Cybb (NM_007807) Mouse Untagged Clone
$797.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Cybb |
| Synonyms | C88302; Cgd; CGD91-phox; Cyd; gp91-1; gp91 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | RsrII-NotI | |
| ACCN | NM_007807 |
| Insert Size | 1713 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC071229, AAH71229 |
| RefSeq Size | 3098 bp |
| RefSeq ORF | 1713 bp |
| Locus ID | 13058 |
| UniProt ID | Q61093 |
| Cytogenetics | X A1.1 |
| Summary | This gene encodes the heavy chain component of a heterodimeric transmembrane ion transporter composed of both a heavy and a light chain. This transporter mediates the transfer of electrons from nicotinamide adenine dinucleotide phosphate (NADPH) to oxygen to generate superoxide. This reaction is important in the innate immune response to pathogens. However, increased activity of the encoded protein also leads to the generation of reactive oxygen species that result in oxidative stress and can cause tissue damage. Conversely, loss of function of the related gene in human causes chronic granulomatous disease. Alternative splicing results in multiple transcript variants, although the full-length nature of some of these variants has not been determined. provided by RefSeq, May 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007807" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG208975 | Cybb (tGFP-tagged) - Mouse cytochrome b-245, beta polypeptide (Cybb) | 10 ug |
$996.00
|
|
| MR208975 | Cybb (Myc-DDK-tagged) - Mouse cytochrome b-245, beta polypeptide (Cybb) | 10 ug |
$796.00
|
|
| MR208975L3 | Lenti ORF clone of Cybb (Myc-DDK-tagged) - Mouse cytochrome b-245, beta polypeptide (Cybb) | 10 ug |
$1,096.00
|
|
| MR208975L4 | Lenti ORF clone of Cybb (mGFP-tagged) - Mouse cytochrome b-245, beta polypeptide (Cybb) | 10 ug |
$1,096.00
|
|
Citations
Optimal ROS Signaling Is Critical for Nuclear Reprogramming
Zhou, Meng, Li et al
Cell Rep (2016)
Zhou, Meng, Li et al
Cell Rep (2016)
Cited by 143
AI Summary: OriGene's Nox2 (Cybb) plasmids were used to overexpress Nox2 in mouse embryonic fibroblasts, enabling the authors to study the effects of increased ROS generation on nuclear reprogramming efficiency.
Snippet: To overexpress Nox2 in secondary Dox-inducible MEFs, cells were plated in a 6-well plate at 3 × 10 5 cells/well. After 12 hr serum starvation, Nox2 (Cybb, from Origene) plasmids were introduced via FuGENE 6 according to manufacturer's instruction. ... Cite SaveSaving
CIKS (Act1 or TRAF3IP2) mediates Angiotensin-II-induced Interleukin-18 expression, and Nox2-dependent cardiomyocyte hypertrophy
Valente, Clark, Siddesha et al
J. Mol. Cell. Cardiol. (2012) 53 (1), 113-24 DOI: 10.1016/j.yjmcc.2012.04.009
Valente, Clark, Siddesha et al
J. Mol. Cell. Cardiol. (2012) 53 (1), 113-24 DOI: 10.1016/j.yjmcc.2012.04.009
Cited by 56
AI Summary: OriGene contributed to this study by providing the mouse Nox2 cDNA plasmid (catalog #MC204867), which was used for in vitro assays to investigate the molecular mechanisms underlying Angiotensin-II-induced cardiomyocyte hypertrophy.
Snippet: in the Supplement. 2.10. AT1/Nox2 binding interaction in vitro—GST pull-down assay. A plasmid containing the cDNA for mouse Nox2 was obtained from Origene Technologies, Rockville, MD (#MC204867). The Nox2 coding
Showing 1-2 of 2 papers.
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