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Adar (NM_019655) Mouse Untagged Clone

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SKU
MC204455
Adar (untagged) - Mouse adenosine deaminase, RNA-specific (Adar), transcript variant 1, (10ug)
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ORF Plasmid:
Tag Free DDK
  $1,004.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Adar
Synonyms Adar1; Adar1p110; Adar1p150; AV242451; mZaADAR
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC042505
GCCACAGGTGCGGGCCTTGCCGGCACTATGTCTCAAGGGTTCAGGGGACCCACAGGGGTGTTCCCTCACC AGACACAGTCGTACTCGGACCCTAGTCATGAGCATAGCAAGTGGAGATACCTGCAGCCACAGGGGCCGGA GTCTTACCCTAGGAGTTTCCAGCTTCAGCAGATAGAGTTTCTCAAAGGGCGGCTCCCAGAAGCTCCCTTG ATTGGAATACAAACCCAGTCACTGCCGCCATTCCTCCCAGGACACTGGCCAAGATTCCCAGGGCCACCTG CCCAAGACAGGCAACTGGAAATCTGGGAGTTCCCCAGGAGTGTGACTCTCAGAAATCAGGGGTTCCACAT AGGACCCCCACTTCCTCCCCCACACAGCAGGGGTCCACCATGGAGAGGTGCTGACGGGCTTTGCTCACAC TTCCGGGAGCTGAGCATCAGTCAGAGTCCGGAGCAGAAGGTTCTAAACCGCCTGGAAGAGCTTGGGGAGG GGAAGGCCACCACTGCCCATGTGCTAGCCAGAGAGCTCAGAATCCCCAAAAGGGACATCAATCGTATTTT GTACTCCCTGGAAAAGAAGGGAAAGCTGCACAGAGGAAGGGGGAAACCTCCTTTGTGGAGCCTTGTGCCC TTGAGTCAGGCTTGGACTCAGCCCCCTGGAGTTGTGAATCCAGATAGTTGTATCCAGGAATTCCCTAGAG GAGAGCCTGGTTTGGACAGTGAGGACGGAGACCCTGCCTCTGACTTAGAAGGACCTTCTGAGCCTCTTGA CATGGCTGAAATCAAGGAGAAGATCTGTGACTATCTGTTCAATGTGTCAAACTCCTCTGCCCTGAACCTG GCTAAGAACATTGGCCTCACCAAGGCCCGAGATGTGACCTCAGTGCTGATTGACTTGGAAAGGCAAGGCG ATGTCTACAGGCAAGGGGCAACTCCTCCCATCTGGTACTTGACGGACAAGAAGCGTGAGAGGCTGCAGAT GAAGAGAAGTACACACAGTGCTCCTGCCCCTACCCTGACAGCTGTCCCAGAGGCCACTAGAAGCCCCTCA TTCCCTGCCTGCCACCCGCCCCCAGCAGGTGCCTCAAGCAGTGTGGCAGCCTCCAAGAGAGTGGAGAATG GGCAGGAGCCTGCGATAAAGCATGAAAGTAGGCATGAGGCCAGACCAGGACCAATGAGACTGCGGCCTCA CGCTTATCACAATGGCCCCTCTAGAGCAGGGTATGTGGCCTCTGAAAATGGCCAGTGGGCCACAGATGAC ATCCCAGATAACTTGAATAGTATCCACACAGCACCAGGTGAGTTTCGAGCCATCATGGAGATGCCCTCCT TCTACAGCCCTACCTTGCCACGGTGTTCACCCTACAAGAAGCTAACTGAGTGCCAGCTGAAGAACCCTGT CAGCGGGTTGTTAGAGTATGCTCAGTTCACTAGTCAGACCTGTGATTTCAACCTGATAGAGCAGAGTGGA CCGTCCCATGAACCTCGATTTAAATTCCAGGTTGTCATCAATGGGCGGGAATTTCCCCCAGCTGAGGCTG GCAGCAAGAAAGTAGCCAAGCAGGACGCAGCAGTGAAAGCCATGGCGATTCTGCTTCGGGAAGCCAAAGC CAAAGACAGTGGTCAACCAGAAGACTTGTCCCACTGTCCCATGGAAGAAGACTCGGAGAAACCAGCAGAG GCTCAGGCCCCCAGCTCCTCAGCAACATCCTTGTTCTCTGGGAAGAGCCCAGTTACTACACTACTTGAGT GCATGCACAAACTAGGGAACTCCTGTGAATTCCGTCTCCTGTCCAAAGAAGGCCCTGCTCATGACCCCAA GTTCCAGTACTGTGTAGCAGTAGGAGCCCAGACCTTCCCCCCTGTGAGCGCCCCCAGCAAGAAGGTAGCA AAGCAGATGGCCGCAGAGGAAGCCATGAAGGCGCTGCAGGAGGAGGCAGCCAGTTCAGCGGATGACCAGT CTGGAGGTGCGAACACAGACTCACTTGATGAATCTATGGCTCCCAACAAGATCAGGAGGATTGGTGAGCT CGTCAGGTACCTGAACACCAACCCCGTAGGCGGCTTGTTGGAGTACGCCCGATCTCATGGCTTTGCTGCT GAGTTCAAGCTCATTGACCAGTCTGGACCTCCTCACGAACCCAAGTTTGTTTACCAAGCAAAAGTTGGGG GCCGCTGGTTTCCAGCCGTGTGTGCACACAGCAAGAAACAGGGCAAGCAGGATGCAGCGGATGCAGCCCT CCGGGTCTTGATCGGGGAGAGCGAGAAGGCAGAGCAGTTGGGTTTCGCAGAGCTTCCTCTCTCTGGCAGC ACCTTCCACGACCAGATAGCTATGCTGAGCCACAGGTGCTTCAATGCTCTGACCAACAGTTTCCAGCCCT CCCTGCTCGGCCGCAAGATCCTGGCTGCCATTATTATGAAAAGAGATCCAGAGGACATGGGTGTTGTCGT GAGTTTGGGGACAGGCAATCGCTGTGTGAAAGGGGACTCTCTCAGCCTGAAGGGAGAGACGGTCAATGAC TGCCATGCCGAAATCATCTCCCGGAGGGGCTTCATCAGGTTTCTCTACAGTGAACTGATGAAGTACAACC ACCACACTGCCAAGAACAGCATATTTGAGCTTGCCAGGGGAGGAGAGAAGCTGCAGATAAAGAAGACGGT TTCTTTTCATCTCTACATCAGCACGGCGCCATGTGGAGATGGAGCCCTCTTTGACAAATCCTGCAGTGAC CGTGCCGTGGAAAGCACAGAGTCCCGCCATTACCCTGTCTTTGAAAATCCCAAGCAAGGCAAGCTTCGCA CCAAGGTGGAGAATGGGGAAGGCACAATTCCTGTGGAGTCCAGTGATATTGTACCCACGTGGGATGGCAT CCGCCTTGGGGAAAGACTCCGTACCATGTCCTGTAGTGACAAAATCCTACGCTGGAATGTGCTGGGCCTG CAAGGGGCGTTGTTGACGCACTTCCTACAGCCTGTGTACCTGAAATCTGTAACCTTAGGTTACCTTTTCA GCCAAGGGCATCTGACCCGTGCTATTTGCTGCCGCGTGACCAGAGATGGGAAAGCATTTGAGGATGGACT ACGCTATCCCTTTATTGTCAACCACCCCAAGGTCGGCCGAGTCAGTGTTTATGATTCCAAAAGACAGTCC GGAAAGACCAAGGAAACAAGCGTCAACTGGTGCATGGCTGATGGCTATGACCTAGAGATCCTGGATGGCA CCAGAGGCACTGTGGATGGACCAGGGAAAGAGTTGTCTCGGGTGTCCAAGAAGAATATTTTCCTTCAGTT TAAGAAGCTCTGCTCCTTCCGAGCCCGCAGAGATTTACTGCAGCTCTCTTATGGTGAAGCCAAGAAAGCT GCCCGTGACTATGACTTAGCCAAGAACTACTTCAAGAAAAGCCTGCGAGACATGGGCTATGGGAATTGGA TCAGCAAACCCCAGGAGGAAAAGAACTTTTACCTCTGTCCAGTACCCAATGACTGATAGTGGGGCGCGTT TCTCCTGGGGTCAGAGGGCGGTCATGGCATTCCTCATCACACCGGGCCAGAGGATAGGAGCTTTTTTTAC CCACTCCCCCCTTTTTTAATGGTAGAACCATAATAGATGGTACCAAGAACTGCTTTCTTTGGGATTTCGA GGTGGGGTCCAGCCAAGTCCCCACCTCCCTTTTCTCAAGGGAAGAGGCCAAGATTAAAGGAAATGGAAAT GCTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_019655
Insert Size 3459 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC042505, AAH42505
RefSeq Size 3764 bp
RefSeq ORF 3459 bp
Locus ID 56417
UniProt ID Q99MU3
Cytogenetics 3 F1
Summary Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Does not affect polyomavirus replication but provides protection against virus-induced cytopathic effects. Essential for embryonic development and cell survival and plays a critical role in the maintenance of hematopoietic stem cells.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) uses a different splice site, compared to variant 3. The resulting protein (isoform 1) is shorter when it is compared to isoform 3.
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"NM_019655" in other vectors (3)
SKU Description Size Price
MR211714 Adar (Myc-DDK-tagged) - Mouse adenosine deaminase, RNA-specific (Adar), transcript variant 1 10 ug
$589.00 $1,004.00
MR211714L3 Lenti ORF clone of Adar (Myc-DDK-tagged) - Mouse adenosine deaminase, RNA-specific (Adar), transcript variant 1 10 ug
$1,304.00
MR211714L4 Lenti ORF clone of Adar (mGFP-tagged) - Mouse adenosine deaminase, RNA-specific (Adar), transcript variant 1 10 ug
$1,304.00

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