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Cep85 (NM_144527) Mouse Untagged Clone

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SKU
MC204579
Cep85 (untagged) - Mouse coiled-coil domain containing 21 (Ccdc21), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $696.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Cep85
Synonyms 2410030J07Rik; AI173272; AI173504; Ccdc21
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC031729
CCCAGAGTGAGTGGGGTCGCCGCTCGCTCTCAGGCCTGTTTGTCTCAGGTAACTGATGGCCATGCAAGAA AAATACCCAAATGACAGGTCTCATGCTACTTCACCAGGTTCCAATGTGATTCAAAAAGGCAGTTCTCTGG GAACTGAGTGGCAGACACCAGTTATCTCAGAGACCTTTCGGAGCCGCTTCAGCCGCTGCTCAAGCATTGC TGACAGTGGGGACACAGCCATTGGGACATCATGCTCAGACATTGCAGAGGATTTTTGCAGCTCAAGTGGC AGCCCTTCCTTCCAGCCAATCAAAAGCCACATAACCATTCCAACAGCCCATGTGATGCCTTCTACTTTGG GGGCCTCTCCTGCCAAACCAAATTCTGCACCTTCCGGGCCTTCTTCCGCTAAGCTGCCCTTGTCAGGATT GACTGAAGGCGTGGGGATGACAAGAAATGGAGACTTCGGAGCTGTGAAGCGCTCTCCAGGCCTAGCAAGG GATTTTATGTACCTCCCTAGTGCTGCCGGGGAGAATGGGAGTCAGCAGTCCTGGTTCCCAGCAGTGGGCC ACGAGAGAGAGGGGGAGATGAGGAAGTTCGATGTTCCTAGCATGGAGTCTACCCTTAACCAGCCAGCCAT GCTAGAGACGTTATACTCAGATCCACATTACCGAGCCCATTTCCCCAACCCAAGACCTGATACAAATAAG GATGTATACAAAGTATTGCCAGAATCCAAGAAGGCACCGGGCAGTGGTGCAGTATTTGAGAGGAACGGAC CACATGCTAGCAGTAGTGGGGTGCTCCCTTTGGGACTCCAGCCTGCGCCTGGACTTTCCAAGTCACTATC CTCTCAGGTGTGGCAACCAAGTCCTGACCCTTGGCATCCTGGAGAACAATCCTGTGAACTCAGTACTTGT CGACAGCAGTTGGAATTGATCCGTTTACAGATGGAGCAAATGCAGAACGGAGCCATGTGTCACCATCCTG CTGCTTTCGCTCCATTACTGCCCACCCTAGAGCCAGCACAGTGGCTCAGCATCCTGAACAGTAACGAGCA TCTCCTGAAGGAGAAGGAGCTCCTCATTGACAAGCAAAGGAAGCATATCTCTCAGCTGGAGCAGAAAGTG CGAGAGAGTGAACTGCAAGTCCACAGTGCCCTTTTGGGCCGCCCTGCCCCCTTTGGGGATGTCTGCTTAT TGAGGCTACAGGAGTTGCAGCGAGAGAACACTTTCTTACGGGCACAGTTTGCACAGAAGACAGAAGCCCT GAGCAAGGAGAAGATGGAGCTTGAAAAGAAACTCTCTGCATCTGAAGTTGAAATTCAGCTCATTAGGGAG TCTCTAAAAGTGACACTACAGAAGCATTCGGAGGAGGGGAAGAAACAGGAGGAAAGGGTCAAAGGTCGTG ATAAACATATCAATAATTTGAAAAAGAAATGTCAGAAGGAATCAGAGCAGAACCGGGAGAAGCAGCAGCG TATTGAAACCTTGGAGCGCTATCTAGCTGACCTGCCCACCCTAGAAGACCATCAGAAACAGACGGAGCAG CTTAAGGACGCTGAATTAAAGAACACAGAACTGCAAGAGAGAGTGGCTGAGCTGGAGACTTTGCTGGAGG ACACCCAGGCAACCTGCAGAGAGAAGGAGGTTCAGCTGGAAAGTCTGAGACAAAGAGAAGCAGACCTCTC CTCTGCTAGACATAGCTTTCAAGATAAACAGTCTGTGGAGGAAGCCAATGGAGAAAACCTCAGAGTGGAC ATGGAATCCCAGCAGAAGGAATGTGACTCCCTCCGAAAGATGGTGGAGAGGCAACAGCTGAAGATGGAAC AGCTGCACTCCCAAGTCCAGAGCCAAAAGCAAGAACTGGCACAAGAAGAGGGAATCAACCAGGCACTGAG AGAGGAGGCCCAGCGGAGGGAGACGGCCCTGCAGCAGATGCGCACAGCCGTGAAGGAGCTTTCAGTGCAA AACCAGGACCTGATTGAGAAGAATCTGACGCTGCAAGAGCACTTGCGCCAGGCCCAGCCAGGGTCCTCAT CTTCACCAGACTCAGCCCAGCTGGCCTGTGAGCTGCACCAGGAACTGGCCAGTTGCCTTCAAGATCTGCA AGCTGTCTGCAGCATTGTGACCCAGAGGGCCCAAGGCCACAATCCTAACCTCTCACTACTCCTAGGCATT CACTCCACCCAGCACCCAGGAACGCAGCTAGATCTACAAAAGCCAGATGTGATCAGGAGGAAACTAGAAG AGGTTCAACAGCTGCGCCATGACATTGAGGACTTAAGGACCAGCCTGTCAGACAGATATGCCCAGGACAT GGGAGAAAACTGTGCCACACAGTGAGGAGTGCCAGGGAGGACTTAGGCTGCTGCTGCCTCGCCAGAAGGT TCCTTCCCTGAGAGTTCTGCCTTCTGCTGCTGCACCCAGAGGGGAGAACCTGTATCTGGGCCCAGGGTTG TGAGAAGCCTGCCTGTTTTGCTGTTCTCTGGCTTTGCCTTATTGGGTTATGTTTTGCTTGTTATCTTAAT TCACTCTAAAAGACTGAGTTACTAATTAACCTTTTTGCAGATTTCAACTGATAAAGTCCTCACAAGCTGT TCCCAGCTCTAACCTGGCATGAGAAGCAAACAGGCTGCTGGCCTCCCCTCCATCCTCCAGGACGGATCCA TGTTCATTCCTATCCATACCTTGGTCCTACTGATGCTCTTTTAAGAGAGGCCTAAAGGGTAAAGTAGGAG TAGCAACCTTTTAAGAATTCTCAGGCCCCTTGTATCAGTCTTCTGAGAACTCAGCTGGCTTTCTGGGTTT TCTCAGGTACCTGTTTCCTCAGGGCTGTTGTTCCCACAGAGGCCTCAAGGTTATCCACCCCTCAGCAGAG CCCCTACTATGATCACTGACTTGGGGACCATGGCAGGGCTCCTCGGCCTGCCTGCTGGGCCATCTTTTAC AGGAAACTCTTCTTAGCTACCCATTGTCTGTCCCTAGAACCTTGGGCATGTGGGCATGTGCTTGGGTCCG CTATGTCGTCTAGGAGTACAGACGCCTCTGTGGTTGCCTCGTGGTTCCCGGGTTGGACATGAGTTTCCCC CGTAAGAGCACAGGGTGGCTGTGGCTGCTCAGTCCTTCCTAAGGCTGGGCGCTGTCGCTGTCCTCTGAAC TGTGCCTCTCATTTGGGGTTAGGAGGGTTCCCTTTTCCTGCTCTAACCTTGACAGTTCGGAGCACCTAAG GTAAAAAAGTGTTGAGTGTTTTCTGGTGTTTTGTGTTTCTCTAAGGCCTTTGAGCCTTAGCTTTCTGGTT TTTCTTGCCTCCCAATTTAGGGATTGTCTCAGTTGTATCTGGTTATTTGGCCAGCCCTCAACCTCCTCTA GTTTGGATCCAAGGGCCCTGTATGTATGAGGAGTAAAGTAGTTGTGGGGGTGCAGACAGGACTTCTGCTA AGCCTTGGGGCTGTTAACAGCACCGTGGACCTCCAGGGCCTGAGGGGCCGCTTTGCCAGTGTTTTAACAA GTTTATGTTTTCTTATGGAGTGGCTTACCTTTCAGAGTAATCACACCCAGCTGACCATCCCCTCAGCCTC CGGGCTGCTGCCTCCTTAAAACAGAGCTTCCTGGTTGGTTTCCACCCTGTTATCGGGAGCCCAGCCTCCA TGCTCACGGGTATTTTTCTTCGTAAAGTTTATAAAGTTATTTATATGAATCTTTGTTAAGTCGACTGGTT TGTATTGCCTATTTTGATTCCAGAATAAACCCTTTTCCCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Restriction Sites RsrII-NotI |
ACCN NM_144527
Insert Size 2280 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC031729, AAH31729
RefSeq Size 3783 bp
RefSeq ORF 2280 bp
Locus ID 70012
UniProt ID Q8BMK0
Cytogenetics 4 D3
Summary Acts as a negative regulator of NEK2 to maintain the centrosome integrity in interphase. Suppresses centrosome disjunction by inhibiting NEK2 kinase activity.UniProtKB/Swiss-Prot Function
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"NM_144527" in other vectors (4)
SKU Description Size Price
MG210523 Cep85 (tGFP-tagged) - Mouse coiled-coil domain containing 21 (Ccdc21) 10 ug
$897.00
MR210523 Cep85 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 21 (Ccdc21) 10 ug
$589.00 $697.00
MR210523L3 Lenti ORF clone of Cep85 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 21 (Ccdc21) 10 ug
$997.00
MR210523L4 Lenti ORF clone of Cep85 (mGFP-tagged) - Mouse coiled-coil domain containing 21 (Ccdc21) 10 ug
$997.00

file-alt Citations

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