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Chek2 (NM_016681) Mouse Untagged Clone

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SKU
MC204618
Chek2 (untagged) - Mouse CHK2 checkpoint homolog (S. pombe) (Chek2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $509.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Chek2
Synonyms Cds1; CHK2; HUCDS1; Rad53
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC056617
GTCGACCCACGCGTCCGAGGCTGTGCAGTGTTTGCGGCTCCTGGTGGTCTCACGCGGTCGGATATGAAGA GTCATCATCAGTCCCACAGCAGCACCTCCTCGAAGGCTCATGACAGTGCTTCCTGTTCACAGTCCCAGGG TGGCTTCAGCCAGCCCCAAGGCACTCCTTCTCAGTTGCACGAGCTCTCACAGTATCAGGGTTCGTCCAGC TCCTCTACCGGCACAGTGCCGTCCTCCAGCCAGTCCTCGCACTCCAGTTCTGGGACTCTGAGCTCGCTGG AGACAGTGTCTACCCAGGAACTCTGTTCTATTCCTGAGGACCAAGAACCTGAAGAACCTGGTCCTGCCCC TTGGGCTCGGCTATGGGCTCTTCAGGATGGATTCTCCAATCTAGACTGTGTTAACGACAACTACTGGTTT GGGAGAGATAAAAGCTGTGAATATTGCTTCGATGGACCACTGTTGAGAAGGACGGACAAGTACCGGACTT ACAGCAAGAAGCATTTTCGTATTTTCAGGGAAATGGGCCCTAAAAATTGTTACATCGTATACATAGAGGA TCACAGTGGAAATGGAACCTTCGTAAATACCGAGCTTATTGGGAAAGGCAAACGCTGTCCTCTGAGTAAC AACTCTGAAATCGCACTTTCACTATGTAGAAATAAAGTTTTTGTATTTTTTGATCTGACTGTAGATGATC AGTCAGTTTATCCTAAGGAATTAAGAGACGAATACATCATGTCAAAAACTCTTGGAAGTGGTGCGTGTGG GGAGGTAAAGATGGCTTTTGAGAGGAAGACATGTCAGAAAGTGGCCATAAAGATCATTAGCAAGCGGAGG TTTGCTCTTGGCTCATCGAGAGAAGCCGACACAGCTCCCAGTGTGGAAACTGAAATAGAAATTTTGAAGA AACTAAATCATCCATGCATCATCAAGATTAAAGATGTTTTTGATGCGGAAGATTATTACATTGTTCTGGA ACTGATGGAAGGAGGAGAACTATTTGACCGGGTGGTGGGGAACAAGCGCCTGAAAGAAGCCACCTGTAAG CTCTACTTCTACCAGATGCTTGTAGCTGTACAGTACCTTCACGAAAATGGGATCATACATCGGGACTTAA AGCCGGAGAATGTTCTTTTATCATCTCAGGAAGAGGATTGTCTAATCAAGATCACTGACTTTGGGCAGTC CAAGATCTTGGGGGAGACCTCCTTGATGAGAACCTTATGTGGTACGCCCACTTATCTGGCTCCTGAGGTT CTTGTCTCCAACGGGACTGCTGGGTACAGCCGCGCTGTGGACTGCTGGAGTTTAGGAGTTATTCTTTTCA TCTGCCTAAGTGGGTATCCACCTTTCTCTGAGCATAAGACCCAAGTGTCCCTGAAGGATCAGATCACCAG TGGAAAGTACAACTTTATTCCTGAAGTCTGGACAGATGTCTCAGAGGAGGCTCTGGACCTTGTCAAGAAA CTGTTAGTTGTAGACCCAAAGGCTCGGCTTACCACAGAGGAGGCCTTAAATCATCCGTGGCTTCAGGATG AGTACATGAAGAAGAAATTTCAGGATCTCCTGGTGCAGGAAAAGAACTCGGTGACCCTCCCTGTGGCTCC CGCCCAGACTTCCAGTCAAAAGCGGCCCCTGGAACTGGAGGTGGAGGGTATGCCGAGCACAAAACGCCTG TCTGTGTGTGGGGCTGTGTTGTGAGCTTTGAATGTGAAAGAAATGTGCTTCCTTCCACGCTCCAATTGTC TTCTCCATCTTGACATCTTTTTTTTATATGTATATATTTTTTAAAAAATATAGCCAGGCAGTTGTGGCCC ACTCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCAAGGCCAGCCTGGTCTAC AGAGTGAGTTCCAGGATAGCCAGGACTACACAGAGAAACCCTGTCTTGGAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_016681
Insert Size 1641 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC056617, AAH56617
RefSeq Size 1954 bp
RefSeq ORF 1641 bp
Locus ID 50883
UniProt ID Q9Z265
Cytogenetics 5 F
Summary Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence L-X-R-X-X-S/T. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (By similarity).UniProtKB/Swiss-Prot Function
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Resources
"NM_016681" in other vectors (4)
SKU Description Size Price
MG208692 Chek2 (tGFP-tagged) - Mouse CHK2 checkpoint homolog (S. pombe) (Chek2) 10 ug
$708.00
MR208692 Chek2 (Myc-DDK-tagged) - Mouse CHK2 checkpoint homolog (S. pombe) (Chek2) 10 ug
$289.00 $508.00
MR208692L3 Lenti ORF clone of Chek2 (Myc-DDK-tagged) - Mouse CHK2 checkpoint homolog (S. pombe) (Chek2) 10 ug
$808.00
MR208692L4 Lenti ORF clone of Chek2 (mGFP-tagged) - Mouse CHK2 checkpoint homolog (S. pombe) (Chek2) 10 ug
$808.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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