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Thbd (NM_009378) Mouse Untagged Clone

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SKU
MC205512
Thbd (untagged) - Mouse thrombomodulin (Thbd), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $538.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Thbd
Synonyms AI385582; CD141; TM
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC019154
CCACGCGTCCGGCAGGGACTAGGAGCCTGGGCTAGAAACGTCTAGGTTGTGATAGAGGCTAGCTGCTGTC GCCGCAGGCATACGGAAACCCCTCTGCCTGGACTGGGTGTCTGCACCAGCGCAGGGCTAGCTTTTCTCGG AACTTGCACGGTGCACCCCTGGACAGCATGCTTGGGATTTTCTTTCTGGGTGTGCTGGCTCCAGCTAGCC TGGGGCTCTCCGCACTAGCCAAGCTGCAGCCCACAGGCAGTCAATGCGTGGAGCATGAGTGCTTCGCGCT TTTCCAGGGCCCCGCGACCTTCCTCGATGCCAGCCAGGCCTGCCAGCGCCTGCAAGGACATTTGATGACA GTGCGCTCCTCAGTGGCTGCCGATGTCATCTCCCTTCTGCTGAGCCAGAGCAGTATGGATTTAGGGCCCT GGATCGGTTTACAGCTCCCGCAGGGCTGTGACGACCCGGTGCATCTCGGGCCCCTGCGCGGCTTCCAGTG GGTTACTGGCGATAACCACACCAGTTACAGCAGGTGGGCGCGGCCCAACGACCAGACGGCTCCACTCTGC GGCCCTCTGTGCGTCACGGTCTCGACAGCAACTGAAGCTGCACCCGGCGAGCCGGCCTGGGAAGAGAAGC CATGCGAGACTGAGACCCAGGGTTTCCTCTGTGAGTTTTACTTCACAGCTTCCTGCAGGCCTCTGACGGT GAATACTCGCGATCCTGAGGCTGCCCACATCTCTAGTACCTACAACACCCCGTTCGGGGTCAGTGGTGCG GACTTTCAAACGCTGCCGGTAGGCAGTTCCGCCGCGGTGGAGCCCCTTGGCTTGGAGCTGGTGTGCAGGG CCCCGCCCGGAACTTCAGAGGGACACTGGGCTTGGGAAGCGACAGGAGCCTGGAATTGCAGCGTGGAGAA TGGTGGCTGTGAGTACTTGTGCAATAGGAGCACGAATGAACCCAGATGCCTCTGCCCCAGAGACATGGAC CTGCAGGCCGATGGACGTTCGTGTGCAAGACCTGTGGTTCAATCGTGCAACGAACTCTGCGAGCATTTTT GTGTCAGCAACGCTGAAGTGCCAGGCTCTTACTCCTGTATGTGTGAGACAGGCTACCAGTTGGCTGCAGA CGGACACCGGTGTGAGGACGTGGATGACTGTAAGCAGGGGCCCAATCCATGTCCCCAGCTCTGTGTTAAC ACCAAGGGCGGCTTCGAATGCTTCTGCTATGATGGCTATGAGTTGGTGGATGGAGAGTGCGTGGAGCTTC TGGATCCGTGTTTCGGATCTAACTGCGAGTTTCAGTGCCAGCCAGTGAGCCCCACCGACTACCGATGCAT CTGCGCTCCGGGCTTCGCACCCAAGCCGGATGAACCGCACAAGTGCGAAATGTTCTGCAATGAAACTTCG TGCCCAGCAGACTGTGACCCTAACTCTCCTACTGTTTGTGAATGCCCTGAAGGCTTCATCCTGGACGAGG GTTCCGTATGCACGGACATTGATGAGTGCAGTCAAGGTGAATGCTTCACCAGTGAATGTCGAAACTTCCC TGGCTCCTATGAGTGTATCTGCGGGCCTGACACAGCCCTTGCTGGTCAGATTAGCAAAGACTGTGACCCC ATCCCTGTTAGGGAAGACACCAAGGAAGAGGAGGGCTCTGGGGAGCCTCCAGTCAGCCCTACGCCAGGCT CTCCGACAGGTCCCCCTTCTGCAAGGCCAGTGCACTCTGGCGTGCTCATTGGCATCTCCATTGCCAGCCT GTCCCTGGTGGTGGCGCTTTTGGCGCTTCTCTGTCACCTGCGCAAGAAGCAGGGCGCTGCTCGCGCAGAG CTGGAGTACAAGTGCGCATCTTCCGCCAAGGAGGTAGTGCTGCAGCACGTCAGGACTGATCGGACGCTGC AGAAGTTCTGAGGGATTTGCTCCAGAGACCCAGGTGGCCTTTGTCTTTCCGGGCTCTGTACCTCTCCTCT CCTCTCTCTCTCTTCAGCCTCCCAGCTGTGTTCTCTGGCAACTTAAAAGCATCCTGGCTGGTATAATAAC CAGAGAAGAGCCCATCCTCTCAGGACAAGCGAGGGTAGGGAGGACTGGAAGCAGGACAGCCCAGTTTCTT CCAAGTAGATACTGGACAACTGGGCGGAGGTGGCAAATACAGCGGAGATCCCAGAGTACCCCAGTCCCTC ACCTCACCTCCTAGTGCTGCTGATCTGTAGGCTTGAAGGCAAACCTTGACCCCATGGGCTGGAGATGACC CAGATATTTATTTTTTTTAAAAGTATTTAGTATTTTCTTCCCTCCAGTTTTCTTCTGCTTGTAAGTCTCC AGCCCCCCACCGCTTTCTCAGTCCCTCCATTCCCCCCTCCTGTCATTCTCCTCCCCAAACCTGATCATAA CTTTGCCCTTACCGTTGTTTCCAAACTCTTATGTGAAACAGAAAATAACACTAAAAGCAGAAAACGTTCT TTTTCACTGGCTTTGGGTATTTAGTCAGAAATTTCAGGTAACCAAAGCAAAAGAATTTTAACAAAAGCTA AAATATTTCAGCTGAACACTAACTAGTCAATAGTGCTGGAATGTCACAGAAAATAAACTTAAGGAAGTAG GGTTTTTTTTTTGAAATCTTTGTTTTTGAAAAGGGTGAGCCTGGGTTTTATGATTGTTGCTGTTGTTTTG AATGGGAATGACAAAAGAGGTCATTATTGTTAAGATTTTTATGCAGGCTCTACAGTGTTATTAATTTTTG ACAGTGTTCAAAATGTGCAGAGGATCCTTTGTCCAACCCTTTGACATGACAATAGGACATTGCTATCTTC AGACATACTGGGCCACATTCATAGCTTTCCAAGGATGTATGTGGTCCTGCCTCAACATATCAGAGACTGA CAGATGGAAGCATCTTCCAGTAAAGCATGAGTTGTGTGCTTCGTGCCGAGCTGACTCTCAACTGTGCTTG TCCCTTGTAGTCCCGAAATACAAGCAATGTGCTGCTTAGGGAAACATGGAAACTTGGGAATGGAGTCTGG GGGTGCCTAGATGGGGCTTTCTTTTAATGAGACTCTTGAACAATATCTCGTAATTCAGAGGGATCTTCTA GCCCTGGCCACTGGCCTGTACACAAGAATTGGGACCTCGCTTGGGATCTGGCTAGAATTGCAAAATCCTA GCCCCCACCCCTGCCCCACCCCAGTGTGCCAGTTCATAAGAATCTGCATTTTGACAACATCCACAGGGAC ATTGTCCAGTCATTTCAGGACAACTGGTCTTAAGAGTTTCCAACCTTTGTAGAACATTTAAATGTCGGTT AATAATAAGTAGCAGGCCATGTTAAGGCCATTTATTATCAAGAAACTGAGGAATTTTCTCTGCATAGCTT TGCTTTCTGGATACAATAAAATGAGAAGGTACACACCTCTAGATAGTGCCACACAGAGTCCAGAAGGGTT TTGTTTTAAGTAAGCTAGGAATGAGTTCATATGTTAGTGTAAGGAACAAATGTATTATATGTGTATCTTT TGTAAAGAAAGGTTTTTCTTTACGGTTTTGTAAGCTCAGCATATTTGTACATATTTATTTATTGGAGTTT CGCTAGAACACAAGCAAAGCCTTTGCTTATGACGTCACATGTACAAAATAAATAGATGACAGTGTAAAAA AAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_009378
Insert Size 1734 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC019154, AAH19154
RefSeq Size 3650 bp
RefSeq ORF 1734 bp
Locus ID 21824
UniProt ID P15306
Cytogenetics 2 73.45 cM
Summary Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.UniProtKB/Swiss-Prot Function
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"NM_009378" in other vectors (4)
SKU Description Size Price
MG209049 Thbd (tGFP-tagged) - Mouse thrombomodulin (Thbd) 10 ug
$738.00
MR209049 Thbd (Myc-DDK-tagged) - Mouse thrombomodulin (Thbd) 10 ug
$289.00 $538.00
MR209049L3 Lenti ORF clone of Thbd (Myc-DDK-tagged) - Mouse thrombomodulin (Thbd) 10 ug
$838.00
MR209049L4 Lenti ORF clone of Thbd (mGFP-tagged) - Mouse thrombomodulin (Thbd) 10 ug
$838.00

file-alt Citations

Dual targeting of therapeutics to endothelial cells: collaborative enhancement of delivery and effect
Greineder, Brenza, Carnemolla et al
FASEB J. (2015)
Cited by 26
AI Summary: OriGene Technologies contributed to this study by providing the mouse thrombomodulin (mTM) cDNA, which was used to generate cells expressing recombinant thrombomodulin for the dual targeting experiments.
Snippet: To make REN-TM cells or cells expressing both PECAM/TM and ICAM/TM, we purchased a mouse (m)TM cDNA (containing the entire coding se- quence of mTM and a portion of the 59 and 39 untranslated regions;nt 87-3482) from OriGene Technologies (Rockville ... Cite SaveSaving
Showing 1-1 of 1 papers.
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