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Alkbh3 (NM_026944) Mouse Untagged Clone

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SKU
MC205847
Alkbh3 (untagged) - Mouse alkB, alkylation repair homolog 3 (E. coli) (Alkbh3), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Alkbh3
Synonyms 1700108H04Rik; 1810020C19Rik; Abh3; mABH3
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC018196
GACGGCTGATTAGGGTCGTGTCGGGACCTCTGCTCAGGGCCCAGCCAGGTAGCCATCCCTTGGTCTGAAT GGGGAATGAAATCTTCTGACATCTCAGAACAGAACGCTTTTTCTTCACCATGGAGGACAAGAGACAGCGA GCCCGAGTGCAGGGAGGCTGGGCTACACCTACCAAGAGCCAGTCTGCTACTCAGCCAGCTTCCCCTGCTA GGAGCCGTCTTTCCCAGACCGCGGGGCCAGCCTGGAGGAGCAAGGAACAGCAGCAATGTGACCGACAATT TGTGTTCAAAGAACCTCAGCTGGTTGTCCGTGCAGCCCCAGAACCCAGAGTGATTGACAGGGAGGGTGTG TATGAAATCAGCCTGTCACCTACTGGTGTGTCTAGGGTGTGCTTATATCCTGGCTTTGTGGACTTGAAGG AAGCTGACTGGATCTTGGAGCAGCTTTGTAAGGATGTCCCCTGGAAACAGAGGATGGGCATCAGAGAGGA TGTAACTTATCCGCAACCAAGACTTACAGCATGGTATGGAGAGCTTCCTTACACTTACTCGAGAATCACT ATGGAACCAAATCCTCACTGGCTTCCTGTGCTGTGGACTCTGAAGAGCCGCATTGAAGAGAACACCAGCC ACACCTTCAACTCCTTGCTGTGTAATTTTTACCGGGATGAGAAGGACAGTGTGGACTGGCACAGCGACGA TGAACCATCCCTGGGGAGCTGCCCCGTCATTGCTTCCCTCAGTTTTGGTGCCACTCGGACTTTTGAGATG AGGAAGAAGCCCCCACCTGAAGAAAATGGAGACTATACATATGTGGAGAGAGTGAAGATACCATTGGATC ACGGGACCTTGCTAATCATGGAAGGAGCCACACAAGCCGACTGGCAGCACCGAGTGCCCAAGGAATACCA CTCCAGACAACCGAGAGTAAACTTAACCTTTCGGACCGTTTATCCAGACCCAAGAGGAGCCCCTCGGTGA CACTTCTACAGAGTTACCACTGAGGGGAGGGCTATGCACCTAACAGCCCCTCCCCATCTCCCGCAAGCAC TTCCGTGATGTGGCTGTTCAGAAAGCGTGGTGTTTGCTTCTCAGGTTCTACTCCTCCTGAATGAGAGCTG CCAGCACGTCACCTTGTCAACATGTCTACTACAGGAACACTTACTTTGAGACAAATCTCTAAATCAAGGT TATCATTAAGCAAATTAAAAATTGCTCTGGCTGTACTCACAAAAAAAAAAAAAAAAA
Restriction Sites EcoRI-NotI |
ACCN NM_026944
Insert Size 861 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC018196, AAH18196
RefSeq Size 1247 bp
RefSeq ORF 861 bp
Locus ID 69113
UniProt ID Q8K1E6
Cytogenetics 2 E1
Summary Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (By similarity). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:16174769). Has a strong preference for single-stranded DNA (PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron (By similarity).UniProtKB/Swiss-Prot Function
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"NM_026944" in other vectors (4)
SKU Description Size Price
MG203896 Alkbh3 (tGFP-tagged) - Mouse alkB, alkylation repair homolog 3 (E. coli) (Alkbh3) 10 ug
$500.00
MR203896 Alkbh3 (Myc-DDK-tagged) - Mouse alkB, alkylation repair homolog 3 (E. coli) (Alkbh3) 10 ug
$289.00 $300.00
MR203896L3 Lenti ORF clone of Alkbh3 (Myc-DDK-tagged) - Mouse alkB, alkylation repair homolog 3 (E. coli) (Alkbh3) 10 ug
$600.00
MR203896L4 Lenti ORF clone of Alkbh3 (mGFP-tagged) - Mouse alkB, alkylation repair homolog 3 (E. coli) (Alkbh3) 10 ug
$600.00

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