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Tmem65 (BC049380) Mouse Untagged Clone

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SKU
MC206952
Tmem65 (untagged) - Mouse transmembrane protein 65 (cDNA clone MGC:28807 IMAGE:4503473), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $330.00
2 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tmem65
Synonyms 2610029O13Rik; 4930438D12Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC206952 representing BC049380.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGGCGCTGAACACGGCGCAGGGCGCGCGCGACTTCATCTACAGCCTGCACTCCACGGAGAGGAGC
TGCTTGCTCAAAGAGCTGCACCGCTTCGAGTCCATTGCCATCGCACAAGAAAAATTGGAAGCTCTACCA
CCCACTCCAGGACAGCTGAGATATGTATTCTTCCACAATGCGATACCTTTCGTAGGGTTTGGCTTTTTG
GATAACGCAATTATGATTGTTGCAGGAACCCAAATTGAATTGTCTATTGGAATTATCTTGGGGATTTCA
ACAATGGCAGCTGCTGCTTTGGGAAATCTTGTTTCAGATTTAGCTGGACTGGGCCTTGCAGGTTATGTT
GAGGCCTTGGCGTCCAGGCTAGGCCTCTCAATTCCTGATCTTACACCAAAGCAAGTTGACATGTGGCAA
ACACGTGTTAGCACACATTTGGGCAAGGCTGTTGGGGTGACGATCGGCTGCATTCTAGGAATGTTCCCG
TTGATTTTCTTTGGAGGAAGTGAAGAGGATGAGAAACTGGAAACAACAAATTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN BC049380
Insert Size 537 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC049380
RefSeq Size 2688 bp
RefSeq ORF 536 bp
Locus ID 74868
Cytogenetics 15 D1
MW 19.1 kDa
Summary May play an important role in cardiac development and function. May regulate cardiac conduction and the function of the gap junction protein GJA1. May contributes to the stability and proper localization of GJA1 to cardiac intercalated disk thereby regulating gap junction communication (PubMed:26403541). Regulates mitochondrial respiration and mitochondrial DNA copy number maintenance (By similarity).UniProtKB/Swiss-Prot Function
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"BC049380" in other vectors (4)
SKU Description Size Price
MG201580 Tmem65 (tGFP-tagged) - Mouse transmembrane protein 65 (cDNA clone MGC:28807 IMAGE:4503473) 10 ug
$500.00
MR201580 Tmem65 (Myc-DDK-tagged) - Mouse transmembrane protein 65 (cDNA clone MGC:28807 IMAGE:4503473) 10 ug
$289.00 $300.00
MR201580L3 Lenti ORF clone of Tmem65 (Myc-DDK-tagged) - Mouse transmembrane protein 65 (cDNA clone MGC:28807 IMAGE:4503473) 10 ug
$600.00
MR201580L4 Lenti ORF clone of Tmem65 (mGFP-tagged) - Mouse transmembrane protein 65 (cDNA clone MGC:28807 IMAGE:4503473) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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