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Dpysl2 (NM_009955) Mouse Untagged Clone

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SKU
MC205233
Dpysl2 (untagged) - Mouse dihydropyrimidinase-like 2 (Dpysl2), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $1,269.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dpysl2
Synonyms AI851130; Crmp2; DRP2; Musunc33; TOAD-64; Ulip2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC062955
CTCTCTCCCTCTTTCTTTTTTCCGCCCTAGCTGGATCTGTGTTGGAGGAGAGGAAAAGAGACAGAGGATT GCATCTGTTTTGAAATCTCCTAATAGCAAGACCAGTTAAGGGATTGTACCTTTTTCCTACAAATATAAAT ATATATATATTTTAAACCAAGTCTTTTTTTTCCGGCTATCTTTGCTTTAAAGCTGTCCTCTTGAGATTAC TTCCTCCCGCCCCCCGGAGAGATGTCTTATCAGGGGAAGAAAAATATTCCACGCATCACGAGCGATCGTC TTCTGATCAAAGGTGGCAAGATTGTGAATGATGACCAGTCCTTCTATGCAGACATATACATGGAAGATGG GTTGATCAAGCAAATAGGAGAAAACCTGATTGTACCAGGAGGGGTGAAGACCATCGAAGCCCACTCCAGA ATGGTGATTCCCGGAGGGATTGACGTGCATACTCGCTTCCAGATGCCTGACCAGGGAATGACATCCGCTG ATGACTTCTTCCAGGGAACCAAGGCGGCCCTGGCCGGGGGAACCACCATGATCATTGACCATGTTGTTCC TGAGCCCGGGACGAGCCTATTGGCTGCCTTTGATCAGTGGAGGGAGTGGGCTGACAGCAAGTCCTGCTGT GACTATTCGCTGCACGTGGACATCACTGAGTGGCACAAGGGCATCCAGGAGGAGATGGAAGCTCTGGTGA AGGACCACGGGGTAAACTCCTTCCTCGTGTACATGGCTTTCAAAGATCGATTCCAGCTGACGGATTCCCA GATCTATGAAGTGCTGAGCGTGATCCGGGATATCGGTGCCATAGCTCAAGTCCACGCAGAGAATGGTGAC ATCATTGCTGAGGAACAGCAGAGGATCCTGGATCTGGGCATCACGGGCCCCGAGGGACACGTGTTGAGCC GGCCAGAGGAGGTCGAGGCTGAAGCTGTGAACCGGTCCATCACTATTGCCAACCAGACCAACTGCCCTCT GTATGTCACCAAAGTGATGAGCAAGAGTGCGGCTGAAGTCATCGCTCAGGCACGGAAGAAGGGAACTGTG GTGTATGGTGAGCCCATCACGGCCAGCCTGGGGACTGATGGCTCTCATTACTGGAGCAAGAACTGGGCCA AGGCTGCGGCCTTTGTCACCTCCCCACCCTTGAGCCCCGACCCAACCACTCCAGACTTTCTCAACTCGTT GCTGTCCTGTGGAGACCTCCAAGTCACTGGCAGTGCCCACTGCACCTTCAACACTGCCCAGAAGGCTGTG GGGAAGGACAACTTCACCTTGATTCCCGAGGGCACCAACGGCACTGAGGAGCGGATGTCTGTCATTTGGG ATAAAGCTGTGGTCACTGGGAAGATGGATGAGAATCAGTTTGTGGCTGTGACCAGCACCAACGCAGCCAA AGTCTTCAACCTTTACCCCCGGAAAGGTCGCATCTCGGTGGGATCTGATGCTGACTTGGTCATCTGGGAC CCTGACAGTGTGAAGACCATCTCTGCCAAGACACACAACAGTGCTCTTGAGTACAACATCTTTGAAGGCA TGGAGTGTCGCGGCTCCCCACTGGTGGTCATCAGCCAGGGCAAGATTGTCCTGGAGGACGGCACACTTCA TGTCACTGAAGGCTCAGGACGCTACATTCCCCGGAAGCCCTTCCCTGACTTTGTGTACAAACGCATCAAA GCAAGGAGCAGGCTGGCTGAGCTGAGAGGGGTCCCTCGTGGCCTGTATGACGGACCGGTATGCGAGGTGT CTGTGACGCCCAAGACGGTGACTCCAGCCTCATCAGCTAAGACATCCCCTGCCAAGCAGCAGGCACCACC TGTTCGGAACCTGCACCAGTCTGGATTCAGCTTGTCTGGTGCTCAGATTGACGACAACATTCCCCGCCGC ACCACCCAGCGCATCGTGGCACCCCCTGGTGGCCGTGCCAACATCACCAGCCTGGGCTAAAGCCCCTAGG CCTGCAGGCCACTTGGGGATGGGGGATGGGACACCTGAGGACATTCTGAGACTTCCTTTCTTCCATTTTT TTTTTTTTTAAAGAGCCTGTGATAGTTGCTGTGGGCAGCCAGTTCCTGGGGCTTCCTCTTGGGCCCCCTG CACTCGGTCTCCCCTGGAGTTTCTGAATTCTCTCACCCAAGTCCCTACACAGTCATGAACACCACACCCA AGCCTAGCCATCCACCCCACACGGAGCTGCATCCAACATGCAGACATGCGCCACCAAGCAGATCCCAGCA AGGGTGCCCCAGTCACATCCTTGGCCATGCAGTTAGCACCTTCCTGTCATGGGGAAGATATAGTGAATTG CCCTGAGCTGCCTTCTTTTCTTTTTAAAAATTTTTAAATAAGGTTTTCTTTGTGGGACTGGGGAGGGGTG GGGAGGGTGGGAGAGATTTTTTTTTTTTTTAATACTAAATTGAAAGCCTGATTCAATATTAATCCTCGGG TCTTGAACTGGACATCCTAATGATGCAATTATGTAACCATTAAGCTGATTCCGAGGCTGGCAGGCTAGGG CCGCCCCTCTGGAAAGGTTCCATGTGTCTCCATCCCCATCCCTTACTCTTCGGGTCAGCTGTTGAGAAGA GGCGGGTTTTCTTCATTGGCCTAGATTTTGCGGCAGATTAGACCTTTTGAAGGTTCTCCACCATTTTCGT ACGTCTCCTGTCCTGGTTTTTTCTTCTGCACTCTTGGAGAGATTTAGATGTTGGTCTCCTGGTTTGTGTT TCTCCGAGACAATGCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTCTTAACTCTGTCTGCTC TTAAAGCCAGGCAACCCAGTTTGGTCTCAGGCATAGCCTGAGCAGATCTTAGTTTTGCCCCCACCACCAT AGTTTTGTGCTATCCTAGTTTCTGTTAAGATTGGTGCTATCCAATTCCCATCACTAGGAGGTGGCTGAGC TTGAGGCTTGCCAGACACAGGAATGGCCCCGATTATGTCACCAATATTGTCACATGTGGGCCAAGAATAG AGTCACATGTGGTGGAAAGATGAGGAGTTAGGCTGTAGAAGTGACAGTTTCCAGTCAAATCTGTAAACCC AGCCCCCAAGCTGAGACCAGTCTCCCTGTGGCGGAGCCAGCCAAGATGGGGCCATTCTCTCTGTGGAGGA GCCCCTTCCAGCCTTTGTCAGGAAGTGTCTTTGGGGATTCTAGAATGTTGGGACTTCTCAGTGTGTGTGT TGTGGTGGGGGAACTTTTTTTTTTTTTCTGTACAGTTTTTTTTTTTTTTTTTTTTCTCAAAAGGTCTAAC ATGGTGGTGGAAGGTAGAAATCTTTGGGATACTTAGACTTCAAGTCTTTTACAAGAGGGACAACCCTATT TTGGGAAACAATATCTCATGGGAAGAAAACTGTACACACTGAACGCATAGCCCGCTTAGAGATCACATCG CCTCTTAGAGACCATGTCACCTCCTATCTGTCACCCTTCCTCTTCCTGTCACAGTGACAGGTCTTCACTA GCTGGCTTCTTTCTGTACTTGCTGACTTCTGTCATATTTCTTTCCAGATGTGTTTACAGGTCCCTGTAAG CAGTGTTAGGTCCATAGGCTTTTATGACTCCAGTCTGCAAGCAGTGTTAGGTTGACCTCATAGGCTTTTA TGATTCCAGTCTGCTGGTATGAAGCAGAGCATCAAAAAAAATTCGTGATTCCTTGAGTGTCCACACGTTT TGTCTTTGGGGGTTAAGGGTTGGGATTTTGGGTTTGTTGTTAATTTTGTTGTTTTAAAGGTAAATTGCAC TTTTAAAAATTGGTTGACTTAATATATTTGCTCTTTTTTTTTTTTTACCTGCACTTAGAGAGGAAATTTG AACACCAGAAACCACTTTTTTTTTCTCCCTGTTTATTTGTTTGGATTCTGAGAAACTTGCAGCTCTTCAC CATTTACTCCTGATGTTTCTTGTATGGGGCCGTGGCCCTTTTGGTTGTTTTTGGTCATTTTCTTAAAAAC ACAACACAACACAATACAACACAACAGTTTTAAACCTGAAGATTTCCGCAGTCCGTGTAAGCATGTTTAC CCGTTGGCTCGCTTTCTGTGTCTGTTAAATGAATGTCATATGTAAATGCTAAACTAAGTCAACAGTGTCT CAGAACTGAATAACTGCAGTGACTTGATGCTCTAAATCATTGTAGGATTTACCGCTAGATGGTTTTGAAC CCTTGAACCTGTGATTGTGACCCATGAGGGGGGACCTTTCAGTGCTAAAGCTGATCATGTGTGTAGCCAG AAAGGTACTTTTTTTCTTTTAATTTTTGTTTTATTTTTTGTAACCACTGTCTTAATGGCAAAATAATTAT GGTAAAATACAAGTCTCGTGTTTATTATTCCTTAAGAAGTCTGTGTTATATTACCACGGAACACCCAATA AAGCAAAATGTGGTTGTTTCAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN NM_009955
Insert Size 4450 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC062955, AAH62955
RefSeq Size 4450 bp
RefSeq ORF 4450 bp
Locus ID 12934
UniProt ID O08553
Cytogenetics 14 34.6 cM
Summary Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.UniProtKB/Swiss-Prot Function
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Resources
"NM_009955" in other vectors (4)
SKU Description Size Price
MG209003 Dpysl2 (tGFP-tagged) - Mouse dihydropyrimidinase-like 2 (Dpysl2) 10 ug
$489.00 $732.00
MR209003 Dpysl2 (Myc-DDK-tagged) - Mouse dihydropyrimidinase-like 2 (Dpysl2) 10 ug
$289.00 $532.00
MR209003L3 Lenti ORF clone of Dpysl2 (Myc-DDK-tagged) - Mouse dihydropyrimidinase-like 2 (Dpysl2) 10 ug
$832.00
MR209003L4 Lenti ORF clone of Dpysl2 (mGFP-tagged) - Mouse dihydropyrimidinase-like 2 (Dpysl2) 10 ug
$832.00

file-alt Citations

PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells
Winkler, Shimobayashi, Kapfhammer
Mol. Neurobiol. (2020)
Cited by 16
AI Summary: OriGene’s pCMV6-Dpysl2 (NM_009955) Mouse Untagged Clone (MC205233) was used as a template to amplify the CRMP2 coding sequence, enabling the study of CRMP2’s role in dendritic outgrowth in cerebellar Purkinje cells.
Snippet: pCMV6-Dpysl2 (NM_009955) Mouse Untagged Clone (Origene, Rockville, USA;MC205233) was used as a template and the CRMP2 coding sequence was amplified using the following primers: CRMP2-ORF-pL7-F: 5′-GTC CGG ACT CAG ATC TAT GTC TTA TCA;- pCMV6-Dpysl2 (NM_009955) Mouse Untagged Clone (Origene, Rockville, USA
Showing 1-1 of 1 papers.
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