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Itpkc (NM_181593) Mouse Untagged Clone

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SKU
MC204707
Itpkc (untagged) - Mouse inositol 1,4,5-trisphosphate 3-kinase C (Itpkc), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $932.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Itpkc
Synonyms 9130023N17Rik
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC053450
GCTCGGCGGGCCGGAACCCACGAGTTGGGGCTCCCCAGCCACACCCCTCGTAGGATTTTAAAGGGGAAGG AGGGGCCGGACGGGCCAAAGCCCGGGCCCTGGGAGCGGGTATGAGGCGCTGCCCGTGCCGGGGGAGCCTG AGCGAGGCGGAGGCCGGCGCACTGCCAGCCGAGGCCCGCATGGGGCTGGAGGCGCTGCGAGGTGGGCGGC GGCGACAGCCTGGACTACAACGACCTGGACCGGGAGCAGGTGGCCCGACGGGACGGCCGGAGGGGGGCGG TCCCCGGGCCTGGATCGAGGAGTCCAGCCTCCACTCCGAGGCGGAGAGGACCGACCTCGAGCCTGCGCCG TGTCCGAACGGTCCACAGGCAGAATCCTGTGGAGATGGACACGCGGAGTGCGAAGCAGCTGGCCTAGTAG TTGCGTCGGAGAAGCCCAGGCAAAATAAGGAGCTAGACGGGTCCAACCTCCAGACACATCCAAGAAGGAA CAGCCCCCTGGTTGAGATGGAGATGGCCGGTTCCTGGACAGATGGCTTTCGAACTGATCTTCACAGATCT GACCTCCAGTCTCGGCCAAAGAGAGCCAGCCTCTGTACCCAACCAGGGTTTGATGAGTCCTGGACCGAAC TGGACAGATCCGATATGTGGCAGACTCTGCCAGAGAGGGACAACAAGCCCAGGGTTGATAACCTTAGGAC CCACCATGGCGTGTCCAAACTCCAAACTCACCCAGTCTGCCTCTCACCAGAATCAAGTGCTGATAACTCC GGCAAAGAATTGTCCGCGGATGCTTCCAGGACTCCACATGACACCGACGGCTTCTGGATAGAGTCCCAAA CTGATGACTCTCTCAAAGGACCTAGTACTCAGACAGCCTGCAGACAACCTGGGAGTGATGGTTTTTCCTC AAAAGACACTGAGAGTGCCTTGACTCAACCTGGCACTGATGGCCTCCGGGATGACAGCGTTTTGGGGGAG TCCAATGGAAATGATCCATTAGATTTATCAGAGCCTGGAGAGTTGGTGACTAACCTGTGCTCCCACCTGG AGTGCAGCTCCCTGTGTCCTGTGCCCCGCCTTATCATCACCCCAGAGACTCCTGAACCTGAGGCCCAGCC GGTGGGACCCCAATCCCGGATTGAGGGGGGCACTGGCGGCTTCTCTTCTGCCTCCTCTTTTGATGAATCT GAGGATGACTTGGTAGCCGGGGGCGGAGGTACCAGCGATCCTGAGGACAGAGCTGGAAGCAAGCCCTGGA AGAAGCTGAAGACAGTTCTGAAGTACTCGCCCTTCGTGGTCTCCTTCCATAAGCACTACTATCCCTGGGT CCAGCTTTCCGGACACGCTGGGAACTTCCAGGCCGGTGAGGATGGTCGGATTCTGAAACGTTTCTGTCAG TGTGAGCAGCGCAGCCTGGAGCTGCTGATGGGAGACCCCCTGCGGCCTTTTGTGCCTGCTTATTATGGCA TGGTGCAGCGGGATGGCCAGGCCTTCAACCAGATGGAAGATCTCCTGGCAGACTTTGAGGGCCCCTCTAT CATGGACTGCAAGATGGGCAGCAGGACCTACCTGGAAGAGGAGCTGGTGAAGGCCAGGGAACGACCCAAG CCCCGGAAGGATATGTATGAGAAGATGGTGGCAGTGGACCCCGGGGCTCCCACCCCAGAAGAGCATGCTC AGGGTGCCATCACGAAGCCCCGATACATGCAGTGGAGGGAGACCCTGAGTTCAACTTCCACGCTGGGCTT CCGGATTGAAGGCATCAAGAAGGCTGATGGGACCTGCAACACCAACTTCAAGAAGACACAGGCTCTGGAG CAGGTGACAAAGGTGTTAGAAGACTTTGTGAATGGGGACCTTGGAATCCTAAGAAAGTACGTGGCGCGAC TGGAAGACCTCCGAGAGACTCTGGAAAATTCTCCCTTCTTCAAGACCCACGAGGTAGTGGGCAGTTCCCT TCTCTTTGTGCATGACCACACTGGCCTGGCCAAGGTCTGGATGATTGACTTCGGCAAGACAGTGGCCCTG CCTGACCACCAGATGCTCAGTCACAGGCTGCCTTGGACAGAGGGCAACCGTGAGGACGGCTACCTCTGGG GTCTGGACAATTTGATCTGCCTCCTTCAGGGGCTGGCCCAGAGCTGACGTCATCTGCCACTGCTAGGCTC ACTTGCAGGATAGCATGTGTCTGGCTAGAAGAGTCCTAAGATGTTGTCTGGGGGAAGATGAGGTGCTTCA AAGTGTCACCAGATCAGTGAGGCTTGAGGGGCTTGAGGGTCCCTGGCAGCACGGGGCATCCTGACCAGCT AGGAAAGCCTCTAAGGGAGTACCAGAGGCCACAGCTGAAGAATCAACTCTTCCCTGAGTCAGCTCTTTGA GCCTAGAGCCAAAGGGTTGGCTTTTGCTTCCCAGAGGACCAGGCATCATTTGTGAAGACCTGCAGGAGCT ACTTGTCCACCTGGGCCAGTAAGATCCCCATCCTGGTGCTGAGAGGAGGATGTCTTCCTGGGACGCTTGC TGATGCTACCTGAGGCCTAGTGCTTCTGCACAGGGCCAGCTTGCCCTCCTCCCTGCCAGGGCGGGGAAGG ATCCCTTCTGTTCCTTGTGGACTCCGAGCTACTGCAGACCCAGGCCACAGCCATACCCATCTGTACCAGC ACTGTGACCATCCATCTTCCTTCCTTGGTGCACCAGCTCCAGCCTCAGGACTTCCTCCTCCTGGCCCTGA CAGCCCAGCTCTTGTCCCAGCAGAATCCAGTGACAGGAAGGAGTTTCTGAGGCAGGGGAGGAGGCTTCTC CATGGGAACCAGACAGCCTTGCTTCACTGTATAAGTGCCCTGATCACACGCAGAATGAAGTGCCAGGTTG CTCAGAAGCACAAAGGGTGTGGCTACTGGCCCTAACCATGGACTACGTGGTTCTAACCAAAGACTCTAGA ACTCTGGGGTGGGGGAGAAACAATGTGTTCTGTGCTCCAGAACCTCGGCTTCCTGGCCCATATGGATGGG CTTGGCAAGGAACCTACCTCTTCTCTAAGGTGAAGCCCTAAGGTCTGCCAAGGCTGACACGTCTTCCTTT TCTACTGACAGACTTTATATATATATATTTATATACGTGGTAGTTACCTGGGGTGGTGGTGTCAGGGAAT TGAGGCAAAATGACTTTACTGTACCTCCGGCCTACAGTGGCACCCCAATAAACAGGACAAAATCAACAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_181593
Insert Size 2037 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC053450, AAH53450
RefSeq Size 3260 bp
RefSeq ORF 2037 bp
Locus ID 233011
UniProt ID Q7TS72
Cytogenetics 7 A3
Summary Can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_181593" in other vectors (4)
SKU Description Size Price
MG209975 Itpkc (tGFP-tagged) - Mouse inositol 1,4,5-trisphosphate 3-kinase C (Itpkc) 10 ug
$1,131.00
MR209975 Itpkc (Myc-DDK-tagged) - Mouse inositol 1,4,5-trisphosphate 3-kinase C (Itpkc) 10 ug
$931.00
MR209975L3 Lenti ORF clone of Itpkc (Myc-DDK-tagged) - Mouse inositol 1,4,5-trisphosphate 3-kinase C (Itpkc) 10 ug
$1,231.00
MR209975L4 Lenti ORF clone of Itpkc (mGFP-tagged) - Mouse inositol 1,4,5-trisphosphate 3-kinase C (Itpkc) 10 ug
$1,231.00

file-alt Citations

Specific expression and function of inositol 1,4,5-trisphosphate 3-kinase C (ITPKC) in wild type and knock-out mice
Scoumanne, Molina-Ortiz, Monteyne et al
Adv Biol Regul (2016)
Cited by 12
AI Summary: OriGene’s pCMV6-mItpkc cDNA vector (BC053450) was used for transient transfection to study ITPKC expression and function in mouse cells in this paper.
Snippet: 2 mM l-glutamine (Gibco) in an incubator with 5% CO 2 at 37 °C. For transient transfection, cells were seeded at 10 6 cells per well in a 6-well plate and transfected with FuGENE 6 transfection reagent (Promega), using 1 μg pCMV6-mItpkc cDNA vector (OriGene BC053450 ... Cite SaveSaving
Showing 1-1 of 1 papers.
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