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Ube2f (NM_026454) Mouse Untagged Clone

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SKU
MC205770
Ube2f (untagged) - Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ube2f
Synonyms 2510010F15Rik; AI851109
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC016117
GCCGAGGCGGACCCCGTGCCGCCGCCCGCAAGGTCACGGTGCGGAGCTCGGGCCGTCCGGGAGGCCGAGG CCGAGGCCGCGGCGGGGAGGCCGCTGCCCCTGAAGGGAAGCGGGAGGTGGACGCGGCCCGCCACGGCGAC GGCGGGGAGGAGGGGCGGCCGGCCCGCCTGGCCCGGTTCGGTCCCTGACCCGGTTCCGCGGTGTGTCACC CGGGGTGTGGCCTTCTCCTCGGCCGGCTCAGTTTCCTCCGTCTCCGGTGGGCATTGGACCCACTCCTCGG CGCTGCCGGGAGGTTACAGCCTGAGAGCCTGAAACTCACACGGTCTGGAAGCAACCCGGGAAGAGTCCAG TTTGGGTGTCTGGAGACATGGCCCTTAACCCTGCTCTTGCCTGTCTTCCAGAAGAAAGGTGGCAGGAATG CTAACGCTGGCAAGCAAGTTGAAGCGGGATGATGGTCTCAAAGGATCCCGGACATCAGCCTCCACATCTG ACTCTACCCGGAGGGTTTCTGTGAGAGACAAGTTGCTTGTTAAAGAGGTTGCAGAACTTGAAGCTAATTT ACCTTGTACATGTAAAGTACATTTTCCTGATCCAAACAAGCTTCATTGCTTTCAGCTGACTGTAAGCCCA GATGAGGGTTACTACCAGGGTGGAAAATTTCAGTTTGAAACTGAAGTTCCCGATGCCTACAACATGGTGC CTCCCAAGGTGAAATGCTTGACTAAAATCTGGCACCCCAACATCACAGAAACGGGGGAAATATGTCTAAG TTTACTAAGAGAACATTCAATTGATGGCACTGGCTGGGCTCCCACTAGAACATTAAAGGATGTTGTTTGG GGATTAAACTCTTTATTTACCGATCTCTTGAATTTTGATGATCCACTGAATATTGAAGCTGCAGAACATC ATTTGCGGGACAAGGAGGATTTTCGGGACAAAGTGGATGAATACATCAAACGCTATGCCAGATGATAAGA GGAGAGATCGCAGGTCTGAGGACTTTGTCACAGTTGTCTCTGATGTGAAACAGCTCGAGGTAGCCCTCCT CCCCATCCTCATACTCCCTGTCAGCCCCTTGGGATTGTCCCAGTCCTGCGACCATGCTGTCCTGAAGAAG ACCCTCCTGACTGCCCACTATAGGTGGCGAACAACATAATACAGCAAGAAAGTGTGTCTGGGCCTCAAGA GAGTTGTCTGCTTCCCTTAACATGTTTACTGAAACTACTGTCTAGGCTGTCTGGTGGAATCCCTCTGAAG TTGTAATGAACTCACCACTGAGGATATAGCGCTTGCTTCCTTTCCCCCAACAATCGGTGTCCTGCACAAG TGGTGTAATGACATGTTCCATGTGACTGGCAGATTGGCAATAAGAAACCCGCTATAAACTGTGATTGGAT GCACACTCTCTTAGCTTCTTCCACGAATGTTGACCCTACCTAGATGTGAAGCTTCCCAGAATGCATAGTC ATTCACTGTAGATCTTACTGAAATGCGTATTTTATTTAATGTAAGTATATTTTGGAACAGATTTGTAATT TGTAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_026454
Insert Size 558 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC016117, AAH16117
RefSeq Size 1562 bp
RefSeq ORF 558 bp
Locus ID 67921
UniProt ID Q9CY34
Cytogenetics 1 D
Summary Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.UniProtKB/Swiss-Prot Function
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"NM_026454" in other vectors (4)
SKU Description Size Price
MG201724 Ube2f (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f) 10 ug
$500.00
MR201724 Ube2f (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f) 10 ug
$289.00 $300.00
MR201724L3 Lenti ORF clone of Ube2f (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f) 10 ug
$600.00
MR201724L4 Lenti ORF clone of Ube2f (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f) 10 ug
$600.00

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