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Sae1 (NM_019748) Mouse Untagged Clone

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SKU
MC205310
Sae1 (untagged) - Mouse SUMO1 activating enzyme subunit 1 (Sae1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sae1
Synonyms 2400010M20Rik; 2610044L12Rik; AL033372; AOS1; AW743391; D7Ertd177e; HSPC140; Sua1; Uble1a
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC068164
CGAGAGCGGGAGCGGGTGAGCTGAAAGCCGGCGCCATGGTAGAGAAGGAGGAGGCTGGCGGCGGCGGCGG CGGTGGCATCAGCGAGGAGGAGGCAGCGCAGTATGACCGACAGATCCGCCTGTGGGGACTGGAGGCCCAG AAGCGGCTTCGAGCTTCCCGGGTGCTGATTGTTGGCATGAAAGGACTTGGAGCTGAAATTGCCAAGAATC TTATCCTGGCAGGAGTCAAAGGGCTCACCATGCTGGACCACGAACAGGTATCTCCAGAAGACCCTGGAGC TCAGTTCTTGATTCAGACTGGGTCTGTGGGCCGCAATAGGGCCGAGGCCTCTTTGGAACGAGCCCAGAAT CTCAATCCCATGGTGGATGTAAAGGTGGACACTGAGGATGTAGAGAAGAAGCCAGAGTCCTTCTTCACGA AGTTTGATGCGGTGTGCCTGACTTGCTGCTCCAGAGATGTCATCATTAAAGTCGACCAGATCTGTCACAG AAACAGCATTAAGTTCTTCACAGGAGATGTCTTTGGGTACCATGGGTACACCTTTGCGAATCTTGGAGAG CATGAATTTGTAGAAGAGAAAACCAAAGTTGCCAAAGTAAGCCAAGGAGTGGAAGATGGACCTGAGGCCA AGAGAGCAAAGCTTGATTCATCAGAGACAACCATGGTCAAAAAGAAAGTGCTTTTTTGCCCTGTAAAAGA GGCGCTAGAAGTGGACTGGAGTGGAGAGAAAGCCAAGGCTGCCCTGAAGCGTACTGCCCCCGACTACTTT CTGCTGCAAGTGCTGCTGAAATTCCGCACAGATAAAGGGAGAGATCCGACTTCTGAGAGCTACAAGGAGG ATGCGGAGCTGCTGCTGCAGATACGGAATGACGTGTTTGACTCCCTGGGCATCAGTCCTGACCTGCTTCC CGATGACTTTGTCAGGTACTGCTTCTCTGAGATGGCCCCAGTATGTGCTGTGGTTGGAGGGATCTTGGCA CAGGAAATTGTGAAGGCCCTGTCTCAAAGGGACCCTCCTCACAACAACTTCTTCTTCTTCGACGGCATGA AGGGGAGTGGAATTGTGGAGTGCCTTGGTCCCCAGTGAGCTGAGGTGTGTACAAGGCCACCGAGTCACCT GCCCTCTCTCCGGATCATTTTTCTAGAGCAAGCAGGCCAGACTTCATCCTGCCTTTGTCTTCGAAGCTAT CAGGGAACTGCGGGGTCCCAAATATGATAATCACGTGGCACCTCTGGCATTGCTCCCTTGGGCTGTCACT GGGGACTGGCAGTGCCTAGTGAGAGAGAGCATCATACTTCATGATGTCAGAGTGTCTTGTCACGGTTTGG ACTTAACTTCCCTCTATAGTCAGCAGAGGGGATCCGAGGCCCAGTGCTGCCTGGAGAACTGAGAATAGAC CTCTGTTGTTGACCCTTCCTCAGGCTGGAGCCTGGGTGTTGGAAGGGAGAGACTGTCACCCAGTTGTCAT GTCTTTTGGTTGTGAGCCTATGCACCTTACCTTTAATGGTTGAACCATCTTTTCAGCCCAAACAGTTTAT TTCTAAAAAGTAGATTTAATGCTAGGGGCCATTATGCAGGCTTGTAATCCTAGATGTTGGGATGCCGGGG CAGGATTTACTGTAAATTTCAGGGCCAAGTTGGTCTACATAATGTACCAAGAACCTTTCTCAAAATATAA TAAAGGGCATATTCCAGAGTTAGGGTGGGGCACTGTTACCCAGCTCCTTATACTGGGCACCCGGGCCCCT GACAGGAGTGGGTTGGGGGGCAGTTGGTTTGGTCTTCCTTCTAGGAGTATGAGGGCAGCCTGTCTAGTTT GTAGATCCCCTTGGTTGCTGGATAAAGGATGATAATAAAGTGTCTTTCTCTTAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN NM_019748
Insert Size 1053 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC068164, AAH68164
RefSeq Size 1906 bp
RefSeq ORF 1053 bp
Locus ID 56459
UniProt ID Q9R1T2
Cytogenetics 7 8.88 cM
Summary The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode the same isoform (a).
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"NM_019748" in other vectors (4)
SKU Description Size Price
MG205319 Sae1 (tGFP-tagged) - Mouse SUMO1 activating enzyme subunit 1 (Sae1) 10 ug
$657.00
MR205319 Sae1 (Myc-DDK-tagged) - Mouse SUMO1 activating enzyme subunit 1 (Sae1) 10 ug
$289.00 $457.00
MR205319L3 Lenti ORF clone of Sae1 (Myc-DDK-tagged) - Mouse SUMO1 activating enzyme subunit 1 (Sae1) 10 ug
$757.00
MR205319L4 Lenti ORF clone of Sae1 (mGFP-tagged) - Mouse SUMO1 activating enzyme subunit 1 (Sae1) 10 ug
$757.00

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