Asah1 (NM_019734) Mouse Untagged Clone

SKU
MC207233
Asah1 (untagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1), (10ug)
  $503.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Asah1
Synonyms 2310081N20Rik; AC; Asah
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI |
ACCN NM_019734
Insert Size 1185 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019734.3, NP_062708.1
RefSeq Size 2264 bp
RefSeq ORF 1185 bp
Locus ID 11886
UniProt ID Q9WV54
Cytogenetics 8 A4
Summary This gene encodes acid ceramidase, an enzyme that plays a central role in ceramide metabolism. The encoded protein undergoes proteolytic processing to generate a heterodimeric enzyme comprised of alpha and beta subunits that catalyzes the hydrolysis of sphingolipid ceramide into sphingosine and free fatty acid. The homozygous disruption of this gene leads to embryonic lethality in mice whereas the heterozygous animals exhibit a progressive lipid storage disease phenotype. provided by RefSeq, Oct 2015
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"NM_019734" in other vectors (4)
SKU Description Size Price
MG206162 Asah1 (tGFP-tagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1) 10 ug
$489.00 $657.00
MR206162 Asah1 (Myc-DDK-tagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1) 10 ug
$289.00 $457.00
MR206162L3 Lenti ORF clone of Asah1 (Myc-DDK-tagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1) 10 ug
$757.00
MR206162L4 Lenti ORF clone of Asah1 (mGFP-tagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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