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Pmpca (NM_173180) Mouse Untagged Clone

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SKU
MC205332
Pmpca (untagged) - Mouse peptidase (mitochondrial processing) alpha (Pmpca), nuclear gene encoding mitochondrial protein, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $489.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pmpca
Synonyms 1200002L24Rik; 4933435E07Rik; Alpha-MPP; P-55
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC010810
CGATGGCGACGGCAGTGTGGGCGGCTGCGCGGCTGCTTCGGGGATCTGCGGTGCTGTGCGCGCGGCCCAG GTTTGGATCTCCTGCACACAGACGGTTCAGCAGTGGTGCCACCTACCCCAACATCCCCCTCTCTTCTCCC TTGCCTGGAGTACCCAAGCCTATTTTTGCAACAGTTGATGGACAGGAAAAGTTTGAAACCAAAGTTACCA CTCTGGACAATGGGCTTCGTGTGGCATCTCAAAATAAGTTTGGACAATTCTGTACCGTAGGAATTCTTAT TAATTCAGGATCACGATATGAAGCGAAATATCTTAGTGGAATAGCTCATTTTTTGGAAAAATTGGCATTT TCGTCCACTGCTCGATTTGACAGCAAAGACGAAATCCTGCTTACGCTGGAAAAACATGGTGGTATCTGTG ACTGCCAGACCTCAAGAGACACCACCATGTATGCTGTGTCTGCTGACAGCAAAGGCTTGGACACTGTGGT GGATCTGTTGGCTGATGTGGTTCTGCACCCCCGACTGACAGATGAAGAAATTGAGATGACGAGGATGGCT GTGCAGTTTGAACTAGAGGACCTGAACATGAGACCTGACCCAGAGCCACTGCTCACCGAGATGATTCATG AAGCTGCTTTTAGGGAGAACACAGTGGGTCTGCACCGGTTCTGCCCTGTAGAGAACATAGCGAAGATCGA TCGGGAAGTGCTGCACTCCTACCTAAAGAACTACTACACCCCTGACCGCATGGTGCTTGCTGGGGTGGGC GTGGAGCATGAGCACCTAGTAGAATGTGCCAGAAAGTACCTCGTGGGAGCCGAGCCTGCCTGGGGAGCCC CTGGAACGGTGGACGTTGACAGATCAGTGGCACAGTACACTGGGGGGATCATCAAGGTGGAAAGAGACAT GTCAAATGTCAGCCTTGGCCCAACCCCAATTCCAGAGCTCACACACATCATGGTGGGACTGGAGAGCTGT TCCTTCCTGGAGGACGACTTCATCCCCTTTGCTGTGCTGAATATGATGATGGGAGGAGGGGGCTCCTTCT CAGCTGGAGGGCCTGGCAAAGGCATGTTCTCCAGGCTCTACCTCAATGTGCTCAATCGGCACCACTGGAT GTACAATGCAACCTCCTACCACCACAGCTATGAAGATACAGGCCTCCTGTGCATTCATGCCAGCGCAGAC CCAAGACAAGTTCGAGAAATGGTAGAAATCATCACAAAGGAGTTTATCTTGATGGGCAGGACGGTGGATT TGGTGGAGCTAGAGCGGGCCAAGACACAGCTCATGTCCATGCTCATGATGAACCTGGAGTCCAGGCCTGT GATTTTTGAGGATGTGGGGAGGCAGGTGTTGGCTACACATTCAAGAAAGCTGCCCCACGAGCTATGTACA CTCATTCGCAACGTGAAGCCAGAAGATATCAAGAGGGTTGCTTCCAAGATGCTCCGTGGCAAGCCTGCTG TGGCTGCCCTGGGTGATTTGACTGACCTACCCACCTATGAGCACATCCAGGCAGCGCTGTCCAGCAGAAA TGGACACCTGCCCAGATCATACCGGCTGTTCCGGTAGAGTCCCTTAGAGCTGGCTGTAGAGTGCTTGTGT GCATGTTACTGTTGGTGTAGACACTGCATAAGGCTAGCTAGCTGTGTGAACAGTGCCAACAGTGAAACTC TGGAAACTTGAATGAAAGTTACTGTTGCCACTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_173180
Insert Size 1575 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC010810, AAH10810
RefSeq Size 1759 bp
RefSeq ORF 1575 bp
Locus ID 66865
UniProt ID Q9DC61
Cytogenetics 2 A3
Summary Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which is required for maturation of the majority of mitochondrial precursor proteins (By similarity). Most MPP cleavage sites follow an arginine at position -2 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_173180" in other vectors (4)
SKU Description Size Price
MG208414 Pmpca (tGFP-tagged) - Mouse peptidase (mitochondrial processing) alpha (Pmpca) 10 ug
$689.00
MR208414 Pmpca (Myc-DDK-tagged) - Mouse peptidase (mitochondrial processing) alpha (Pmpca), nuclear gene encoding mitochondrial protein 10 ug
$289.00 $489.00
MR208414L3 Lenti ORF clone of Pmpca (Myc-DDK-tagged) - Mouse peptidase (mitochondrial processing) alpha (Pmpca), nuclear gene encoding mitochondrial protein 10 ug
$789.00
MR208414L4 Lenti ORF clone of Pmpca (mGFP-tagged) - Mouse peptidase (mitochondrial processing) alpha (Pmpca), nuclear gene encoding mitochondrial protein 10 ug
$789.00

file-alt Citations

Targeting the Mitochondrial Processing Peptidase as a Therapeutic Strategy for Acute Myeloid Leukemia
Ramakrishnan
Thesis (2014)
Snippet: To determine the on-target nature of MPP-α shRNAs on OCI-AML2 cell viability, mouse MPP-α cDNA in a pCMV6 Kanamycin/Neomycin construct (OriGene) was amplified by a twostage PCR using the following primer pairs to add attB1 and attB2 recombination sites at either end of PCR product – Forward 5’-AAAGCAGGCACCATGGCGACGGCAGTGTGGG-3’, Reverse 5’-ACAAGAAAGCTGGTGCCTACCGGAACAGCCGGTAT-3’ and Forward 5’- GGG...
Showing 1-1 of 1 papers.
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