Qrfp (NM_183424) Mouse Untagged Clone

SKU
MC207900
Qrfp (untagged) - Mouse pyroglutamylated RFamide peptide (Qrfp), (10ug)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Qrfp
Synonyms P51; P518; QR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC207900 representing NM_183424
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGGCTTCCGGCCTTTGCTTTCCCTACTTCTCCCTCTGAGTGCCTGCTTTCCCCTGCTGGACAGAA
GGGGACCCACAGACATCGGTGACATCGGAGCCAGGATGAGCTGGGCCCAGCTGGCTGAGGGACACCCCCC
CAACTCGGTTCAAAATCCACAGCCACAGGCCCTGCTTGTGGTGGCCAAGGAGCAGCAGGCCTCCCACAGG
GAGCACACCGGCTTCCGTCTAGGGAGGCAAGACGGTAGCAGTGAGGCCGCAGGGTTCCTGCCCGCCGACT
CGGAGAAGGCCAGCGGCCCTCTGGGGACTCTGGCAGAGGAGCTGAGCAGCTACAGCCGGAGGAAGGGAGG
CTTCAGCTTCCGCTTTGGACGGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_183424
Insert Size 375 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC094274, AAH94274
RefSeq Size 889 bp
RefSeq ORF 375 bp
Locus ID 227717
UniProt ID Q8CE23
Cytogenetics 2 B
Summary This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. The encoded products are members of the RFamide family of neuropeptides, characterized by their common protein C-terminus consisting of an arginine (R) and an amidated phenylalanine (F). These products include the neuropeptides QRFP-26 (26RFa) and the N-terminally extended form, QRFP-43 (43RFa). Both of these neuropeptides bind to the pyroglutamylated RFamide peptide receptor (QRFPR) and may regulate blood pressure, reproduction and food intake. provided by RefSeq, Sep 2015
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Documents
Resources
"NM_183424" in other vectors (4)
SKU Description Size Price
MG200640 Qrfp (tGFP-tagged) - Mouse cDNA sequence BC094274 (BC094274) 10 ug
$350.00
MR200640 Qrfp (Myc-DDK-tagged) - Mouse pyroglutamylated RFamide peptide (Qrfp) 10 ug
$289.00
MR200640L3 Lenti ORF clone of Qrfp (Myc-DDK-tagged) - Mouse pyroglutamylated RFamide peptide (Qrfp) 10 ug
$450.00
MR200640L4 Lenti ORF clone of Qrfp (mGFP-tagged) - Mouse pyroglutamylated RFamide peptide (Qrfp) 10 ug
$450.00

file-alt Citations

Pyroglutamylated RF-amide peptide (QRFP) gene is regulated by metabolic endotoxemia
Jossart, Mulumba, Granata et al
Mol. Endocrinol. (2013)
Cited by 10
AI Summary: OriGene's pCMV6-Kan/Neo plasmid containing the mouse preproQRFP sequence was used to overexpress QRFP in RAW264.7 macrophage cells, enabling the study of QRFP gene regulation under metabolic endotoxemia conditions.
Snippet: g/mL puromycin. Overexpression of preproQRFP and generation of RAW-mQRFP cells The pCMV6-Kan/Neo plasmid containing the mouse prep- roQRFP sequence was purchased from Origene (Rockville, MD). RAW cells ... Cite SaveSaving
Showing 1-1 of 1 papers.
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