Chek2 (NM_016681) Mouse Untagged Clone

SKU MC204618

Chek2 (untagged) - Mouse CHK2 checkpoint homolog (S. pombe) (Chek2), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Chek2
• Synonyms: Cds1; CHK2; HUCDS1; Rad53
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC056617; GTCGACCCACGCGTCCGAGGCTGTGCAGTGTTTGCGGCTCCTGGTGGTCTCACGCGGTCGGATATGAAGA GTCATCATCAGTCCCACAGCAGCACCTCCTCGAAGGCTCATGACAGTGCTTCCTGTTCACAGTCCCAGGG TGGCTTCAGCCAGCCCCAAGGCACTCCTTCTCAGTTGCACGAGCTCTCACAGTATCAGGGTTCGTCCAGC TCCTCTACCGGCACAGTGCCGTCCTCCAGCCAGTCCTCGCACTCCAGTTCTGGGACTCTGAGCTCGCTGG AGACAGTGTCTACCCAGGAACTCTGTTCTATTCCTGAGGACCAAGAACCTGAAGAACCTGGTCCTGCCCC TTGGGCTCGGCTATGGGCTCTTCAGGATGGATTCTCCAATCTAGACTGTGTTAACGACAACTACTGGTTT GGGAGAGATAAAAGCTGTGAATATTGCTTCGATGGACCACTGTTGAGAAGGACGGACAAGTACCGGACTT ACAGCAAGAAGCATTTTCGTATTTTCAGGGAAATGGGCCCTAAAAATTGTTACATCGTATACATAGAGGA TCACAGTGGAAATGGAACCTTCGTAAATACCGAGCTTATTGGGAAAGGCAAACGCTGTCCTCTGAGTAAC AACTCTGAAATCGCACTTTCACTATGTAGAAATAAAGTTTTTGTATTTTTTGATCTGACTGTAGATGATC AGTCAGTTTATCCTAAGGAATTAAGAGACGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCGTGTGG GGAGGTAAAGATGGCTTTTGAGAGGAAGACATGTCAGAAAGTGGCCATAAAGATCATTAGCAAGCGGAGG TTTGCTCTTGGCTCATCGAGAGAAGCCGACACAGCTCCCAGTGTGGAAACTGAAATAGAAATTTTGAAGA AACTAAATCATCCATGCATCATCAAGATTAAAGATGTTTTTGATGCGGAAGATTATTACATTGTTCTGGA ACTGATGGAAGGAGGAGAACTATTTGACCGGGTGGTGGGGAACAAGCGCCTGAAAGAAGCCACCTGTAAG CTCTACTTCTACCAGATGCTTGTAGCTGTACAGTACCTTCACGAAAATGGGATCATACATCGGGACTTAA AGCCGGAGAATGTTCTTTTATCATCTCAGGAAGAGGATTGTCTAATCAAGATCACTGACTTTGGGCAGTC CAAGATCTTGGGGGAGACCTCCTTGATGAGAACCTTATGTGGTACGCCCACTTATCTGGCTCCTGAGGTT CTTGTCTCCAACGGGACTGCTGGGTACAGCCGCGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTCA TCTGCCTAAGTGGGTATCCACCTTTCTCTGAGCATAAGACCCAAGTGTCCCTGAAGGATCAGATCACCAG TGGAAAGTACAACTTTATTCCTGAAGTCTGGACAGATGTCTCAGAGGAGGCTCTGGACCTTGTCAAGAAA CTGTTAGTTGTAGACCCAAAGGCTCGGCTTACCACAGAGGAGGCCTTAAATCATCCGTGGCTTCAGGATG AGTACATGAAGAAGAAATTTCAGGATCTCCTGGTGCAGGAAAAGAACTCGGTGACCCTCCCTGTGGCTCC CGCCCAGACTTCCAGTCAAAAGCGGCCCCTGGAACTGGAGGTGGAGGGTATGCCGAGCACAAAACGCCTG TCTGTGTGTGGGGCTGTGTTGTGAGCTTTGAATGTGAAAGAAATGTGCTTCCTTCCACGCTCCAATTGTC TTCTCCATCTTGACATCTTTTTTTTATATGTATATATTTTTTAAAAAATATAGCCAGGCAGTTGTGGCCC ACTCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCAAGGCCAGCCTGGTCTAC AGAGTGAGTTCCAGGATAGCCAGGACTACACAGAGAAACCCTGTCTTGGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_016681
• Insert Size: 1641 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC056617, AAH56617
• RefSeq Size: 1954 bp
• RefSeq ORF: 1641 bp
• Locus ID: 50883
• UniProt ID: Q9Z265
• Cytogenetics: 5 F
• Summary: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (By similarity).[UniProtKB/Swiss-Prot Function]