Polr3g (BC030063) Mouse Untagged Clone

SKU
MC206910
Polr3g (untagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833), (10ug)
  $165.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Polr3g
Synonyms RPC7, RPC32
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC206910 representing BC030063.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCTGGCAATAAAGGAAGAGGGCGTGCTGCCTACACCTTTAATATCGAAGCTGTTGGATTCAGCAGA
GGGGAGAAGTTACCTGATGTTGTGTTGAAGCCTCCACCCCTGTTTCCTGATACAGACTACAAGCCGGTG
CCCCTGAAAACGGGAGAAGATGAGGATTACATGCTGGCCTTGAAGCAGGAGTTGAGGGAAACAGTGAAA
CGATTGCCGTACTTTATTGAACCACCCGAAGAAAAACAAGGTACGCATGCGCGTCAGGCCCTTCCGGAG
GCTGGCAGTTCAGTGTTCACAGAAGGAGCAGTGGATGCGGGGACCTGTGATCATGTGACTCAGCCGATC
ACCCAACTTGAAAAAACATTACTTACCAGAATTGTCAGAAAGGAAAAACATTTATATTTTATTGTATTG
TTTTTCTTCTTTTTTTTTTTGCAACTTACTCATAATACTTTGTGTCTTCCTTTTGTAATACAATATTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN BC030063
Insert Size 483 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC030063
RefSeq Size 753 bp
RefSeq ORF 482 bp
Locus ID 67486
Cytogenetics 13 C3
MW 18.2 kDa
Summary DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC030063" in other vectors (4)
SKU Description Size Price
MG201232 Polr3g (tGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833) 10 ug
$350.00
MR201232 Polr3g (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833) 10 ug
$289.00
MR201232L3 Lenti ORF clone of Polr3g (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833) 10 ug
$450.00
MR201232L4 Lenti ORF clone of Polr3g (mGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide G (cDNA clone MGC:41022 IMAGE:1446833) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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