489.00 $500.00
MR202420 Grb2 (Myc-DDK-tagged) - Mouse growth factor receptor bound protein 2 (Grb2) 10 ug
$289.00 $300.00
MR202420L3 Lenti ORF clone of Grb2 (Myc-DDK-tagged) - Mouse growth factor receptor bound protein 2 (Grb2) 10 ug
$600.00
MR202420L4 Lenti ORF clone of Grb2 (mGFP-tagged) - Mouse growth factor receptor bound protein 2 (Grb2) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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