Pigh (BC086806) Mouse Untagged Clone

SKU
MC206975
Pigh (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class H (cDNA clone MGC:102258 IMAGE:30603877), (10ug)
  $330.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pigh
Synonyms 2210416H01Rik; A930028P05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC206975 representing BC086806.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGGACGAGAAGAGCTTCTCCGATATCTGCGGCGGCCGCCTAGCCCTGCGGTGCCGCTACTACTCA
CCGTACTGCCGCGAGTTCGGCCTCAGCTCGGCTCGGCTGTCGCTGTGCTCGCTCACCGCGGTCACCTGC
GCGGTGTGGCTGGCGGCCTACGGGCTCTTCACCCTGTGCGAGAATAGCATGGTCCTCTCTGCCACCATC
TTCATCACCATCTTAGGCCTGCTTGGTTACCTCCATTTTGTGAAGATTGACCAGGAGACTCTACTAATC
ATCGACTCACTTGGCATCCAGATGACTTCATCTTATGCATCGGGCAAAGAAAGTACCACCTTCATAGAG
ATGGACAAGGTCAAGGACATTATCATTAACGAGGCTATCTACATGCAGAAGGTCATTTACTACCTCTGC
ATTTTACTGAAGGAGCCGGGGAAGCCACACGAGATCTCTCGAGTAGTTCCTGTCTTCCAGAGTGCCAAG
CCCCGGTTGGACTGCTTGATTGAAGTATACAGAAGCTGCCAGGAGGTTCTGGCACACCAGAAAGCCACA
GCAACAAGCTTATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN BC086806
Insert Size 567 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC086806
RefSeq Size 2327 bp
RefSeq ORF 566 bp
Locus ID 110417
Cytogenetics 12 35.51 cM
MW 21.1 kDa
Summary Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC086806" in other vectors (4)
SKU Description Size Price
MG201770 Pigh (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class H (cDNA clone MGC:102258 IMAGE:30603877) 10 ug
$500.00
MR201770 Pigh (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class H (cDNA clone MGC:102258 IMAGE:30603877) 10 ug
$289.00 $300.00
MR201770L3 Lenti ORF clone of Pigh (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class H (cDNA clone MGC:102258 IMAGE:30603877) 10 ug
$600.00
MR201770L4 Lenti ORF clone of Pigh (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class H (cDNA clone MGC:102258 IMAGE:30603877) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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