F2 (NM_010168) Mouse Untagged Clone

SKU MC205384

F2 (untagged) - Mouse coagulation factor II (F2), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: F2
• Synonyms: Cf-2; Cf2; F; FII; prot; thro
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC013662; AGCTGACACACTATGTCGCACGTCCGCGGCCTGGGCCTCCCTGGCTGCTTGGCTCTGGCTGCCCTGGTTA GCCTGGTACACAGCCAGCATGTGTTCCTGGCGCCTCAGCAAGCACTATCGCTGCTCCAGCGGGTCCGCAG AGCCAACAGTGGCTTCCTGGAGGAGCTCCGAAAGGGCAACCTAGAGCGTGAGTGCGTGGAAGAGCAGTGC AGCTATGAGGAGGCCTTTGAGGCCCTGGAGTCTCCCCAAGACACAGACGTGTTCTGGGCCAAGTACACAG TGTGTGATTCAGTGAGGAAACCTCGAGAGACTTTCATGGACTGTCTGGAAGGTCGCTGTGCTATGGATCT GGGTGTGAACTACCTTGGGACTGTGAATGTCACCCATACCGGTATCCAGTGCCAGCTGTGGCGGAGCCGC TACCCCCACAAGCCTGAAATCAACTCCACCACCCATCCTGGGGCAGACCTGAAGGAGAACTTCTGCCGCA ATCCAGACAGCAGTACCACGGGACCCTGGTGCTACACCACAGATCCTACTGTGCGCAGAGAGGAATGCAG TGTCCCTGTCTGTGGCCAGGAGGGCCGTACCACTGTGGTGATGACTCCTCGCTCTGGAGGTTCCAAGGAC AATCTGTCACCTCCACTGGGACAGTGCCTCACTGAGCGTGGCCGACTATACCAGGGGAATTTGGCTGTGA CAACACTTGGGTCCCCCTGCCTGCCCTGGAACAGCTTACCAGCCAAGACCCTGAGCAAGTACCAAGACTT CGACCCCGAGGTGAAACTCGTGGAAAACTTCTGCCGCAACCCAGACTGGGATGAGGAGGGCGCATGGTGC TATGTTGCGGGGCAGCCTGGCGACTTTGAATACTGCAACCTCAACTACTGCGAGGAGGCGGTGGGAGAGG AGAACTATGATGTGGACGAGTCCATCGCGGGACGCACCACCGATGCGGAGTTCCACACCTTCTTCAATGA GAAGACCTTCGGCCTTGGGGAGGCAGACTGTGGCCTGCGGCCTTTGTTCGAGAAGAAGTCGTTGAAAGAC ACAACCGAAAAGGAGCTTCTTGACTCTTACATAGACGGGCGCATCGTGGAGGGCTGGGACGCTGAGAAGG GTATCGCCCCCTGGCAGGTGATGCTTTTTCGGAAGAGTCCCCAAGAGCTGCTGTGTGGGGCCAGCCTTAT CAGTGACCGATGGGTCCTCACTGCTGCCCACTGCATTCTGTACCCACCCTGGGACAAGAACTTCACTGAG AATGACCTCCTGGTGCGCATTGGCAAGCATTCCCGAACCAGATATGAGCGGAATGTTGAAAAGATCTCCA TGCTGGAAAAGATCTACGTCCACCCCAGATATAACTGGCGGGAGAACCTAGACCGCGATATCGCTCTGCT CAAGCTAAAGAAACCTGTACCCTTCAGTGACTATATTCACCCCGTGTGTTTGCCAGACAAGCAGACAGTA ACCAGCTTGCTCCGGGCTGGTTATAAAGGGCGGGTGACAGGCTGGGGCAACCTTCGGGAGACATGGACAA CCAACATCAATGAGATACAGCCCAGCGTCCTGCAGGTGGTGAACCTGCCCATTGTAGAGCGGCCAGTGTG CAAGGCCTCCACCCGGATTCGAATTACTGACAACATGTTCTGTGCTGGCTTCAAGGTGAATGACACCAAG CGAGGAGATGCTTGTGAAGGTGACAGTGGAGGACCTTTTGTCATGAAGAGCCCCTTTAACAACCGCTGGT ATCAAATGGGTATTGTCTCATGGGGTGAAGGATGTGACCGGAAGGGGAAATACGGCTTCTACACGCATGT GTTCCGTCTGAAAAGGTGGATACAGAAAGTCATTGATCAATTTGGATAGGTGTGTGGGTGGGTAAAGGAC CTACACTATGGAATCCTCATTGCAAAATCACAGAAACCAATCAAATTACTTTTGTGGTTTGTTTCTGTTC TCAATAAAAGTGATTGTCAGTAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010168
• Insert Size: 1857 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC013662, AAH13662
• RefSeq Size: 1998 bp
• RefSeq ORF: 1857 bp
• Locus ID: 14061
• UniProt ID: P19221
• Cytogenetics: 2 50.63 cM
• Summary: This gene encodes a vitamin K-dependent glycoprotein coagulation factor that plays an important role in the process of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that undergoes enzymatic cleavage by the coagulation factor Xa to form an active serine protease that converts soluble fibrinogen to insoluble fibrin clot. Most of the mice lacking the encoded protein die at an embryonic stage due to defects in yolk sac vasculature, while the rare nenonates succumb to hemorrhage on the first postnatal day. [provided by RefSeq, Apr 2015]