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Abhd4 (NM_134076) Mouse Untagged Clone

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SKU
MC207814
Abhd4 (untagged) - Mouse abhydrolase domain containing 4 (Abhd4), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Abhd4
Synonyms 1110035H23Rik; Abh4; AI429574
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC207814 representing NM_134076
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGATGATCTGGAGCAGCAGCCTCAGGGCTGGCTGAGTAGCTGGCTCCCCACTTGGCGCCCCACTT
CCATGTCTCAACTGAAGAATGTGGAAGCCAGGATCCTCCAGTGTCTCCAGAACAAGTTCCTGGCCCGTTA
TGTATCCCTCCCAAACCAGAACAAGATCTGGACGGTGACTGTGAGCCCAGAACAAAAGGATCGCACCCCT
CTGGTGATGGTACATGGCTTTGGGGGCGGTGTGGGCCTCTGGATCCTCAACATGGATTCACTGAGTGCCC
GCCGCACACTTCATACCTTTGATCTGCTTGGTTTTGGGCGAAGCTCAAGGCCAACATTCCCAAGGGACCC
AGAAGGAGCTGAAGATGAGTTTGTGGCCTCAATAGAGACATGGCGGGAGACCATGGGAATCCCCACCATG
ATCCTCCTGGGGCACAGTTTGGGAGGATTCCTGGCCACTTCTTACTCTATCAAGTACCCTGAAAGAGTTA
AACATCTTATCCTGGTGGATCCATGGGGCTTTCCCCTACGACCAACTGACCCTAGTGAGATCCGTGCACC
TCCCACCTGGGTCAAGGCTGTGGCATCTGTCCTGGGACGTTCCAATCCACTGGCTGTTCTTCGAGTGGCT
GGGCCTTGGGGGCCTGGGCTGGTGCAGAGATTCCGTCCAGACTTCAAGCGCAAGTTTGCAGACTTCTTTG
AGGATGACACCATCTCGGAATACATCTACCACTGCAATGCACAGAATCCCAGTGGGGAAACGGCATTCAA
AGCCATGATGGAGTCCTTTGGCTGGGCCCGGCGCCCCATGTTGGAGCGAATCCACTTAATTCGAAAAGAT
GTGCCCATCACCATGATCTATGGGGCCAACACCTGGATAGATACCAGCACAGGGAAGAAGGTGAAGATGC
AAAGGCCGGATTCCTACGTCCGAGACATGGAGATCGAGGGCGCATCCCACCACGTCTATGCTGACCAGCC
ACACATCTTCAATGCTGTGGTAGAAGAGATCTGCAACTCAGTTGACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_134076
Insert Size 1029 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_134076.2, NP_598837.2
RefSeq Size 2406 bp
RefSeq ORF 1068 bp
Locus ID 105501
UniProt ID Q8VD66
Cytogenetics 14 C2
Summary Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis (PubMed:16818490, PubMed:25853435). Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains (PubMed:16818490, PubMed:25853435). Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (PubMed:16818490).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_134076" in other vectors (4)
SKU Description Size Price
MG205135 Abhd4 (tGFP-tagged) - Mouse abhydrolase domain containing 4 (Abhd4) 10 ug
$489.00 $657.00
MR205135 Abhd4 (Myc-DDK-tagged) - Mouse abhydrolase domain containing 4 (Abhd4), transcript variant 1 10 ug
$289.00 $457.00
MR205135L3 Lenti ORF clone of Abhd4 (Myc-DDK-tagged) - Mouse abhydrolase domain containing 4 (Abhd4), transcript variant 1 10 ug
$757.00
MR205135L4 Lenti ORF clone of Abhd4 (mGFP-tagged) - Mouse abhydrolase domain containing 4 (Abhd4), transcript variant 1 10 ug
$757.00

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